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Influence of endothelial cells on the proliferation of scar-derived fibroblast in hypertropic scar tissue.

Linxi ZHANG ; Shuzhong GUO ; Wensen XIA ; Li YANG ; Zhen WANG

Chinese Journal of Plastic Surgery.2002;18(6):338-340.

OBJECTIVETo investigate the influence of the endothelial cells on the scar-derived fibroblasts in the hypertropic scar tissue.

METHODSThe endothelial cell from the human umbilical vein was cultured and its supernatant was then collected. Thereafter, it was mixed with the pre-cultured scar-derived fibroblasts from the hypertropic scar tissue. The fibroblasts were analyzed with a flow cytometer in a MTT-assay method to evaluate the cell proliferation and its division cycle.

RESULTSThe endothelial cells were significantly increasing the scar-derived fibroblast proliferation (P < 0.05), compared with the control. The proportion of the fibroblasts in the S-stage of cell cycle was also quite high.

CONCLUSIONThe endothelial cells may secrete some active products, which may enhance the proliferation of the scar-derived fibroblasts and stimulate the fibroblasts into the S-stage of cell generation cycle.

Cell Cycle ; drug effects ; Cell Division ; drug effects ; Cell Line ; Cells, Cultured ; Cicatrix, Hypertrophic ; pathology ; Culture Media, Conditioned ; chemistry ; pharmacology ; Dose-Response Relationship, Drug ; Endothelium, Vascular ; chemistry ; cytology ; Fibroblasts ; drug effects ; pathology ; Humans

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The distributing characteristics and proliferating activity of fibroblasts from the surrounding skin of keloids.

Hongjie DUAN ; Jianhua GAO ; Tao LEI ; Yongbo LIU ; Feng LU

Chinese Journal of Plastic Surgery.2002;18(6):335-337.

OBJECTIVETo investigate whether there are abnormal fibroblasts derived from the surrounding skin of keloids so that a more accurate therapy for keloids could be obtained.

METHODSSamples were taken for cell culture. When primary cells fully covered the culture bottle, the shape and distributing characteristics of fibroblasts were observed under the light microscope. 6-8 passage fibroblasts were selected for comparing the proliferating activity by MTT contrasting color method.

RESULTSThe fibroblasts have the same shape in all groups. But the fibroblasts derived from the surrounding skin grow crossly and overlapped just as the fibroblasts from keloids. The proliferating activity of the fibroblasts from surrounding skin is not as high as that from the border of keloids, but is higher than the normal skin fibroblasts derived either from a normal person or a patient with keloid.

CONCLUSIONIt is likely that there are abnormal fibroblasts in the surrounding skin of keloids.

Adolescent ; Adult ; Cell Division ; Cells, Cultured ; Female ; Fibroblasts ; pathology ; Humans ; Keloid ; pathology ; Male ; Skin ; pathology

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Repair of large facial defect with an expanded clavicular-pectoral skin flap.

Zhifei LIU ; Qun QIAO ; Qixu ZHANG ; Jiaming SUN ; Jing GAO ; Yu ZHAO ; Ying YUE ; Guangci SUN

Chinese Journal of Plastic Surgery.2002;18(6):330-332.

OBJECTIVETo search for an ideal method to repair large facial defects.

METHODSOne or two soft tissue expanders were implanted at the clavicular-pectoral region. After fully expansion, a clavicular-pectoral skin flap was designed and created with similar color, texture, and size to the facial defect. The flap would pass over the neck and transfer to the face to repair a large defect.

RESULTS10 cases were treated with this method. All have been followed up for 12-18 months with satisfactory results.

CONCLUSIONThe large facial defects could be repaired by the expanded clavicular-pectoral skin flap without damaging the natural neck contour and donor site abnormality was avoided. The patients get an aesthetic look and good function.

Adolescent ; Adult ; Child ; Clavicle ; Face ; abnormalities ; surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Pectoralis Muscles ; transplantation ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; methods ; Surgical Flaps ; Treatment Outcome

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Correction of lower eyelid retraction following lower eyelid blepharoplasty.

Xin XING ; Tianxiang OUYANG ; Jianxing SONG

Chinese Journal of Plastic Surgery.2002;18(6):327-329.

