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Antiapoptotic effects of rat hypertrophic cardiomyocytes induced with hypoxia/reoxygenation by dichloroacetate

Journal of Third Military Medical University.2002;0(12):-.

Objective To investigate the effect of hypoxia/reoxygenation on apoptosis of rat hypertrophic cardiomyocytes and the metabolic pathway changes of hypertrophic cardiomyocytes by dichloroacetate.Methods The isolated ventricular cardiomyocytes from neonatal Wistar rat were cultured and purified with differential attachment,then BrdU was added to reduce the rate of non-myocytes.The cultured cells were identified by morphology,spontaneous contraction and specific immunocytochemical stain.Hypertrophic cardiomyocyte model induced by angiotensin Ⅱ was set up and assessed by -Leu incorporation.The cultured media were replaced by low glucose DMEM before hypoxia.The cardiomyocytes were incubated at 37 ℃ in an air-tight incubator containing 92% N_(2),5% CO_(2),3% O_(2) for 24 h to simulate hypoxia,then under the condition of 23% O_(2),5% CO_(2) for 4 h to simulate reoxygenation.Apoptotic cells was evaluated by a modified TUNEL assay(DeadEnd~(TM) Colorimetric TUNEL).Results The apoptotic rate of cardiomyocytes increased with time prolongation of hypoxia/reoxygenation(H/R).The apoptotic cell model induced by H/R in rat hypertropic cardiomyocytes was established successfully.When the hypertropic cardiomyocytes were pretreated with Dichloroacetate,the apoptotic rate decreased in a dose-dependent manner.The metabolic pathway changes had effect on the apoptosis of rat hypertrophic cardiomyocytes.Conclusion By collagense digestion,it is convenient to obtain primary neonatal Wistar rat ventricular cardiomyocytes. This method is one of vital research tools in the fields of cardiac hypertrophy.Angiotensin Ⅱ could accelerate cellular proliferation,structural protein biosynthesis and cardiac hypertrophy.Dichloroacetate has the effect of antiapoptosis.

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Molecular mechanisms of Triple H therapy in the treatment of SAH-induced vasospasm

Ishiguro MASANORI ; B.puryear COREY ; Bisson ERICA ; Jewell RYAN ; J.nathan DAVID ; I.tranmer BRUCE ; C.wellman GEORGE

Journal of Third Military Medical University.2004;26(4):301-303.

Objective Cerebral artery vasospasm is a major cause of death and disability in patients experiencing subarachnoid hemorrhage (SAH). Vasospasm typically has been evaluated using angiography to examine narrowing of large diameter (>1 mm) cerebral arteries. Currently, little is known regarding the impact of SAH on small diameter (100～200 μm) cerebral arteries, which play an important role in the autoregulation of cerebral blood flow. The goal of the current study was to examine the influence of SAH on the pressure-diameter relationship of these small diameter blood vessels. Methods Small diameter cerebral arteries were obtained from a rabbit SAH model. Isolated artery segments were canulated and placed in a myograph chamber superfused with warmed, oxygenated, physiological saline solution. Diameter measurements were then recorded to step-wise increases in intravascular pressure. Results Cerebral arteries from SAH animals exhibited a significant increase in pressure-induced constrictions (myogenic tone) at intravascular pressures between 40 mmHg and 120 mmHg. At intravascular pressures above 120 mmHg, myogenic tone began to decrease and was abolished at pressures above 180 mmHg. Conclusion These data suggest that in the days following SAH, myogenic tone is enhanced in small diameter cerebral arteries subjected to physiological levels of intravascular pressure. However, supra-physiological intravascular pressures causes vasodilation, suggesting small diameter cerebral arteries may act as therapeutic targets of hypervolemia, hemodilution, and hypertension "Triple H therapy" used in the treatment of cerebral artery vasospasm.

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Preparation of Sodium salicylate-borate & Its Mycostatic Effect

Gtmlong TONG ; Zhenju WANG

Journal of Third Military Medical University.1983;0(03):-.

