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Effect of occlusal splint oa electromyogram of lateral pterygoid muscie dysfunction.

Journal of Practical Stomatology.1995;0(04):-.

The electromyogram(EMG) of lateral pterygoid muscle (LP) in 43 patients with LP dysfunction was measured before, when and after the use of occlusal splint. The postural and clenching activity of superior LP decreased dramatically when patients were wearing the occlusal splint. Normal EMG was observed in patients with normal arthrograra when the symptoms and signs were eliminated or reduced by occlusal splint. The EMG of dysfunctional LP in some patients with anteriorly displaced disk recovered to that with normal function as the incoordinated condyle -disk rektion was corrected. However, LP dysfunction was still present in most patients with irreversible anterior disk displacement and disk perforation after splint therapy.

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A 8-year follow-up observation of fraternal derangements of the temporomandibular joint treated with anterior repositioning splint

Yin XINMIN

Journal of Practical Stomatology.1995;0(04):-.

90 patients of internal derangement of the TMJ were treated with anterior repositioning splint designed by the authors and were followed up for 8 years. Anterior disc displacement of the TMJ with reciprocal clicking can be successfully cured with the new type splint. The total successful therapeutic rate was 93. 3%. There was no recurrence of clicking when the splint was removed. The original intercus-pal position of mandible did not change and there was no need to make occlusal reconstruction for the patients.

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Carcinoma of salivary glands-a clinical analysis of 150 cases.

Yang JUN

Journal of Practical Stomatology.1995;0(04):-.

150 cases with carcinoma of salivary gknds were analysed. The tumors of 61 cases were located in parotid, 14 in submaxillary, 5 in sublingual and 72 in minor salivary gknds. 49 cases were pathologically diagnosed as malignant mixed tumor ,25 adenocarcino- ma, 24 adenoid cystic carcinoma,22 mucoepidermoid carcinoma,22 acinic cell carcinoma, 5 squamous cell carcinoma , 2 papillary cystadenocarcinoma, 1 undif-ferentiated carcinoma. The 3,5 and 10 year survival rates of these 150 patients were 80. 7% ,69. 4%. 52. 1% respectively. The prognosis of acinic cell carcinoma and mucoepidermoid carcinoma was much better than that of squamous cell carcinoma, adenocarcino-ma and undifferentiated carcinoma. According to the location of the tumors, the survival rates of the patients with the tumors in minor salivary glands and parotid glands were the highest and those in sublin-gual glands and submaxillary glands were the lowest . Postoperative radiotherapy and chemotherapy may increase the survival rate of the patients with carcinoma of salivary glands. The overall recurrent rate was 27. 6% ,the neck lymph node metastasis rate was 9.2%.

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Experimental study of deep-frozen on the decrease of antigenicity of allogenic bone.

Xinghua FENG

Journal of Practical Stomatology.1995;0(04):-.

Cellular immunological reaction of the subjects after transplantation of deep-frozen (at -196℃) al-logenic bone grafts was studied using the sensitive LDH - release assay of morden immunological technique. The antigenicity of allogenic bone was definitely decreased after deep-freezing treatment and related to the time of treatment. In the experimental models the antigenicity of the deep-frozen allogenic bone was reduced to the same level as that of isogenic bone when it had been frozen at-196℃ for 7 days.

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Relationship between peridontitis and prostaglandin E 2 content in gingiva and alveolar bone in guinea pigs by radioimmunoassay

Tielou CHEN

Journal of Practical Stomatology.1996;0(02):-.

20 guinea pigs were equally divided into two groups. Periodontitis was formed with silk thread suture and high sugar diet in one group; the other group was used as the control. The prostaglandin E 2 (PGE 2) in gingiva and in alveolar bone was measured with radioimmunoassay(RIA). PGE 2 level in gingiva in both groups was higher than that in alveolar bone. In the control group PGE 2 in gingiva (394.87?118.27) was 2.1 fold as much as that in alveolar bone(182.91?33.29)( P

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Fabrication of tissue engineered bone by seeding osteoblasts into titanium coral scaffolds

Fulin CHEN ; Tianqiu MAO ; Guicong DING

Journal of Practical Stomatology.2001;0(01):-.