OBJECTIVETo investigate the ideal methods for correction of lower eyelid retraction following lower eyelid blepharoplasty.

METHODSTranscanthal canthopexy was used in 5 patients (8 eyes) with mild lower eyelid retraction. Transcanthal canthopexy combined with Hamra's lower blepharoplasty was used in 15 patients (27 eyes) with severe lower lid retraction.

RESULTS14 patients (25 eyes) were followed up for 6-12 months. Of them, 13 patients achieved satisfactory results; one patient had undercorrection of retraction.

CONCLUSIONTranscanthal canthopexy is a simple and effective method for correction of mild lower eyelid retraction following lower eyelid blepharoplasty. Transcanthal canthopexy combining Hamra's lower blepharoplasty may be an ideal choice for correction of severe lower eyelid retraction.

Adult ; Blepharoplasty ; adverse effects ; Eyelid Diseases ; etiology ; surgery ; Eyelids ; surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Patient Satisfaction ; Surgery, Plastic ; methods ; Treatment Outcome

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The expanded distant skin flap and its application.

Kaihua LU ; Yufeng AI ; Shuzhong GUO ; Xianjie MA ; Hui ZHANG ; Linxi ZHANG

Chinese Journal of Plastic Surgery.2002;18(6):325-326.

OBJECTIVETo summarize and present the experiences and results of the expanded distant skin flaps used in plastic and reconstructive operations.

METHODS102 patients who had undergone expanded distant skin flap transfer were reviewed and followed up.

RESULTSGood results were obtained. Especially when there was no sufficient normal skin close to the lesion or defect, the distant skin flaps were employed. The expanded distant skin flap can be directly transferred as a pedicle skin flap or an island skin flap. It can be indirectly transferred with an intermediary carrier or as a free skin flap.

CONCLUSIONThe expanded distant skin flaps have many advantages, indications, worthy of recommendation.

Humans ; Skin Transplantation ; methods ; Surgery, Plastic ; methods ; Surgical Flaps

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Computer-aided tissue expansion 3D profilometry and surgery planning system.

Hui ZHANG ; Kaihua LU ; Yan HAN ; Shuzhong GUO ; Yufeng AI ; Ying NI ; Feng GUAN

Chinese Journal of Plastic Surgery.2002;18(5):305-307.

OBJECTIVETo develop and evaluate the computer-aided system in measurement of expanded skin and preoperative planning.

METHODSStereophotogrammetric technique was used to gain the 3D image-pairs, from which the contours of the expanded sites were restored. The 3D surface data were provided to the specially developed "computer-aided tissue expansion 3D profilometry and surgery planning system", to calculate the expansion area and help the preoperative design.

RESULTSThe system has been applied clinically in 16 tissue expansion sites of 11 patients with fairly good results since March 1999. Compared with the traditional method, this system is accurate, repeatable and feasible.

CONCLUSIONThis technique is useful and promising for improving the operation of tissue expansion.

Adult ; Dermatologic Surgical Procedures ; Humans ; Imaging, Three-Dimensional ; methods ; Male ; Skin ; injuries ; Surgical Flaps ; Tissue Expansion ; Tissue Expansion Devices

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The influence of expansion maintenance period upon in vivo skin tension and immediate stretch-back rate of the expanded skin.

Xuejun LIU ; Guangci SUN ; Yuehua WANG

Chinese Journal of Plastic Surgery.2002;18(5):302-304.

OBJECTIVEThe study was to investigate the influence of maintenance period of expansion upon skin tension and immediate stretch-back rate of the expanded skin.

METHODS30 expanders (240 ml) were implanted in six adult dogs, which were divided into four groups: group A with 2-week saline injection of the expander; group B with 6-week injection; group C with sham-operation and group D as the blank control group. The first two groups were subdivided into three groups respectively according to different maintaining times of one week, two weeks and four weeks. When full inflation was finished, a 4 cm x 7 cm expanded flap was created and in vivo skin tension and stretch-back ratio of the flap were measured. The Comparison was made in terms of in vivo skin tension and immediate stretch-back ratio between different injection and different maintenance periods.

