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Xinxibao for the treatment of chronic bacterial prostatitis.

Dehuai LIAO ; Zhiguang CHEN ; Zhennong DENG ; Donghui WANG

National Journal of Andrology.2004;10(9):676-677.

OBJECTIVETo study the effect of Xinxibao as a supplementary drug in the treatment of chronic bacterial prostatitis (CBP).

METHODSEighty-one cases of CBP were divided into two groups: Group A (n = 38), treated with Xinxibao combined with sensitive antibiotic, and Group B (n = 43), treated with sensitive antibiotic only. Contrast studies were made on the therapeutic effects in the two groups, and the results were analyzed.

RESULTSThe effectivity rate was significantly higher in Group A than in Group B.

CONCLUSIONXinxibao can effectively relieve the symptoms of CBP.

Adolescent ; Adult ; Anti-Bacterial Agents ; therapeutic use ; Bacterial Infections ; drug therapy ; Chronic Disease ; Humans ; Male ; Metalloproteins ; therapeutic use ; Microbial Sensitivity Tests ; Middle Aged ; Prostatitis ; drug therapy ; microbiology ; Zinc ; therapeutic use

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Changes of semen quality in uremia patients.

Longgen XU ; Huiming XU ; Qizhe SONG ; Xiaoping QI ; Xinhong WANG ; Junrong ZHANG ; Li YAN ; Zongfu SHAO

National Journal of Andrology.2004;10(9):673-675.

OBJECTIVETo evaluate the changes of the semen quality in uremia patients before renal transplantation.

METHODSThe semen of 24 patients with uremia and 12 normal volunteers was analyzed.

RESULTSThe semen volume, sperm motility, survival rate, density and morphological normality percentage were (2.5 +/- 0.4) ml, (13.4 +/- 3.9)%, (25.4 +/- 5.6)%, (20.6 +/- 4.5) x 10(6)/ml and (16.8 +/- 2.1)%, respectively, significantly lower than those of the normal group (P < 0.01).

CONCLUSIONSemen qualities were lowered significantly and spermatogenesis severely affected in patients with uremia.

Adolescent ; Adult ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Semen ; physiology ; Sperm Count ; Sperm Motility ; Spermatogenesis ; physiology ; Uremia ; physiopathology

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Initial observation on L-carnitine for asthenospermia with epididymal knob.

Xuejun SHANG ; Yufeng HUANG ; Ke LI ; Wei LI ; Yichao SHI ; Guanghui LIU ; Jusheng HONG

National Journal of Andrology.2004;10(9):671-675.

OBJECTIVETo evaluate the efficacy of L-carnitine on asthenospermia with epididymal knob.

METHODSThirty-five patients aged 25-39 years with asthenospermia with epididymal knob were received at our clinic of andrology from May 2003 to July 2004, and given oral L-carnitine 2 g/d for 3 months. The sperm parameters of the patients were analyzed by computer-assisted sperm analysis system before and after L-carnitine treatment.

RESULTSOf the 32 patients who accomplished the 3-month L-carnitine therapy, only 4 remained unimproved in sperm parameters, while the other 28 were significantly improved in sperm concentration, forward sperm motility, total sperm motility, straight line velocity, curvilinear velocity and average path velocity. Four of the patients wives were pregnant and one patient fathered a child.

CONCLUSIONOral L-carnitine therapy is efficacious for asthenospermia with epididymal knob.

Adult ; Asthenozoospermia ; complications ; drug therapy ; Carnitine ; therapeutic use ; Epididymis ; pathology ; Follow-Up Studies ; Genital Diseases, Male ; complications ; drug therapy ; Humans ; Male ; Sperm Motility ; Treatment Outcome ; Vitamin B Complex ; therapeutic use

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Effect of successive ejaculation on semen analysis parameters in normal men.

Kaiyan PEI ; Yabin XU ; Mengchun JIA

National Journal of Andrology.2004;10(9):667-670.

OBJECTIVETo study the effect of successive ejaculation on semen analysis parameters in normal men.

METHODSEight ejaculates were collected at daily intervals from 8 normal men. The semen parameters were analyzed according to WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction.

RESULTSThe semen volumes and total sperm counts decreased gradually day by day, significantly lower from the 5th to the 8th day than the 1st (P < 0.01). The sperm densities also declined day by day, but not significantly. The sperm viability and motility (a + b grade) presented an increasing trend, but with no significance except on the 7th day.

CONCLUSIONWith the increase of the ejaculatory frequency, human semen volumes and the total counts significantly decrease, while sperm viability and motility gradually increase. Successive ejaculation does not affect the quality of human semen.

Adult ; Ejaculation ; physiology ; Humans ; Male ; Semen ; physiology ; Sperm Count ; Sperm Motility

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Expression of androgen receptor and heat shock protein 90alpha in the testicular biopsy specimens of infertile patients with spermatogenic arrest.

