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Journal of Practical Stomatology

1985  to  Present  ISSN: 1001-3733

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The influence of sucrose concentration environment on the inhibition of Streptococcus oligofermentans on Streptococcus mutans

Mengci LI ; Fei WU ; Xiaobin YANG ; Lei CHU ; Ying LIU ; Ligeng WU

Journal of Practical Stomatology.2014;(2):156-160. doi:10.3969/j.issn.1001-3733.2014.02.02

Objective:To investigate the inhibition of Streptococcus oligofermentans(So)on Streptococcus mutans(Sm)and the hydro-gen peroxide(H2 O2 )producibility by So under different sucrose concentration environment.Methods:The inhibition of So on Sm was observed by plating method under different sucrose concentration environment.The initial synthesis rates and production of H2 O2 by So were determined by 4-aminoantipyine-horseradish peroxidase method.Results:Under 500 mmol/L of H2 O2 ,the inhibition of So on Sm was not observed.Under the other sucrose concentration environment,the inhibition of So on Sm was as following:50 mmol/L >0 mmol/L and 1 mmol/L(P <0.05);the initial synthesis rates of H2 O2 by So under different sucrose concentrations were as following:50 mmol/L >0 mmol/L and 1 mmol/L >500 mmol/L(P <0.05);the total production of H2 O2 by So:0 mmol/L and 1 mmol/L >50 mmol/L >500 mmol/L(P <0.05).When So was inoculated before Sm,the inhibition of So on Sm was stronger than that when the two species were inoculated at the same time.Conclusion:The capability of the inhibition of So on Sm and the production of H2 O2 by So are influenced by sucrose concentration.

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The effects of different titanium alloy surface topograph on the biological character of fibroblasts

Wentian WANG ; Weiyan MENG ; Yanmin ZHOU ; Qing CAI ; Shuyan WANG ; Jingjing WANG ; Tongbin LIU

Journal of Practical Stomatology.2014;(2):151-155. doi:10.3969/j.issn.1001-3733.2014.02.01

Objective:To compare the effect of three different titanium alloy surface topographies on the biological character fibro-blasts.Methods:Three different titanium alloy Ti6Al4V surface topographies were created by machining(M),direct current electro-chemical etching(DC)and alternating current electrochemical etching(AC)respectively,and standard sized samples were prepared. The surface topographies were observed field emission scanning electron microscopy(FE-SEM).Mouse L929 fibroblasts were cultured on different surfaces.The attachment of cells was examined by acridine orange staining and observed under fluorescent microscope.The proliferation of cells was measured using MTT assay.Results:Tubles with the diamete of 5 -20 μm were observed on the surface of the sample of group DC,and more evenly distributed tubles on that of group AC.Micro-sulci were observed and no microtuble was found on the sample surface of group M.L929 cells were attached on the surface of all the samples in the 3 groups.More adhered and better strached cells were observed on AC treated surface.The proliferation of fibroblasts on AC and DC surfaces was superior to that on M treated surface and at the 3 -5 day the proliferation of fibroblasts on the AC surface was higher than that on DC treated surface.Con-clusion:AC treated surface with the micro-submicro-nano hierarchical topography of titanium alloy has a superior biocompatibility and can promote the early attachment and proliferation of the fibroblasts.

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Hemisection for the treatment of maxillary lateral incisor fused with a supernumerary teeth---A case report

Yankai WANG ; Lei GAO ; Liqiang SHI ; Yanan SUN ; Lian WU

Journal of Practical Stomatology.2014;(2):284-286.

Fused teeth is defined as the union of enamel and dentin of two or more teeth germs,yielding a single tooth during tooth develop-ment.Their treatment is complex and usually need multidisciplinary approaches.This report presents a case of fused teeth treated by he-misection and discusses the clinical features and treatment options.

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Rapid maxillary expansion in the treatment of obstructive sleep apnea syndrome:A case report

Jianjie LIU

Journal of Practical Stomatology.2014;(2):282-284.

1 case of OSAS was treated by valuating rapid maxillary expansion,sleep monitoring indicated that the device reduced nasal re-sistance and improved respiratory function of the patient.

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Clinical application of biosplint splinting ribbon in the stabilization of the mobile teeth and flowable restorative for the replacement of missed anterior teeth in patients with severe periodontitis

Yan SUN ; Yuanyuan KANG ; Ying ZHANG ; Yan WEN

Journal of Practical Stomatology.2014;(2):279-282.

Biosplint splinting ribbons and flowable restorative were used to stabilize the mobile anterior teeth and replace the missed teeth with in 30 patients severe periodontitis.1 and 2 years after treatment the effective ratio was 86.7% and 83.3% respectively(P >0.05), probing depth and attchment loss were decreased(P <0.05).

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Significances of epithelial-mesenchymal transition and TGF-β1 expression in oral carcinoma

Xia WANG ; Shuhua WU ; Peiyuan WANG ; Yuhong ZHU

Journal of Practical Stomatology.2014;(2):277-279.

Immunohisto-chemical S-P method was used to examine the expression of E-cadherin,Vimentin and TGF-β1 in 54 cases oral carcinoma and 10 cases of normal oral mucosa.The positive rates of the expression of E-cadherin,Vimentin and TGF-β1 were 55.6%, 33.33% and 59.3%,respectively in oral carcinoma group(vs normal oral mucosa,P <0.05).The expression of TGF-β1 was correlated with lymph node metastasis,and showed significant positive correlation with Vimentin.Epithelial-mesenchymal transition may promote inva-sion and metastasis of oral carcinoma,and TGF-β1 promotes EMT.

