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Characteristic changes of acetylcholine receptor in rat lateral pterygoid muscles after functional mandibule advancement.

Kaiyun CHEN ; Songjiao LUO

West China Journal of Stomatology.2003;21(5):400-402.

OBJECTIVEThe purpose of this study was to evaluate the maximum binding(Bmax) and affinity(Kd value) changes of acetylcholine receptor (n-AchR) in rat lateral pterygoid muscles after functional mandibule advancement.

METHODS40 five-weeks-old male Sprague-Dawley rats were equally divided into experimental and control group. The mimic functional appliances were used in experiment group and the rats were killed after 1, 3, 7, 14 days. Radio-ligand binding assay (RBA) was applied to determine the maximum binding(Bmax) and Kd value of n-AchR of lateral pterygoid muscle.

RESULTSThe Bmax of n-AchR in experimental group was higher than that in control group and the Kd value always kept in high level.

CONCLUSIONThe functional orthopedics can increase the Bmax of n-AchR in rapid growing rat lateral pterygoid muscles.

Animals ; Binding Sites ; Cell Membrane ; metabolism ; Male ; Mandibular Advancement ; Orthodontic Appliances, Functional ; Pterygoid Muscles ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Receptors, Cholinergic ; metabolism

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An experimental study on PAc and GTF gene vaccines of Streptococcus mutans against rats caries: antibody levels in saliva and serum.

Deqin YANG ; Tianjia LIU ; Fuxian CAO

West China Journal of Stomatology.2003;21(5):396-399.

OBJECTIVEThe purpose of this study is to examine the levels of salivary SIgA and serum IgG induced by pcDNA3-pac and pcDNA3-gtfB immunization, so as to testify the antigenity of the two gene vaccines.

METHODS36 28-day-old Wistar rats were divided into 6 groups, among which 3 experimental groups were vaccinated with pcDNA3-pac, pcDNA3-gtfB or pcDNA3-pac combined with pcDNA3-gtfB, respectively, one positive control was vaccinated with inactive whole cell of S. mutans JBP and other two negative controls were injected with the vector pcDNA3 or PBS buffer, respectively. All vaccines and materials were delivered with 100 micrograms by submandibular gland injection for 3 times. Then the restricted bacterial model of rat was constructed. Following that all rats were fed with cariogenic diet Keyes 2000 for 3 months, saliva and serum samples were collected to assay SIgA or IgG levels by ELASA.

RESULTSThe salivary S-IgA levels both in pcDNA3-pac combined with pcDNA3-gtfB group and inactive S. mutans cell group were higher than others (P < 0.01). In groups of pcDNA3 and PBS buffer, they were lowest (P < 0.01). The serum IgG levels in the three experimental groups and positive control were higher than that in negative control (P < 0.05). It was important that salivary SIgA in groups of gene vaccine and inactive S. mutans vaccination reached its peak at the 11th week after the first inoculation and kept until the end of the study.

CONCLUSIONBoth pcDNA3-pac and pcDNA3-gtfB can express immunogenic protein and induce immune responses of mucosal and humoral immune system in gnobobiotic rats. It is also indicated that the joint gene vaccines immunization is an optimal choice for anticaries strategy.

Animals ; Antibodies, Bacterial ; analysis ; blood ; Dental Caries ; prevention & control ; Female ; Glucosyltransferases ; immunology ; Immunoglobulin A, Secretory ; immunology ; Immunoglobulin G ; analysis ; Male ; Membrane Proteins ; immunology ; Random Allocation ; Rats ; Rats, Wistar ; Saliva ; immunology ; Streptococcal Vaccines ; immunology ; Streptococcus mutans ; immunology ; Vaccination ; Vaccines, DNA ; immunology

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Detection of the transmitted strains and non-transmitted strains of Mutans streptococci by AP-PCR.

Song LI ; Tianjia LIU ; Heng ZHUANG

West China Journal of Stomatology.2003;21(5):392-395.

OBJECTIVEDental caries is a transmissible infectious disease in which S. mutans plays the major role. The purpose of this study was to detect the S. mutans transmitted strains and non-transmitted strains by AP-PCR fingerprint for laying the foundation of study on the relation between bacterial properties of S. mutans and its transmission.

METHODSPlaque samples were obtained from buccal surfaces of 20 3-4 years old children and their mothers. Caries experience was scored by dmft (DM-FT). Diet regime, breast feeding and care of the children were recorded. 200 Isolates of S. mutans were detected by S. mutans B medium and confirmed biochemically. DNA from each isolate was purified and AP-PCR fingerprinting was conducted. Amplicons were separated by electrophoresis in 1.5% agarose gels.

RESULTS45 different patterns among the 200 isolates were found. There were 10 mothers (50%) and 15(75%) children owning one genotype while 10 mothers and 5 children owning more than one (2 mothers owning 5 types). The data showed that the mothers harbored a more heterogeneous population of S. mutans than their children. Comparisons in genotypes between children and their mothers discovered that 16(80%) children harbored the same genotypes as their mothers, indicating high transmission in the group of people. Detection of the S. mutans transmitted strains and non-transmitted strains in mothers demonstrated that 10 (50%) mothers harbored not only transmitted strains but also non-transmitted strains, suggesting that different strains had different ability of transmission.