Zilong LIU ; Gang WANG ; Yan PAN ; Changhong ZHU

National Journal of Andrology.2004;10(9):662-666.

OBJECTIVETo explore the association of spermatogenic arrest with the expression of AR and HSP90alpha.

METHODSThe two-step immunohistochemical method was used to examine the expression of AR and HSP90alpha in the testicular biopsy specimens of 57 infertile men with spermatogenic arrest, compared with those taken from 15 normal healthy men.

RESULTSAR was expressed in the nuclei of the spermatogonia, spermatocytes and round spermatids of the normal testicular biopsy, but the intensity of the AR expression showed difference in each type of cells. Furthermore, the high expression of AR in spermatogonia and spermatids was on the margin of the cytoplasm, and perinuclear (forming a ring-like structure) were very distinctive in the spermatogenic arrest testicular biopsy specimens. HSP90alpha was expressed in spermatogonia, spermatocytes, Sertoli cells, Leydig cells and myoid cells in the normal testicular tissues, but highly expressed in the testicular tissues with spermatogenic arrest. The immunostaining intensity of HSP90alpha showed significant difference (P < 0.05) between the two kinds of testicular tissues.

CONCLUSIONThe results indicate that the abnormal expression sites of AR may make the androgen receptor unable to mediate androgen into the nucleus, which may down-regulate the gene transcription activity of spermatogenic cells. It is possible that the high expression of HSP90alpha may lead to a dramatic decrease in AR stability and an increase in AR abiquitirylation.

Adult ; Case-Control Studies ; HSP90 Heat-Shock Proteins ; biosynthesis ; Humans ; Immunohistochemistry ; Infertility, Male ; etiology ; metabolism ; Male ; Oligospermia ; complications ; metabolism ; Receptors, Androgen ; biosynthesis ; Spermatogenesis ; Testis ; metabolism ; pathology

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The synergistic effects of paclitaxel and gemcitabine on prostate cancer cell line PC-3.

Ming SUN ; Yuru YANG ; Hong LI ; Yirong CHEN ; Zhiping WANG ; Yiping LU ; Qiang WEI ; Zhongjin YUE

National Journal of Andrology.2004;10(9):658-666.

OBJECTIVETo observe the synergistic effects of paclitaxel and gemcitabine on prostate cancer cell line PC-3 in vitro.

METHODSCell morphology, MTU, flow cytometer and immunocytochemical method were used to observe the effects of 10(-6), 10(-7), 10(-8) mol/L paclitaxel and 10(-7), 10(-8), 10(-9) mol/L gemcitabine on prostate cancer cell line PC-3 by single or synergistic administration for 48 hours in vitro.

RESULTSGemcitabine above 10(-8) mol/L enhanced the growth suppression [suppression ratio > or = (50.8 +/- 4.2)%, P < 0.05] and apoptosis [apoptosis ratio > or = (22.9 +/- 2.3)%, P < 0.05] and down-regulation of the expression of cyclin D1 [expression ratio < or = (9.6 +/- 1.6)%, P < 0.01] induced by paclitaxel above 10(-7) mol/L in PC-3 cells. Gemcitabine changed the ratio of G2/M cell arrest induced by paclitaxel from (70.3 +/- 9.7)% to (38.2 +/- 4.2)%, and reversed the G2/M arrest partially (P < 0.01).

CONCLUSIONPaclitaxel and gemcitabine can enhance the growth suppression and apoptosis induced by paclitaxel in a synergistic way. They show great potential in the treatment of androgen-independent carcinoma of the prostate.

Antimetabolites, Antineoplastic ; pharmacology ; Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Deoxycytidine ; analogs & derivatives ; pharmacology ; Dose-Response Relationship, Drug ; Down-Regulation ; Drug Synergism ; Flow Cytometry ; Humans ; Male ; Paclitaxel ; pharmacology ; Prostatic Neoplasms ; pathology

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Expression and significance of granulocyte-macrophage colony-stimulating factor receptors in human prostate cancer.

Zhaohui CHEN ; Yu YANG ; Yajun XIAO ; Jun ZHAO

National Journal of Andrology.2004;10(9):655-657.

OBJECTIVETo study the expression and significance of granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors in human prostate cancer.

METHODSSP immunohistochemical method was employed to investigate the expression of alpha subunit of GM-CSF receptors in 48 cases of primary prostate cancer, 20 benign prostate hyperplasia samples and four kinds of cancer cell lines K562, PC-3M, HL-60 and MCF-7.

RESULTSThe total positive percentage of GM-CSF expression in prostate cancer was 79.2%. The positive percentages in the groups with Gleason score 2-4, 5-8, and 9-10 were 75%, 82.3% and 81.2% respectively. The four kinds of cancer cell lines had prominent GM-CSF receptor alpha subunit expression.