砄bjective:To fabricate bone tissue that has similar structural and mechanical characters with normal bone.Methods: Titanium meshes were molded into the shape of column in the length of 12 mm and in the diameter of 8 mm. The column was filled with natural coral granduls.4?10 7 marrow derived osteoblasts in 200 ?l cell culture medium were seeded into each of five scaffolds and incubated in vitro for 2 d to ensure that cells adhere well on the scaffolds. Then the scaffolds were implanted subcutaneously into the back of nude mice. Two months after implantation, the animals were sacrificed and the implanted materials were investigated by gross specimen inspection, X ray examination and histological observation. Results:2 months after in vivo incubation, the newly formed tissue was red and had the gross appearance of bone, and kept the original shape of column. Titanium mesh situated in the surface area. X ray examination showed that large amount of new bone formed in the scaffolds, there was no space between new bone and titanium mesh. Most of coral granduls had been absorbed. Histological observation demonstrated that in the surface area, new bone integrated well with titanium mesh and was enforced by titanium mesh(like cortical bone), and in the middle area large amount of lamellar bone formed.Conclusion: Newly formed bone in this experiment has similar structural with normal cortical bone.

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Expression of nm23-H1 gene in oral squamous cell carcinoma and its relationship with metastasis to neck lymph nodes

Gang LIU ; Jinrong LI ; Yaojun DONG

Journal of Practical Stomatology.2001;0(01):-.

砄bjective:To investigate the variation of nm23 H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23 H1 expression and OSCC metastasis to neck lymph nodes.Methods: nm23 H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis; nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results: All samples showed no change in nm23 H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23 H1 mRNA,however,only 5 were found no nm23 H1 mRNA in 21 cases without metastasis,positive rate of nm23 H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23 H1 gene showed significant negative relationship with neck lymph nodes metastasis ( P

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Application of carboplatin-activiated carbon particle delivery system in the treatment of oral carcinoma and its cervical metastasis

Cunping QIU ; Changmei WANG ; Yuming WEN

Journal of Practical Stomatology.2001;0(01):-.

?Objective: To estimate the effect of carboplatin activiated carbon particle delivery system (CP CH) in the treatment of oral carcinoma and its cervical lymph node metastasis. Methods: CP CH was prepared by absorption of carboplatin with activated carbon particles and injected into the submucosa around the tumors 72 h before operation in 6 patients with tongue or buccal squamous cell carcinoma (SCC) and metastasis in cervical lymph nodes,then extensive tumorectomy with neck dissection was conducted. The specimens of lymph nodes,primary carcinoma and blood were analyzed by microscope and plasma emitting spectrometry. Results:60 out of 70 (83.33%) lymph nodes obtained from the CP CH treated cases were found to be stained black by carboplatin.The carboplatin concentrations in lymph nodes and blood were (23.36 ?2.58) ?g/g and (1.76?0.32) ?g/ml respectively.Necrosis and degeneration were observed in SCC tissue.Conclusion:CH CP may be effective in the treatment of oral SCC with cervical lymph metastasis by providing high concertration and long lasting carboplatin in the lessions.

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An immunohistochemical study of TGF-?1 in monkey mandibular condyle following gradually induced occlusal disorder

Jinwu CHEN ; Meiqing WANG ; Xiufang YAO

Journal of Practical Stomatology.1996;0(02):-.

砄bjective:To elucidate the effects of gradually induced occlusal disorder on the expression of TGF ?1 in temporomandibular condyle of monkey. Methods:In a monkey after extraction of right upper first molar and left lower first molar, a fixed orthodontic appliance was used to cause the adjacent teeth inclined to the space and to occlude with opposite in coordinately. Another monkey was used as the control.8 months later, the animals were sacrificed and bilateral temporomandibular condyles were examined with histologic and immunohistochemical methods.Results:In the animal with teeth extraction, degenerative changes were found in anterior medial part of condyles, the thickness of condylar cartilage decreased significantly, especially in the anterior parts of medial and central thirds, and also the intermediate part of medial third ( P

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Mineralization in dental germs observed with modified Mallory's trichrome staining

Lintian YUAN ; Lingying WEN ; Weizhong JIANG

Journal of Practical Stomatology.1996;0(02):-.

Objective:To observe the dynamic process of the development of dentin and enamel during their mineralization period. Methods: Dental germs in maxillae and mandibulae were obtained from spontaneously aborted 21 weeks and 32 weeks fetus. The samples were prepared and stained with modified Mallory's trichrome staining and observed under light microscope. Results: Different mineralized layers in different period of development appeared different color variation in the dental germs. Conciusion: Modified Mallory's trichrome staining method may be used to study the mineralization of dental germ.

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