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The expression of MPO,TGF-β1 and CD15 in the colon cancer's tissue

Lifang SUN ; Songlin LI ; Xiurong LI ; Yanying WANG ; Zhongqing XU ; Xiaoguang MAO

Chinese Journal of Microbiology and Immunology.2009;29(8):758-761. doi:10.3760/cma.j.issn.0254-5101.2009.08.024

colon cancer and to find out treatment and prevention by using different gene changes.

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Analysis of Alternaria alternuta allergen by 2-diamensional electrophoresis

Naiqing CAO ; Hongyu ZHANG

Chinese Journal of Microbiology and Immunology.2009;29(8):688-690. doi:10.3760/cma.j.issn.0254-3101.2009.08.005

alyzing allergen of Alternaria alternata.

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Effect of recombinant human WN-α and cisplatin on cell apoptosis and invasion in MG-63 cell

Tingting DING ; Jun ZHAO ; Jingxian CHEN ; Zongsheng YIN ; Guofu CHEN ; Mingli WANG

Chinese Journal of Microbiology and Immunology.2009;29(8):753-757. doi:10.3760/cma.j.issn.0254-5101.2009.08.023

of MG-63 cells to cisplatin,and can inhibit the invasion and metastasis of MG-63 cell.

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Screening of renal Epstein-Barr virus infection in patients with lupus nephritis

Xiaoxia YU ; Huafeng LIU ; Jingli TAO ; Chen YANG ; Shangmei LI ; Xiaowen CHEN ; Dong LIANG ; Deshen TANG

Chinese Journal of Microbiology and Immunology.2009;29(8):737-741. doi:10.3760/cma.j.issn.0254-5101.2009.08.019

V infection group than in no infection group,while positive rate of other autoantibodies was not statistically different between the two groups.Conclusion Renal EBV infection may involve in the pathogenesis of LN by inducing the production of anti-Sm-Ab.

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Characterization of promoter of human interferon regulatory factor 3

Huaguo XU ; Wei PEN ; Chao LU ; Guoping ZHOU

Chinese Journal of Microbiology and Immunology.2009;29(8):732-736. doi:10.3760/cma.j.iasn.0254-5101.2009.08.017

ays revealed that the-(167-111)bp region Was the minimal promoter of the human IRF3 gene.These results suggested that transcriptional factors such ag E2F might be involved in the transcriptional regulation of IRF3 gene.

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Interleukin 17 level changes in different stages of Coxsackie virus-induced myocarditis in mice

Song LIN ; Weifeng WU ; Yanlan HUANG ; Gong LI ; Shaodong TANG

Chinese Journal of Microbiology and Immunology.2009;29(8):727-731. doi:10.3760/cma.j.issn.0254-5101.2009.08.016

lso.This result suggested that Th17 subset is differentiated in chronic stage of viral myocarditis.

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Targeting knock out of Leptospira interrogans flagellum-associated fliN gene and pathogenic function alteration of the mutant

Hongqiang LOU ; Sumei LIAO ; Ye HU ; Jie YAN

Chinese Journal of Microbiology and Immunology.2009;29(8):677-682. doi:10.3760/cma.j.issn.0254-5101.2009.08.001

plasmid can be used to study the pathogenic mechanism of target gene products of L.interrogans.

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Construction and identification of plasmid with luciferase reporter gene for detection of T-bet expression activity

Peng GAO ; Shuai GUO ; Taiping SHI ; Dalong MA

Chinese Journal of Microbiology and Immunology.2009;29(7):650-655. doi:10.3760/cma.j.issn.0254-5101.2009.07.018