CONCLUSIONAP-PCR was capable of detecting the S. mutans transmitted strains and non-transmitted strains. Some S. mutans genotypes had higher ability of transmission than others.

Child, Preschool ; DNA Fingerprinting ; DNA, Bacterial ; genetics ; isolation & purification ; Dental Caries ; microbiology ; Dental Plaque ; microbiology ; Family Health ; Female ; Genotype ; Humans ; Infectious Disease Transmission, Vertical ; Male ; Mothers ; Polymerase Chain Reaction ; methods ; Streptococcal Infections ; transmission ; Streptococcus mutans ; classification ; genetics ; isolation & purification

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Evaluation of in vitro cario-static effect of Galla chinensis with biofilm model.

Jiyao LI ; Zhengwei HUANG ; Xuedong ZHOU

West China Journal of Stomatology.2003;21(5):389-391.

OBJECTIVETo evaluate the in vitro cario-static effect of Galla chinesis with biofilm model.

METHODSA four-organism bacterial consortium was cultured in a biofilm model on hydroxyapatite (HA) discs in a continuous culture system and exposed to repeated solution pulsing. There were three groups with different solution pulsed in the model: negative control group was pulsed with distilled water, positive control group was pulsed with 100 mmol/L sucrose solution and experimental group was pulsed with 100 mmol/L sucrose solution containing 4.0 g/L Galla chinensis. During the experiment, the dynamic changes of pH were recorded. After 6 pulses, surface structure of the biofilm was observed with a scanning electron microscope and the population on the biofilm was enumerated.

RESULTSGalla chinesis significantly inhibited the adherence of Actinomyces naelundii to HA disc compared with the control group and facilitated the removal of acid products. It was also found that the extra-cellular polysaccharide was reduced with the pulsing of Galla chinesis.

CONCLUSIONGalla chinesis in the biofilm model can partially reduce the cario-genic response of sucrose solution.

Actinomyces ; drug effects ; physiology ; Bacterial Adhesion ; drug effects ; Biofilms ; growth & development ; Cariostatic Agents ; pharmacology ; Dental Caries ; microbiology ; Drugs, Chinese Herbal ; pharmacology ; Durapatite ; Humans ; Models, Biological ; Streptococcus mutans ; drug effects ; physiology ; Streptococcus sanguis ; drug effects ; physiology ; Sucrose ; pharmacology

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The measurement of retentive force of laser-melted and cast post-keepers of Magfit magnetic attachments.

Ling YANG ; Li DU ; Yinghe LIN

West China Journal of Stomatology.2003;21(5):386-388.

OBJECTIVEIn order to compare the influence of casting and laser welding on magnetic retentive force, the magnetic retentive forces of laser-melted and cast post-keepers of the magnetic attachments were measured in vitro.

METHODS5 cast and 6 laser-melted post-keeper specimens (Magfit MD800) were formed, respectively. Using untreated keeper as control group, the vertical magnetic retentive forces were measured by a universal testing machine.

RESULTSThe average vertical magnetic retentive force of the laser-melted post-keeper group and the cast post-keeper group in vitro were (4.223 +/- 0.217,0)N, (3.792,9 +/- 0.296,4)N, respectively. Compared with the cast post-keeper group, the average vertical magnetic retentive force of the laser-melted post-keeper groups was much higher in vitro (P < 0.05).

CONCLUSIONLaser welding had less influence on the magnetic retentive force of the magnetic attachments than casting.

Dental Alloys ; Dental Casting Investment ; Dental Casting Technique ; Dental Prosthesis Retention ; instrumentation ; Dental Soldering ; methods ; Denture Precision Attachment ; Electromagnetic Fields ; Lasers ; Magnetics ; instrumentation ; Post and Core Technique ; Stainless Steel ; Welding ; methods

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A study on microstructures and heat reaction zone of laser-melted and cast post-keepers.

Li DU ; Ling YANG ; Chun XU

West China Journal of Stomatology.2003;21(5):383-385.

OBJECTIVEIn order to introduce the laser welding technology into root-cap making, this study compared the surface characters and internal structures of laser-melted and cast post-keeper to provide experimental data for clinical application of the technique.

METHODSUsing untreated keeper as the control group, the surface characters and metallographic structures of the keepers were examined by stereomicroscope, metallographic microscope and SEM, and element analysis were conducted with EPMA.

RESULTSThe laser-melted post-keeper basically kept the original smooth and the internal structure of the keeper, the heat reaction zone was extremely small and elements diffused mutually around the fusion zone obviously. While in the cast post-keeper, the surface was rough and had an oxide-layer. The internal structure changed and a fusion band formed between the base alloy and the keeper, but element diffusion was limited to the fusion zone.

CONCLUSIONCompared with casting, laser welding technique demonstrated less influence on the surface and internal structure of the keepers.

