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Changes of serum nitric oxide level after acute hepatic injury in mice and the effect of yugan capsule on it.

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():58-60.

OBJECTIVETo observe the changes of serum nitric oxide (NO) level after acute hepatic injury (AHI) in mice and the effect of Yugan capsule (YC) on it.

METHODSThe mouse AHI model was induced by carbon tetrachloride ( CC14 ) at different time points. After treatment with or without YC, serum nitric oxide ( NO) , anlanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected, and liver histopathologic injury was observed at different time points after AHI, respectively.

RESULTSSerum NO, ALT and AST in the model group escalated significantly with time going, while they decreased and liver histopathologic injury was improved in the YC groups.

CONCLUSIONThe serum levels of NO, ALT and AST increased,and liver histopathologic injury gradually aggravated with time going after CCl4 injection. Yugan capsule could decrease the levels of ALT, AST and NO.

Alanine Transaminase ; blood ; Animals ; Antioxidants ; therapeutic use ; Aspartate Aminotransferases ; blood ; Chemical and Drug Induced Liver Injury ; blood ; drug therapy ; enzymology ; Drugs, Chinese Herbal ; therapeutic use ; Mice ; Nitric Oxide ; blood ; Tablets

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Mechanism of hypothalamic effect in small intestine electro-activity of rats regulated by fructus aurantii immaturus.

Ting-ting SONG ; Qing-ying XUN ; Yi-quan WEI

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():55-58.

OBJECTIVETo observe the effect of Fructus Aurantii Immaturus (FAI) on the electro-activity of small intestines in rats, and evaluate the interrelations between the FAI regulating effect and choecystokinin (CCK) and somatostatin (SS).

METHODSMigrating myoelectric complex (MMC) cyclic period, the ratio of the active time to the cyclic period, and the number of the fast wave within the active time per minute were observed between FAI and the normal saline group by external alimentary canal electrodes; the CCK contents in dorsomedial hypothalamic nucleus (DMH), ventromedia hypothalamic nucleus (VMH), lateral hypothalamus area (LHA) and SS in VMH, LHA, paraventricular nucleus (PVN) by using immuno-chemistry technique and micro-image pattern quantitative analysis and scanning system.

RESULTSThe MMC cyclic period shortened, the ratio of the active time to the cyclic period increased and the number of the fast wave within the active time per minute increased in the FAI group, which showed significant difference from the normal saline group; CCK positive neurons were reduced in the areas of DMH, VMH and LHA, SS positive neurons were increased in the areas of VMH, LHA and PVN in the FAI gioup,which showed significant difference compared with the normal saline and the blank control group.

CONCLUSIONFAI can stimulate the electro-reactivity of small intestines. The stimulative effect of FAI might be related to CCK and SS in hypothamus.

Animals ; Central Nervous System Stimulants ; pharmacology ; Cholecystokinin ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Hypothalamus ; drug effects ; metabolism ; Intestine, Small ; drug effects ; physiology ; Myoelectric Complex, Migrating ; drug effects ; Rats ; Somatostatin ; metabolism

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Effect of banxia xiexin decoction and its components on coefficient of variability of slow wave electrogastric rhythm in rats with electrogastric dysrhythmia.

Yu-hang LI ; Qing-guo WANG ; Meng CHEN

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():53-55.

OBJECTIVETo observe the effect of Banxia Xiexin Decoction (BD) and its components in the rats with electrogastric dysrhythmia, explore the mechanism of BD' s "relieving distension and fullness, and dissolving lumps" and study BD' s compatibility regularity.

METHODSRat model of electrogastric dysrhythmia was established, the effects of BD and its components on the coefficient of variability of the model' s slow wave electrogastric rhythm were determined.

RESULTSCompared with the model group, BD and all the components groups had better effects on all the aspects (P <0. 05 ), Among which the group containing herbs with pungent and bitter flavour (Rhizoma Pinelliae, Rhizoma Zingiberis, Radix Scutellariae, Radix Berberidis julianae) was the best.

CONCLUSIONBD and all the components have the effects of rectifying electrogastric dysrhythmias in various degrees, and the herbs with pungent and bitter flavour are best. This study provides the experimental foundations for further understanding the mechanism of BD' s treating principle-using bitter drugs for purgation and pungent drugs for dispersion, normalizing the functional activities of qi.

Animals ; Digestive System ; drug effects ; physiopathology ; Electrodiagnosis ; Gastrointestinal Motility ; drug effects ; Medicine, Chinese Traditional ; Myoelectric Complex, Migrating ; drug effects ; Plant Extracts ; pharmacology ; Rats ; Stomach Diseases ; physiopathology

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Electromicroscopic study on effect of resolving phlegm of compound qianbei granule in mice.

Yan-Ping ZHANG ; Xue-dong AN ; Wen-jiang ZHANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():51-52.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Medicine, Chinese Traditional ; Mice ; Microscopy, Electron ; Sputum ; drug effects

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Effects of Ginkgo biloba extract on proliferation and apoptosis of T lymphocytes in vitro in rats with asthma.