Sodium salicylate-borate is a complex salt easily prepared by the reaction of salicylic acid and borax at 40-50℃. A three-color point with Rf values of 0.72, 0.58, and 0.48 respectively was obtained on the thin layer chromatography. The maxium absorbent peak of this complex salt (20?g/ml) was at 300 nm. determined with the ultraviolet spectrophotometer scanner.The mycostatic effect of this complex salt varies with its concentrations. It is effective against the superficial infections with the concentration of over 1250 ?g/ml; and 2500?g/ml is necessary for the treatment of deep fungus infections.

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Intracranial Arachnoid Cyst

Cunsun ZHENG ; Xianrong WANG

Journal of Third Military Medical University.1983;0(03):-.

Most authors agree that arachnoid cyst accounts for about 1 % of all intracranial space-occupying lesions, but this lesion is more frequently found now-a-days as the application of cranial CT becomes more common, your cases of intracranial arachnoid cyst were reported with discussions on its etiolgy, pathogenesis, symptomatology, diagnosis, and treatment.In children, the presenting symptom is hydrocephalic manifestations; while in adults, it is headache with various focal symptoms depending on the locations of the cyst. There are certain indications for surgical therapy but direct excision is the treatment of choice in most cases. Surgical mortality should be seriously considered and not be neglected. No death occurred in our four cases.

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Clinical Application of Pseudomonas Aeruginosa Endotoxin Protein (EP) Vaccine in Pseudomonas Pneumonia

Defu JIANG

Journal of Third Military Medical University.1983;0(03):-.

pseudomonas pneumonia is a common and serious complication in neurosurge-ry. Its prognosis is poor and mortality high.The therapeutic effect of EP on 9 cases of pseudomonas pneumonia was studied from Sep. 1979 to Nov. 1981. The satisfactory results obtained were: the fever was lowered, the expectoration diminished, the antibody titer raised,the other symptoms and signs improved, and the bacterial culture converted negative.The results indicate that EP has some therapeutic value on pseudomonas pneumonia which is insensitive to most antibiotics.

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Effect of Pentobarbital on Monoamine Levels in Ischemic Brain

Xinfu ZHOU

Journal of Third Military Medical University.1983;0(03):-.

The effect of pentobarbital on cerebral protection and brain monoamine levels during cerebral ischemia were studied in 60 Mongolian gerbils. The animals were randomly divided into three groups. Pentobarbital(n = 20)and control(n = 20) groups which underwent left common carotid artery (CCA) ligation received 60 mg/kg of Pentobarbitalum natricum and equal volume of normal saline respectively at 30 min prior to operation. Sham-operated group (n=20)was treated as same as the control group with exception of not ligating CCA. The heurological deficits were evaluated and brain monoariiines measured at the third nour after operation.It was found that the stroke index was reduced, neurological deficits improved and brain norepinephrine(NE), dopamine(DA)and 5-hydroxytrytamine(5-HT) elevated to normal levels in pentobarbital-treated animals. There were significant negative correlations between stroke indexes and the levels of NE, DA and 5 -HT in ischemic brain (r = -0.485, -0.497 and 0.579 respectively, P

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Variations of Serum Lactate & Pyruvate Concentrations of Cor Pulmonale in Acute Exacerbation

Yali CONG

Journal of Third Military Medical University.1983;0(03):-.

The results of the arterial blood study in 30 patients of cor pulmonale in acute exacerbation were reported.The study included gas analysis, and dominations of serum lactate,pyruvate, potassium,sodium and chlorine.All the items were determined simultaneously.The data demonstrated that when the arterial oxygen tension of the patients with cor pulmonale in acute exacerbation dropped below 60mmHg, the blood lactate concentration was most likely to show a rise, which was related not only to hypoxemia but also to alkalosis.Observations of the changes in concentration of blood lactate and pyruvate in some patients led us to believe that these changes were associated with the seriousness of the disease. Regular measurement of blood lactate and pyruvate concentrations might be helpful to judge the condition of the patient. Persistent rise in blood lactate concentraion suggested poor prognosis.

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A Study of Senile VaginitisⅡ, Determinations of Vaginal Flora, Plasma Female Sex Hormones and Vaginal pH Value

Shirong HE

Journal of Third Military Medical University.1983;0(03):-.

