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Recombinant adenovirus carrying tissue inhibitor of metalloproteinase-3 gene regulates the matrix of rabbit intervertebral disc in vivo

Liming XIONG ; Bing GUO ; Zengwu SHAO ; Shuhua YANG ; Mao XIE ; Hezhong WANG

Orthopedic Journal of China.2009;17(5):356-360.

To investigate the influence of recombinant adenovirus carrying tissue inhibitor of metalloproteinase-3 (RAdTIMP-3) on the main compositions of rabbits intervertebral discs and to assess its potential in treatment for intervertebral disc degeneration.[Method]RadTIMP-3 and empty adenovims vector with Lac-Z gene (Rad66) was propagated in 293 Cells and was purified, identified and tittered. Thirty Japanese white rabbits were randomly divided into 5 groups. And 25 μl of various reagents were injected to the L4、5 and L5、6 intervertebral discs of the rabbits as follows:normal saline in group 1, 1.0×1010 OPU/ml of RAd66 in Group 2, and 1.0×1010 OPU/ml of RAdTIMP-3 in group 3, 4 and 5. The intervertebral discs of each group were collected after 2, 2, 1, 2 and 4 weeks after injection respectively.Then X-gal staining, And Group 1, RT-PCR for TIMP-3 and aggrecan core protein,TUNEL staining, immunohistochemical staining for TIMP-3 and type I! Collagen and Safranin O-Fast green staining was carried out to assess the effects of RadTIMP-3 transfection.[Result](1)concentration of RAdTIMP-3 reached 1.9×1012 OPU/ml after propagation and purification. (2)RT-PCR shows that the expression of TIMP-3 was significantly raised in group 3, 4, 5, as compared with group 1 or 2. And the expression of core protein gene in group 3, 4, 5 increased slightly than in group 1 and 2. (3) TUNEL staining revealed that there was not significant difference between the positive-staining rates of any two of the groups. (4)TIMP-3 staining exhibited an obvious increase of positive-staining rates in group 3, 4 and 5 as compared with groupi or 2. The staining density of Safranin O-Fast Green staining and immunohistochemical staining for type II collagen of group 5 was obviously higher than that of group 1 or 2.[Conclusion]RAdTIMP-3 can express widely and safely in rabbit intervertebral discs, and improve the quantity and quality of matrix. It has the potential to be used in treatment for intervertabral disc degeneration.

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Minimally invasive nucleoplasty combined with Oxygen-Ozone therapy for the treatment of lumbar disc herniation

Anli YANG ; Huaming XUE ; Feng CAI ; Liang LIU

Orthopedic Journal of China.2008;16(23):1818-1821.

[Objective]To explore the mechanism of nucleoplasty combined with oxygen-ozone for the treatment of lumbar disc herniation，and to evaluate its therapeutic effects.[Method]From June 2005 to June 2006，62 patients with lumbar disc herniation were treated by nueleoplasty combined with oxygen-ozone，34 were male and 28 were female，ranging in age from 32 to 76 years，with an average of 53.4 years.The course of this disease ranged from 3 to 56 months，meanly 1.3 years.Thirteen patients had the herniation at L4.5 and L3，4，thirty two patients at L4、5，eight patients at L4、5 and L5S1，and nine patients at L5S1.The curative effect was assessed by using a modified MacNab method.[Result]After 25～35 months'follow-up，there were no servious complications in all 62 cases.According to modified MacNab assessment，50 cases(80.7%)achieved excellent outcomes，8 cases(12.9%)good，2 cases(3.2%)fair and 2 cases(3.2%)poor.The excellent and good rate was 93.5%.[Conclusion]The micro-invasive technique of nueleoplasty combined with oxygen-ozone is effective in the treatment of lumbar disc herniation，and it has maximized the clinical effect and minimized the local damage：it also expanded adaptive cases with lumbar disc herniation and enlarged the applicable scope of minimally invasive technique in treating lumbar disc hemiation.

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Giant cell-rich osteosarcoma of the right humerus:case report

Wende XIAO

Orthopedic Journal of China.2008;16(21):1664-1665.

Giant cell-rich osteosarcoma is a rare osteosarcoma and easily regarded as giant cell tumor of bone by pathematology.Because two deseases have different therapies,misdiagnosis frequendy affect the operator to select suitable surgical procedures.We described the cage of a patient with giant cell-rich osteosarcoma,non vascularised fibular grafts were used as substitutes for repairing and reconstructing caput humeralis and shaft of humerus after resection for bone tumors on proximal humerus,part functions were remained after the operation.

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Results of total knee arthroplasty after high tibial osteotomy

Rajgopal ASHOK

Orthopedic Journal of China.2008;16(17):1319-1321.