RESULTSWith expansion maintenance time increasing, the in vivo skin tension and immediate stretch-back ratio decreased. After the capsule of the expand skin flap was removed, the in vivo skin tension and immediate stretch-back rate significantly reduced. Based on the experimental results, 16 patients were treated with implantation of 23 expanders. Their average expander injection time was 14 days and the average maintenance time was 25 days. All expanders obtained good results except one extrusion.

CONCLUSIONIn skin and soft tissue expansion, in vivo skin tension and immediate stretch-back ratio can be reduced through prolonged maintenance of expansion and capsule removal from the expanded skin flap.

Adolescent ; Adult ; Animals ; Biomechanical Phenomena ; Child ; Dogs ; Female ; Humans ; Male ; Reconstructive Surgical Procedures ; methods ; Skin ; physiopathology ; Surgical Flaps ; Time Factors ; Tissue Expansion ; methods ; Tissue Expansion Devices

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An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF.

Zhigang ZHOU ; Qinglian HE ; Zihao LIN ; Songlin YANG ; Xiaohai ZHU

Chinese Journal of Plastic Surgery.2002;18(5):299-301.

OBJECTIVETo investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF.

METHODSCultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c-myc mRNA expression were detected.

RESULTSThe expression of c-myc mRNA in the bFGF-treated groups increased (P < 0.05) with a dose- and stimulating time-dependent manner. The c-myc mRNA expression reached to a peak level at 2 hours.

CONCLUSIONSC-myc expression may be an important component in controlling the transit of cells through the cell cycle.

Animals ; Cell Division ; drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelium, Vascular ; cytology ; drug effects ; metabolism ; Fibroblast Growth Factor 2 ; pharmacology ; Gene Expression Regulation ; drug effects ; Genes, myc ; genetics ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Time Factors

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Surgical depilation in low hairline aurical reconstruction.

Yong TANG ; Hongxing ZHUANG ; Qinghua YANG ; Juan HAN ; Yanyong ZHAO

Chinese Journal of Plastic Surgery.2002;18(5):297-298.

OBJECTIVELow hairline is one of the most troublesome problem in auricle reconstruction. There was no satisfactory way to manage the problem. This article discuss surgical depilation to deal with this problem and reconstructed auricle.

METHODSAccording to the degree of the low hairline, Postaurical scalp was expanded and part of the lower follicles within the dermal were removed, or postaurical scalp that part of lower follicles and dermal had been removed was expanded and covered skeleton of ear with this expanded skin to reconstructed auricle.

RESULTSAuricles were reconstructed with this method in 152 lowhairline microtial cases, the result was very satisfactory.

CONCLUSIONThis procedure is an effective way to deal with low hairline in reconstructing auricle.

Adolescent ; Adult ; Child ; Ear, External ; abnormalities ; surgery ; Female ; Hair Removal ; methods ; Humans ; Male ; Reconstructive Surgical Procedures ; methods ; Scalp ; surgery ; Surgical Flaps ; Tissue Expansion

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Analysis of 5459 cleft lip and palate cases.

Bin XIONG ; Min ZHAO ; Ting CHENG ; Pu GAO

Chinese Journal of Plastic Surgery.2002;18(5):294-296.

OBJECTIVETo analyse the constitution of 5459 cleft lip and palate cases and to review the development of surgical treatment to them.

METHODSThe data of the 5459 cleft lip and palate cases were collected and classified. The ratio of sex and disease pattern were calculated and analyzed statistically. Also, by means of classification of the disease pattern, the operation methods and the operation time, the change of the surgical methods and the first operation age were analyzed.

RESULTSIn the cleft lip and palate cases, the sex ratio is different. There were obvious differences in the first operative ages in different periods. The main repair methods for cleft lip and palate changed with time.

CONCLUSIONThe male is more than the female in the cleft lip and palate patients. The average first operation age has been decreased and the main repair methods have changed since 1980.

China ; epidemiology ; Cleft Lip ; epidemiology ; pathology ; surgery ; Cleft Palate ; epidemiology ; pathology ; surgery ; Female ; Humans ; Male ; Sex Factors ; Statistics as Topic

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