Yi-jun TANG ; Yong-jian XU ; Zhen-xiang ZHANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():47-50.

OBJECTIVETo explore the partial therapeutic mechanism of Ginkgo Biloba extract (GBE) in treating asthma.

METHODSFourteen SD rats were randomly divided into two groups, 7 rats were sensitized as the asthmatic model group and the others taken as the healthy control group. T lymphocytes were isolated from peripheral blood mononuclear cells (PBMCs) of the rats, and were cultured in vitro with Ginkgolide B (BN-52021 group) or Ginkgo Biloba extract 761 (EGb761 group) in different concentrations or without any of them (control group). T lymphocytes proliferation in groups were measured by using MTT assay and the effect of BN-52021 on T lymphocytes apoptosis was analyzed by flow cytometry at various times.

RESULTSCompared with the control group, BN-52021 could significantly inhibit the proliferation of T lymphocytes in both healthy and asthmatic rats in vitro (P <0. 05). The effects were enhanced as the concentration increasing and the time prolonging, the effects to the latter were higher than those to the former, showing significant difference between them ( P <0.05 ). However, the effect of EGb761 was varied with the concentrations. EGb761 could promote T lymphocytes proliferation at low concentration but inhibit it at high concentration, there was a significant difference as compared with that in the control group ( all P < 0. 05). The apoptotic rate of T lymphocytes rose as the concentration of BN-52021 increasing (P < 0. 01).

CONCLUSIONGBE has different effects on T lymphocytes proliferation since the different ingredients and the concentrations in vitro, and it also has different effects between healthy and asthmatic rats. Ginkgolide B is the main active ingredient among them, it can not only inhibit T lymphocytes proliferation but also increase the apoptotic rate.

Animals ; Apoptosis ; drug effects ; Asthma ; drug therapy ; immunology ; pathology ; Cell Proliferation ; drug effects ; Ginkgo biloba ; Plant Extracts ; pharmacology ; therapeutic use ; Rats ; Rats, Sprague-Dawley ; T-Lymphocytes ; drug effects

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Effect of shuanglong capsule on content of leukotrienes compound of lung tissues in asthmatic rats.

Zhi-hui YANG ; Jian-jun ZHANG ; Lin-yuan WANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():43-46.

OBJECTIVETo observe the changing contents of leukotriene B4 ( LTB4 ), leukotriene C4 ( LTC4 ), and leukotriene D4 (LTD4 ) of lung tissue in asthmatic rats, and explore the effect of Shuanglong Capsule (SLC) on it.

METHODSSD rats were randomly divided into the nomal group, asthmatic model group, Dexamethasone group and the high, middle and low dose SLC groups. All rats except those in the normal group were sensitized by ovalbumin and challenged with the antigen, and the contents of LTB4, LTC4 and LTD4 in lung tissue of all the groups were measured by reverse phase-high performance liquid chromatography (RP-HPLC) and compared.

RESULTSThe levels of LTB4, LTC4, and LTD4 of asthmatic rats were significantly higher than those of rats in the normal group. Dexamethasone and SLC at the dose of 8. 27 g/kg or 4. 13 g/kg could significantly inhibit the production of leukotrienes of lung tissue in asthmatic rats (P <0.05).

CONCLUSIONSLC can significantly inhibit the formation of inflammatory medium LTs of lung tissue in asthmatic rats, it may be one of the key mechanisms of SLC in anti-asthma and anti-inflammatory action.

Animals ; Anti-Asthmatic Agents ; pharmacology ; Asthma ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Leukotriene B4 ; metabolism ; Leukotriene C4 ; metabolism ; Leukotriene D4 ; metabolism ; Leukotrienes ; metabolism ; Lung ; drug effects ; metabolism ; Rats ; Rats, Sprague-Dawley ; Tablets

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Study on the pharmacological mechanism of analgesic effect of injection stauntoniae.

Su CHEN ; Xiang-ming LIU ; Fan ZHANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():39-43.

OBJECTIVETo study the effects of Injection Stauntoniae (IS) on voltage-gated sodium currents in dorsal root ganglion neurons and analyze its pharmacological mechanism of blocking the nerve conduction and anal gesic action.

METHODSWhole-cell patch-clamp recordings were performed in acutely isolated rat dorsal root ganglion neurons and the effects of 10% , 25% and 50 % IS on voltage-gated sodium currents were observed.

RESULTSIS inhibited the peak sodium currents in dorsal root ganglion neurons in a dose-dependent way and affected the activation and inactivation process of the channels.

CONCLUSIONThe analgesic effect of IS was presumably caused by modulation of voltage-gated sodium channels in primary sensory neurons besides structure destruction of myelin sheath and axon membrane.