CONCLUSIONIt suggests that both hyperplastic and neoplastic prostate tissues are responsive to GM-CSF and the extensive expression of GM-CSF receptors is an important characteristic of prostate cancer.

Animals ; Blotting, Western ; Cell Line, Tumor ; HL-60 Cells ; Humans ; Immunohistochemistry ; K562 Cells ; Male ; Prostatic Hyperplasia ; metabolism ; Prostatic Neoplasms ; metabolism ; pathology ; Rabbits ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor ; biosynthesis

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Influence of Ureaplasma urealyticum infection on the sperm-egg binding associated molecule, sulfogalactosylglycerolipid.

Tingting WU ; Meige LU ; Yanqin HU ; Qiangsu GUO ; Chen XU

National Journal of Andrology.2004;10(9):651-654.

OBJECTIVETo study the influence of Ureaplasma urealyticum (Uu) infection on the sperm-egg binding associated molecule, sulfogalactosylglycerolipid (SGG).

METHODSEpididymal sperm was collected from adult mice. The sperm suspension was randomly divided into 4 groups: Uu group (coincubated with Uu suspension), medium group (coincubated with Uu medium), normal group and PRS group. The indirect immunofluorescence technique was used to localize SGG on the sperm membrane and to observe the influence of Uu on SGG.

RESULTSIn the epididymal sperm, SGG was localized to the head plasma membrane overlaying the acrosomal region. The SGG-positive rate of the sperm coincubated with Uu medium was 82.0%, while that of the sperm coincubated with Uu suspension was reduced to 39.0% (P = 0.001).

CONCLUSIONUu can adhere to the sperm surface. SGG might be a membrane receptor on the sperm surface for Uu infection of the mammalian male genital tract. The blockage of SGG by Uu might be one of the molecular mechanisms correlative to male infertility induced by Uu infection.

Animals ; Cell Membrane ; metabolism ; Galactolipids ; biosynthesis ; Infertility, Male ; etiology ; Male ; Mice ; Mice, Inbred BALB C ; Random Allocation ; Sperm-Ovum Interactions ; physiology ; Spermatozoa ; metabolism ; Ureaplasma Infections ; complications ; metabolism ; Ureaplasma urealyticum

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Effect of chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues.

Hengjun XIAO ; Jihong LIU ; Chunping YIN ; Tao WANG ; Jun CHEN ; Longchang FAN ; Zhi CHEN ; Zhangqun YE

National Journal of Andrology.2004;10(8):636-639.

OBJECTIVETo investigate the effects of chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues.

METHODSObservations were made on the relaxing effect of chuanxiongzine on phenylephrine (PE)-precontracted corpus cavernosum and cavernosal strips preincubated with L-NAME, and ODQ, using endothelium removal and experimental method of smooth muscle strips. The effect of chuanxiongzine on cAMP and cGMP levels in corpus cavernosum was measured by 125I radioimmunoassay.

RESULTSChuanxiongzine had concentration-dependent relaxing effect on the cavernosal muscle contraction induced by PE (EC50 1.58 x 10(-4) mol/L). This relaxing effect was partially antagonized by ODQ and not blocked by L-NAME or endothelium removal. In PE-precontracted cavernosal strips, chuanxiongzine induced relaxation accompanied with an increase in cAMP and cGMP levels.

CONCLUSIONChuanxiongzine was effective in relaxing cavernosal muscle precontracted by PE in vitro. And its relaxing effect may be enhanced by increasing cGMP and cAMP levels in the corpus cavernosum.

Animals ; Cyclic AMP ; metabolism ; Cyclic GMP ; metabolism ; Dose-Response Relationship, Drug ; In Vitro Techniques ; Male ; Muscle Relaxation ; drug effects ; Muscle, Smooth ; drug effects ; physiology ; Penis ; drug effects ; physiology ; Pyrazines ; pharmacology ; Rabbits

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Antioxidant vitamin and male reproduction.

Wanjian GU ; Xuejun SHANG ; Yufeng HUANG

National Journal of Andrology.2004;10(8):627-631.

Increased generation of ROS causes the lipid oxidation of the membrane of spermatozoa, but antioxidant vitamins play an important role in reproduction and help clear away ROS and protect the sperm membrane from lipid oxidation. This review focused on the effect of antioxidant vitamins on male reproduction and in the treatment of male infertility.
Animals ; Antioxidants ; pharmacology ; therapeutic use ; Ascorbic Acid ; pharmacology ; therapeutic use ; Humans ; Infertility, Male ; drug therapy ; Lipid Peroxidation ; drug effects ; Male ; Reactive Oxygen Species ; adverse effects ; Reproduction ; drug effects ; Vitamin A ; pharmacology ; therapeutic use ; Vitamin E ; pharmacology ; therapeutic use

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