Objective To construct a T-bet response reporter gene, for the detection of T-bet tran-scriptional activity and application in high-throughput screening for the functional genomies. Methods The cis-acting DNA element, ThRE, based on CNS-22 T box site of IFN-γ gene, was recombined into a reporter vector pLUC-MCS. The reporter gene was transfected into HEK 293T cells to detect its response to T-bet. And the binding of T-bot to TbRE was identified with electrophoretic mobility shift assay(EMSA). Results ThBE was successfully cloned into pLUC-MCS, named as TbRE-LUC. Using a luciferase assay, expression of the reporter gene is found to be induced by T-bet in a dose dependent manner and correlate with T-bet ex-pression positively with activation up to 20 folds. Moreover, the binding specificity of T-bet to TbRE is vali-dated by EMSA. Conclusion We successfully constructed a T-bet response reporter gene, ThRE-LUC, which responds to T-bet keenly and specifically. TbRE-LUC will be a useful tool in high-throughput screen-ing for human genes associated with transcription activity of T-bet.

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Clinical significance of anti-β2glycoprotein I antibodies in systemic lupus erythematosus

Weidong JIN ; Limin GONG ; Yongjian CHEN ; Fang SU ; Yasong LI

Chinese Journal of Microbiology and Immunology.2009;29(7):646-649. doi:10.3760/cma.j.issn.0254-5101.2009.07.017

Objective To investigate the clinical application of anti-β2 glyeoprotein Ⅰ antibodies (IgG, IgM, IgA)in systemic lupus erythematosus(SLE). Methods The anti-β2-GP Ⅰ antibodies and anti-cardiolipin antibodies(ACL) level were measured by ELISA in 100 SLE patients, 39 other rheumatoid arthri-tis patients and 30 healthy control people. Their clinical application was analyzed in SLE diagnose and thera-py. Results The level of anti-β2-GP Ⅰ (IgG, IgM, IgA)were significantly higher in SLE than that in healthy (P < 0. 01 ). Sensitivity, specificity, positive predictive value and negative predictive value were 17.2%, 95.7%, 85.0% and 44. 6%, respectively. There was a significant and positive correlation be-tween anti-β2-GP Ⅰ antibodies and ACL antibodies ( IgG, IgM, IgA) (r = 0.418, 0. 624, 0.518, 0. 583, P <0.01). In multivariate analysis the factors(anti-β2-GP Ⅰ antibodies, ACL antibodies, dsDAN, u1-RNP, Sm, SSA, SSB, Jo-1, Scl-70, P-protein, PT, APTT) associated with SLE disease activity index(SLEDAI) were anti-β2-GP Ⅰ (IgG) and dsDNA. Conclusion anti-β2-GP Ⅰ antibody has high specificity and positive predict value, also is associated with SLE's thrombosis. It has some values in the clinical application.

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STR profiling on authentication of human cell lines for production

Shufang MENG ; Yu WU ; Jianping FENG ; Xueling WU ; Youchun WANG ; Defu LI

Chinese Journal of Microbiology and Immunology.2009;29(7):636-641. doi:10.3760/cma.j.issn.0254-5101.2009.07.015

Objective To study the application of short tandem repeat (STR) profiling in quality control of human cell lines used for biological production. Methods The methods detecting 9 and 16 STR loci to identify human cell lines by PCR-capiilary electrophoresis were established respectively. Human cell lines, which were derived from many corporations and including diploid cell strains used for virus-vaccine production and 293 cell lines used for gene therapy products, were analyzed and compared by these two methods. Results The STR profiling methods used for authentication of human cell lines were established. Most of human diploid cell strains(20/21 ) used for virus-vaccine production from 13 corporations were iden-tiffed as the intended cells and no cross-contamination was found. However, one MRC-5 cells was identified as a false cell line and one MRC-5 had 3 alleles in D13S317 locus. For 12 strains of 293 cell lines, there were significant differences in STR profiling from different manufactures, which was likely be explained that the sources and gene modifications of these 293 cell lines are not well known and their genes are unstable during passage. Conclusion The STR profiling method has the advantages of high sensitivity and specifici-ty, and can be used for authentication of each of human cell lines for biological production.

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