Analgesics ; administration & dosage ; pharmacology ; Animals ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; Ferns ; Ganglia, Spinal ; drug effects ; metabolism ; Injections ; Neurons ; drug effects ; metabolism ; Rats ; Sodium Channels ; drug effects

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Receptor mechanism of anti-convulsant effect of caoguo zhimu decoction.

Li-ping ZHANG ; Li WANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():36-38.

OBJECTIVETo study the receptor mechanism of anti-convulsant effect of Caoguo Zhimu Decoction (CZD).

METHODSSD rats in traditional Chinese medicinal (TCM) group were given CZD by gastrogavage for 24 days, while those in the control group were given normal saline of the same volume. The difference of convulsant threshold was compared between the TCM group and the control group induced by pentylenetetrazole. Changes of gamma-aminobutyric acid A receptor (GABAAR-alpha1 ) and N-methyl-D-aspartate receptor-NR1 (NMDAR-NR1 ) protein expression in hippocampus were quantitatively and orientatively measured by Western blotting and immunohistochemistry method. The changes of the two receptors were compared by receptor ligand binding assay.

RESULTSThe expression of GABAAR-alpha1 subunit in the TCM group was higher, but expression of NR1 subunit protein showed no significant difference when compared with those of the control group. The binding properties of GABAA receptor in the TCM group was obviously raised, but there was no obvious change in NMDA receptor.

CONCLUSIONThe anticonvulsant effect of CZD may be achieved by raising expression of GABAA R-alpha1 subunit, and enhancing its binding with ligand.

Animals ; Anticonvulsants ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Hippocampus ; metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA-A ; drug effects ; metabolism ; Receptors, N-Methyl-D-Aspartate ; drug effects ; metabolism

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Protective effect of renshen yangron decoction on morphology and structure of mitochondria in brain and liver of subacute senile mice.

Hong DENG ; Fang TANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():33-35.

OBJECTIVETo study the effect of Renshen Yangrong decoction (RYD) in protecting morphology and structure of mitochondria in brain and liver of D-galactose induced subacute senile mice.

METHODSForty ICR mice were randomly divided into the youth control group, the model group, the high, middle and low-dose RYD groups. The ultra-structure changes of mitochondria were observed by electronic microscope, the specific surface area, body density, numerical density on area, deltammit area and ridge count of mitochondria were quantitatively analyzed with medical image analyzer.

RESULTSIn the model group, the numbers of mitochondria decreased, mitochondria swelled and significantly expanded, ridge was fragmented, malformed and dissolved even vanished. Those manifestations were coincided with the morphological changes of mitochondria in senile mice. In the different dose of RYD groups, the numbers of mitochondrion increased, mitochondrion tumid were significantly improved, senile degenerative changes of ridge and intima were obviously alleviated in a dose-dependent manner.

CONCLUSIONRYD can anti-age by improving the changes of mitochondria structure of liver and brain accompanied with ageing.

Aging ; drug effects ; Animals ; Brain ; drug effects ; ultrastructure ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Liver ; drug effects ; ultrastructure ; Mice ; Mice, Inbred ICR ; Mitochondria ; drug effects ; ultrastructure ; Panax ; Protective Agents ; pharmacology ; therapeutic use

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Protective effect of renshen yangrong decoction on membrane fluidity of mitochondrion in brain and liver of subacute senile mice.

Hong DENG ; Fang TANG

Chinese Journal of Integrated Traditional and Western Medicine.2006;26 Suppl():30-33.

OBJECTIVETo study the effect of Renshen Yangrong decoction (RYD) in protecting membrane fluidity of mitochondrion in brain and liver of D-galactose induced subacute senile mice.

METHODSForty ICR mice were randomly divided into the youth control group, the model group, the high, middle and low-dose RYD groups. The membrane fluidity of mitochondrion in liver and brain cell was measured, and the malondialdehyde (MDA) content of mitochondrion was determined and analyzed.

RESULTSIn the model group, the membrane fluidity of mitochondrion in hepatic and cerebral cells significantly decreased, and the MDA concentration of mitochondrion increased. RYD could significantly reduce the MDA concentration of mitochondrion, markedly increase the membrane fluidity of mitochondrion in a dose-dependent manner.

CONCLUSIONRYD plays a role in anti-aging through protecting the membrane fluidity of mitochondrion in hepatic and cerebral cells and decreasing the MDA concentration of mitochondrion changing with ageing.

Aging ; drug effects ; physiology ; Animals ; Brain ; drug effects ; metabolism ; physiology ; Drugs, Chinese Herbal ; pharmacology ; Malondialdehyde ; metabolism ; Medicine, Chinese Traditional ; Membrane Fluidity ; drug effects ; Mice ; Mice, Inbred ICR ; Mitochondria ; drug effects ; metabolism ; physiology ; Mitochondria, Liver ; drug effects ; metabolism ; physiology ; Panax ; Protective Agents ; pharmacology

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