Dental Alloys ; Dental Casting Investment ; Dental Casting Technique ; Dental Prosthesis Retention ; instrumentation ; Dental Soldering ; methods ; Denture Precision Attachment ; Electromagnetic Fields ; Hot Temperature ; Lasers ; Magnetics ; instrumentation ; Stainless Steel ; Welding ; methods

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Influence to maxillary development by repairing denudated palates with buccal mucosa and palatal mucosa.

Dawei LU ; Bing SHI ; Xu HUANG

West China Journal of Stomatology.2003;21(5):379-382.

OBJECTIVETo provide experimental data of different oral mucosa suitable to repair denudated palate.

METHODSThirty Japanese white rabbits (5 weeks old) were divided into three groups: I group (left hard palate was denudated with right side intact), II group and III group (both sides of the hard palate were denudated with right side repaired by palatal mucosa or buccal mucosa correspondingly). Then the effect of different transplanted mucosa on preventing maxillary deformity was investigated and compared.

RESULTSMaxillary development in the three groups was same and no significant differences among them were discovered.

CONCLUSIONBuccal mucosa is effective in serving as reparative tissue for preventing maxillary deformity.

Animals ; Cleft Palate ; surgery ; Female ; Maxilla ; abnormalities ; growth & development ; pathology ; Mouth Mucosa ; transplantation ; Palate, Hard ; pathology ; surgery ; Periosteum ; surgery ; Rabbits ; Random Allocation ; Wound Healing

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Correlations among mood disorder, serum interleukin-2 and interleukin-6 in patients with burning mouth syndrome.

Juan XIA ; Mei LIN ; Zaorong JIN

West China Journal of Stomatology.2003;21(5):377-378.

OBJECTIVETo examine serum IL-2 and IL-6 levels in BMS patients with or without depression and make clear the relationship between serum IL-2, IL-6 and mood disorder.

METHODSSerum IL-2 and IL-6 were examined in 48 BMS patients by means of ELISA. The Hamilton Depression Rating Scale (HDRS) was used to evaluate the depressive symptomatology.

RESULTSThere was no significant difference in serum IL-2 and IL-6 among BMS patients with or without depression. Mood disorder was not correlated to serum IL-2 and IL-6, but positively correlated to pain levels.

CONCLUSIONDepression status may not influence serum IL-2 and IL-6 levels significantly, but it has a relationship with BMS. The HDRS can be used to determine depression status in BMS patients in order to help clinical treatment.

Adult ; Aged ; Aged, 80 and over ; Burning Mouth Syndrome ; blood ; psychology ; Depressive Disorder ; blood ; psychology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Interleukin-2 ; blood ; Interleukin-6 ; blood ; Male ; Middle Aged ; Pain Measurement ; Psychiatric Status Rating Scales

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Clinical application of maxillary endossenous implant with edentulous ridge expansion technique.

Yunfei HUANG ; Yao OU ; Guangbao SONG

West China Journal of Stomatology.2003;21(5):374-388.

OBJECTIVETo evaluate the application and the effect of edentulous ridge expansion(ERE) technique in maxillary endossenous implant placement.

METHODS49 patients with maxillary alveolar ridge atrophy received edentulous ridge expansion using condenser. In order to be similar to natural root, dental implants were selected and placed to tooth missed sites according to the requirements of aesthetics, function and dimension.

RESULTS49 patients with atrophied alveolar ridge received 86 implants. The labio-lingual width augmented from 3.3 to 5.4 mm and the alveolar ridge height from 2 to 7 mm 6 months after operation. The implants osseintergrated tightly with alveolar bone and second-step prosthesis was performed 6 months after implant placement.

CONCLUSIONThe edentulous ridge expansion technique can meet the requirements of aesthetics and function and is applicable to endossenous implant placement in maxilla. The method is simple and valuable to clinical application.

Adolescent ; Adult ; Alveolar Ridge Augmentation ; methods ; Atrophy ; surgery ; Dental Implantation, Endosseous ; methods ; Female ; Gingiva ; pathology ; Humans ; Jaw, Edentulous, Partially ; surgery ; Male ; Maxilla ; Middle Aged ; Osseointegration

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Clinical analysis of the patients with burning mouth syndrome.

Yuan HE ; Mei LIN ; Gang ZHANG

West China Journal of Stomatology.2003;21(5):372-382.

OBJECTIVEClinical data of burning mouth syndrome were investigated in detail and the relation to pain was analyzed in order to find a clue to clinical treatment.

METHODS78 patients with burning mouth syndrome were examined carefully and 7 factors that related to the degree of pain were analyzed with multivariate stepwise regression.

RESULTSThe study demonstrated that the psychological disorder, autonomic nerves system abnormity, oral dryness and high-extravasated blood level were positively related to the degree of pain.

CONCLUSIONThe study suggested that psychotherapy, improvement of autonomic nerves system, treatment for oral dryness and Chinese traditional therapy of invigoration of blood circulation were of great importance in curing burning mouth syndrome.

Burning Mouth Syndrome ; diagnosis ; therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Multivariate Analysis ; Pain Measurement ; Phytotherapy ; Psychotherapy

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