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SOX-2 expression in ameloblastoma and odontogenic cyst

Wenhua YANG ; Baolian ZHAO

Journal of Practical Stomatology.2014;(2):275-277.

Immunohistochemistry examination showed that SOX-2 was expressed in all 23 cases of odontogenic cyst and in 2 cases of poly-cystic ameloblastoma.

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The factors of nickel titanium instrument seperation in posterior tooth root canal therapy

Jianyong DING

Journal of Practical Stomatology.2014;(2):257-259. doi:10.3969/j.issn.1001-3733.2014.02.025

Objective:To study the factors of nickel titanium instrument(NTI)seperation in posterior tooth root canal therapy.Meth-ods:NTIs were used in 1 498 patients with 1 562 involved posterio teeth and 3 518 root canals for RCT from Jun.2009 to May.2012. The relation of instrument seperation(IS)with gender,age,tooth position,working years of the dentists,bending degree of the in-volved tooth canal and the NTI form was analyzed statistically.Results:68 out off 1 198 applied NTIs(5.68%)were seperated in cal-nal preparation.Single factor analysis showed that IS incidence was not statistically different between male and femal(χ2 =0.013,P >0.05),it was statistically different among the patient ages(χ2 =-354.258,P <0.05),between molar and premolar(χ2 =-201.344,P <0.05),among working years of the dentists(χ2 =13.763,P <0.05),bending degree of involved teeth(χ2 =22.414,P <0.05),times of NTI uses(χ2 =16.807,P <0.05),taper of NTIs(χ2 =18.283,P <0.05)and diameter of NTIs(χ2 =21.312,P <0.05).Logistic analysis showed that age,tooth position,working years of dentists,bending degree of involved tooth canal,frequency of NTIs applica-tion and NTI form were independent risk factors for NTI seperation.Conclusion:Dentists should take appropriate technique and stand-ard operation sequence to reduce the incidence of nickel titanium instrument seperation.

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The impact of smoking on C-reactive protein level in gingival crevicular fluid before and after initial treat-ment of patients with periodontitis

Yindi AN ; Li ZHANG ; Zheng ZHANG ; Ning MA

Journal of Practical Stomatology.2014;(2):254-256. doi:10.3969/j.issn.1001-3733.2014.02.024

Objective:To study the impact of smoking on C-reactive protein(CRP)concentration in gingival crevicular fluid(GCF) before and after initial treatment.Methods:18 smokers and 18 non-smokers with moderate or severe periodontal disease were recrui-ted into this study.The clinical indexes of periodontal examination of the patients were recorded,GCF samples of the patients were collected,CRP level in the samples was measured by radioimmunoassay balance method before and 4 weeks after initial treatment. Results:4 weeks after initial treatment,the clinical indexes and CRP concentration in GCF of the smoking group and non-smoking group were significantly lower than those before treatment(P <0.05),the changes of the smoking group were less than those of the non-smoking group(P <0.05).Conclusion:Smoking is an unfavorable factor of the initial periodontal therapy.

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The effects of N2 O inhalation sedation in the management of dental fear for dental treatments of children

Hao CHEN ; Hong LUO ; Guanglei HUANG ; Ke ZHAO

Journal of Practical Stomatology.2014;(2):251-253. doi:10.3969/j.issn.1001-3733.2014.02.023

Objective:To study the effects of nitrous oxide(N2 O)sedation in the management of dental fear(DF)for dental treat-ments of children.Methods:66 cases of pediatric patients(aged 6 to 14 years)were given N2 O sedation for dental treatments.The heart rate(HR)and oxygen saturation(SpO2 )were measured before and after N2 O inhalation.N2 O effective concentration was eval-uated by Ramsay sedation score and Houpt behavior score.Results:The effective concentration of N2 O sedation was 25% -70%(49.6% ±12.1%),the maximum endurance concentration 35%-70% (56.4% ±10.1%).The Ramsy scores of N2 O sedation was (2.3 ±0.6).After N2 O inhalation,all patients could receive verbal demand during the treatments.The HR decreased(P <0. 05)and the Houpt behavior score increased(P <0.01).Before and after N2 O inhalation SpO2 had no significant difference(P >0.05).Conclusion:N2 O inhalation at 25% -70% is safe and effective in the management of DF for dental treatments of children.

Country

China

Publisher

第四军医大学口腔医学院

ElectronicLinks

http://sykqyxzz.paperopen.com

Editor-in-chief

E-mail

j-pr-s@fmmu.edu.cn

Abbreviation

Journal of Practical Stomatology

Vernacular Journal Title

实用口腔医学杂志

ISSN

1001-3733

EISSN

Year Approved

2007

Current Indexing Status

Currently Indexed

Start Year

1985

Description

历史沿革【现用刊名:实用口腔医学杂志;创刊时间:1985】,该刊被以下数据库收录【CA 化学文摘(美)(2009);CBST 科学技术文献速报(日)(2009);Pж(AJ) 文摘杂志(俄)(2009);中国科学引文数据库(CSCD—2008)】,核心期刊【中文核心期刊(2008);中文核心期刊(2004);中文核心期刊(2000);中文核心期刊(1996);中文核心期刊(1992)】。

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