Vaginal flora, plasma estradiol and progesterone levels and vaginal pH value were determined in senile vaginitis patients.In 36 senile vaginitis patients, mean number of species per culutre for aerobic organisms was 1.47, that for anaerobes was 1.22 and that for all organism (including both aerobic and anaerobic)was 2.69.14 species of aerobic organisms were isolated from the vaginal discharge of the patients. They were staphylococcus aureus (27-8), staphylococcus albus(19.4%), Klebsiella sp(l6.7%), E- cloacae (13.9%), beta-streptococcus ( 11.1% ), diphtheroids(11.1%), alpha-streptococcus(8.3%), B- Coli(8.3%), E. hafnia (8.3%), E. agglomerans(8.3%), streptococcus faecalls(5.6%), gamma-streptoco-ccus(2.8%), Gaffkya tetragena(2.8%) and Hemophilus(2.8% ). 9 species of anaerobic organisms were detected:peptococcus(36.1%), Bacterodies(including B-fragilis, B. melangenicus and other Bacteroides 27.8%), Veillonella(22.2%), peptostreptococcus(16.7%), Lactobacillus(13.9), pusobacterium(2.8%) and C. pergrigens(2.8%). Both aerobic and anaerobic organisms were common indigenous vaginal flora.The mean concentration of plasma estradiol in 34 patients was 42.44?28.40 pg/ml, Concentration of plasma progestrone was determined in 9 out of the 34 patients. The mean value was 0.87?0.63 ng/ml. The levels of these female sex hormones were lower than those found in postmenopausal women reported from Shanghai.The mean pH value was 7.18?0.89. Deviation of vaginal pH to alkalinity may be either the precipitating factor or the result of senile vaginitis.Factors concerned with the pathogenesis of senile vaginitis were discussed.

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Analysis of Mortality Rate of 3617 Burn Patients

Znaozhou LI

Journal of Third Military Medical University.1983;0(03):-.

From January 1959 to December 1981, 3617 burn patients were admitted to the First Teaching Hospital Burn Center. The ratio between male and female was 3:1. The highest incidence was found in the group under 5 years of age. It was most prevalent in summer season. Thermal injury was the major cause of burn accident. Most third degree burns were caused by hot or melted metals.In 5.97% of the cases the burn area exceeded morn than 70% body surface area. Total mortality rate was 7.52%. The incidence of complications was 26.90%. Septicemia was the most common one.The mortality rates of successive years and the probability among the burn area, age and mortality were calculated. Then the weighted linear regression equation was proposed.In analysing 254 cases who died in the course of treatment, it was found that the prevention and intensive treatment of shock, and the strict control of infection were the chief measures to reduc the incidece complications.It was emphasized that early diagnosis and treatment of respiratory tract burns and its pulmonary complications are crucial to reduc the mortality.

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Diagnosis & Treatment of Broncholithiasis -A Clinical Report of 5 cases

Zishan LONG

Journal of Third Military Medical University.1983;0(04):-.

This paper is to report the author's experience about the diagnosis and treatment of 6 cases of broncholithiasis.All the 6 patients had symptoms of recurrent cough and hemoptysis which was very severe and life-threatening in 4 cases.The diagnosis of broncholithiasis was rather difficult preoperatively. Conventional tomography played a very important role in verifying the existence of an intrabronchial stone. In 3 cases the diagnosis was established by tomography. Bronchoscopy was performed in 2 cases and broncholith was seen in one of them.One case recovered after his spontaneous expectorating the stone, while five cases were treated operatively; lobectomy was performed on 4 and pneumoectomy on one. One patient developed postoperative broncbopleural fistula and cure was only achieved after a further left upper lobectomy was performed. The final result of the five operated cases were excellent (having been followed up for 10 months to 19 years) .The pathological examination of the resected specimens revealed tuberculosis in two cases, silicosis in one and nonspecific chronic inflammation in the other two.Surgical management of broncholithiasis was briefly discussed and relevant literature reviewed.

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