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Gastrointestinal Hormones and Oxygen Free Radicals in IBS Rabbits of Spleen Deficiency

Journal of Zhejiang Chinese Medical University.2006;0(05):-.

[Objective] To observe the effect of Leweiyin of traditional Chinese compounds prescription on gastrointestinal hormones and oxygen free radicals in IBS rabbits of spleen deficiency,and to explore the possible mechanism for the treatment of IBS with spleen deficiency.[Methods] 36 two-month-old rabbits with white feather and black eyes(WHBE rabbit),half male and half female,were randomly divided into 6 groups:normal group,model group,Xiangsha Liujun pill group,Lizhu Changle group,low-dose Leweiyin-treated group,high-dose Leweiyin-treated group.Except the normal group,others were made into IBS model of spleen deficiency which was induced by damp heat stress and gavage with Fanxieye as well as method of either hunger or gorge.The drug groups were administered preventively from the medium-term of the model reproduction to the end of modeling.The rabbits of each group were observed.NO,NOS,MDA,SOD in serum and SP in colonic membrane were detected.[Results] NO,NOS,MDA in serum and SP in colonic membrane significantly more increased than the normal group(P

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Establishment of Immunoblotting in Detecting COX Protein and Its Manipulation

Yi LIANG

Journal of Zhejiang Chinese Medical University.2006;0(03):-.

[Objective] To introduce a modified method for immunoblotting in detecting protein and to show all the details needing to be paid attention to. We take COX-2 for example. [Method] Total proteins were extracted and quantified. Denatured proteins were separated by SDS-PAGE and were transferred semi-dryly to NC membrane, then the latter was bound with specific antibody. Finally, protein expression was detected by ECL.[Result] The method was concise and easy to manipulate. The method had been successfully used to detect COX-2 protein in our lab and the pictures were reproducible. [Conclusion] Every step of the method is described in detail. It is helpful for novice to carry out immunoblotting. This established method is worthy to be recommended.

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Moxibustion at Popliteal Transverse Line End Treating Low Back Pain

Yuhua WANG ; Baocai WANG ; Lili GONG

Journal of Zhejiang Chinese Medical University.2006;0(05):-.

Objective To seek better method to cure the low back pain. Methods To give moxibustion on the end of the popliteal transverse line on both knee at mean time, compared with routine methods in our clinical experience.Results The patients which received moxibustion had better effects than the bleeding on Weizhong and treated with differentiating signs;for statistical data, P

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Analysis on Chronic Diseases of Respiratory System Treated by“Winter Disease Being Cured in Summer”

Xiaowei ZHENG

Journal of Zhejiang Chinese Medical University.2006;0(02):-.

[ Objective]To observe the curative effects on chronic diseases of respiratory system treated by “winter disease being cured in summer”.[Methods]One hundred and twenty-six patients with chronic diseases of respiratory system were treated by herbal medicine,points application of medicament,and ear points.All the patients were observed and reported after the treatment in detail.[Results]The total effective rate was 90.5%,and with satisfactory effect in long run.[Conclusion]It is reasonable to treat chonic diseases of respiratory system in this way.

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Clinical Observation of Integrated Traditional Chinese and Western Medicine in the Treatment of Viral Myocarditis Accompanied with Ventricular Arrhythmia

Yuezhou QIAN

Journal of Zhejiang Chinese Medical University.2006;0(05):-.

0.05), and both of the integrated traditional Chinese and western medicine groups were better than that of western medicine group(P

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Study for the NiTai-JuBinQi Cure Smash Fracture of Patella

Provonce ZHEJIANG

Journal of Zhejiang Chinese Medical University.2007;0(01):-.

[Objective] To study the effective cure method for the smash fracture of patella.[Method]:Randomly divide 60 cases of the smash fracture patella into A Group(NiTai-JuBinQi)and B Group(Steel wire Round Tie),observe the clinical effect.[Result]A Group the excellent rate was 85.7%,B Group the excellent rate was 71.4%,(P

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Routine Seminal Fluid Data Analysis of 128 Cases of Unsterility Seamen

Weijian WU ; Yongshan SUO ; Lingling YE

Journal of Zhejiang Chinese Medical University.2006;0(05):-.

[Objective] To analyse seminal fluid quality of seamen with unsterility,discuss the concerned environmental influencing factors.[Method] Take 128 seamen as research group,100 drivers as control;apply the Qinghua Tongfang Auto Analytic Instrument to routinly test the seminal fluid.[Result] For the seamen with unsterility,the important indexes of spermatozoon concentration,activity rate and level-a ratio were much reduced(P

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Review on Research of Special Thrombocytopenic Purpura

Dongqi YAN

Journal of Zhejiang Chinese Medical University.2007;0(01):-.

Special thrombocytopenic purpura belongs to "blood sign and spots" in TCM.Starting from TCM,combining with concerned documents over recent 10 years,it sums up its causa morbi,mechanism,differentiation of signs and types and treatment,and discusses the state quo and main application of the combination of TCM and WM in the said disease,therefore puts forwards the advantages of combination of TCM and WM,as well as the characteristic of TCM treating the disease,finally makes analysis and prospect on the shortcomings.

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Effect of Tetramethylpyrazine on the Function of Endothelial Progenitor Cells in vitro

Jie LIN ; Lixin XU ; Deye YANG

Journal of Zhejiang Chinese Medical University.2006;0(05):-.

[Objective] This study was to investigate the effect of TMP on function of EPCs in normal or hydrogen peroxide(H2O2)inducing injury model cell culture,and to approach a therapy to coronary heart disease(CHD).[Method] Total mononuclear cells were isolated from the cord blood by ficoll density gradient centrifugation,cultured in vitro.After the cells cultured for 7 days,identified them by cellmarkers and the ability of intaking the ac-LDL,and made sure they were EPCs.To divide the cells into three groups,TMP(5,25,100,200mg),H2O2(500??)model and TMP together with H2O2,cultured for 24h and then detected the function and apoptosis of EPCs.[Result] Versus to control group,the TMP(5,25,100mg)groups had no important effect on the proliferation and adhesion of EPCs,but TMP(200mg)group had negative effect on EPCs.Versus to H2O2 model group,TMP(5,25,100mg)preincubated groups had important protective effect on EPCs in proliferation,adhesion and apoptosis.[Conclusion] TMP could protect EPCs from oxidative stress injury,but had little benefit to EPCs in normal incubation.

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Pro.Guo Xiuqin’s Experience in Treating Dementia with Yiqi Congming Decoction

Zhengliang SONG ; Xiuqin GUO

Journal of Zhejiang Chinese Medical University.2007;0(01):-.

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