[Objective] High tibial osteotomy is a well accepted procedure for medial compartment osteoarthritis of knees.Over time its results deteriorate and some patients were reoperated with a total knee arthroplasty.To evaluate the results of total knee arthroplasty after a high tibial osteotomy.[Method] Thirty-eight knees were evaluated retrospectively for the results of a total knee arthroplasty after a high tibial osteotomy.They were followed up for an average of 8 years and the mid terra results assessed.[Result] The average Hospital for special surgery score increased to 86.5 points from 39 points pre-operatively.There were 87% excellent and good results.[Conclusion] Mid term results of total knee arthroplasty after hish tibial osteotomy are excellent and are comparable to those without a tibial osteotomy.

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Diagnosis of osteoporosis by vertebral bone mineral density measurement with quantitative computed tomography

Ningjiang SHEN ; Yibo LI ; Wenqing CHEN ; Guanghong LIU

Orthopedic Journal of China.2008;16(11):826-828.

[Objective] To evaluate the clinical value of diagnosing osteoporosis with vertebra bone mineral density(BMD) by measurement with quantitative computed tomography (QCT) . [ Method] BMD in of lumbar vertebrae (L<,1～4>)were measured by QCT in 53 healthy middleaged persons or elderly persons (group A) and 68 osteoporosis patients ( groupB) . [Result] BMD in group A declined obviously as age increasing with significant differences (P<0. 01) . In group B,all 68 patients showed osteoporosis according to the diagnostic standard of BMD≤x -2. OSD. Sixty patients showed osteoporosis ifthe diagnostic standard was set to BMD ≤ x -2. 5SD. The BMD values of different age group was lower than the bone quantityblock values. [Conclusion] As a method for diagnosing osteoporosis, QCT has advantages of high sensitation, being precise,being reproducible and is easy for applying. It would be more practical and could decrease mis - diagnosing if the diagnosticstandard was BMD≤x-2.OSD.

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Determination of contact force at facet joint with different sagittal orientation under shearing loads:a finite element analysis

Yaosheng LIU ; Qixin CHEN ; Shubin LIU

Orthopedic Journal of China.2008;16(10):748-752.

[Objective] The efleet of sagittal orientations of articular facet on the contact force and contact regions of a lumbar spine segment was evaluated.[Methods] [Results]Three-dimensional geometrical and mechanical accurate finite element models of the lumbar L4、5 motion segment representing three sagittal-orientations articular facet were generated and invalidated by an effective CAD method.Contact force and contact regions of the articular facets of three finite element models were predicted under the pure shearing loads.[Result] Under anterior shear condition,the contact force on the facet decreased with the increase of sagittal 0rientation of articular facet.however.in posterior shear load,the contact force on the facet increased with the increase of sagittal orientation.Meanwhile,under both pure shear loading,the percentages of the sagittal and the transversal components are increased with the dectease of the sagittal angle.However,under the salne load condition,there are no difference between the contact regions of the three models with different facet joint angle.[Conclusion]The spatial orientation and geometric forms of the coronal facet articular surfaces are more effective in restricting motion in transversal and sagittal planes while assuming a minor role in resisting axial force or motion than sagittal facet articular surface.It Was presumed that anterior shear force play a more prominent contribution on the degeneration of the facet joint with coronal articular surface compared with posterior shear force.

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Structural augmentation with biomaterial loaded allograft threaded cage for the treatment of femoral head osteonecrosis surgical technique

Shuhua YANG ; Xinghuo WU ; Cao YANG ; Weihua XU ; Jin LI ; Shunan YE

Orthopedic Journal of China.2008;16(8):594-598.

[Objective]To introduce the surgical technique and its rationale and to evaluate the early and mid-term clinical outcome of routine core decompression and insertion of a biomaterial loaded allograft threaded cage(ATC)in the treatment of necrotic femoral head．[Methods]Seventy-six patients(78 hips)with femoral head necrosis were allocated to a program of either core decompression or core decompression and implantation of ATC．[Results]At review all patients had a minimum follow-up of 24-months(24 to 68 months)．In the control group，no significant improvement in Harris hip score(HHS)was found，and 13 of the 22 hips had deteriorated to stageⅢ．In the treatment group．the mean HHS was improved from 62．8 to 81．6．Collapse was seen in 1 hip，and this collapse Was progressive in 3 hips．[Conclusion]It，s thus evident that the technique is attractive as a salvage procedure．which shows encouraging Success rates and early clinical results.

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Mechanical mechanism and relative problems of lumbar traction

Yulai LU ; Xishan ZHANG ; Qiwei SUN ; Ruichen WANG ; Hu LI

Orthopedic Journal of China.2008;16(5):344-348.

[Objective] To discuss the mechanism and relative problems of lumbar traction. [Method] Relevant articles and retrospect clinical data in the author's hospital were reviewed. Review relevant articles and retrospect clinical data of our hospital. [ Result ] Traction force : 40 kg + 15% ～ 20% of body weight, fineness rate reached 83.5% in 1606 patients being treated. According to course of disease, fineness rate was 90. 1% in the group of less than 3 years, 68.2% in the group of more than 3 years. [ Conclusion] Lumbosacral nerve root leave the peak of the protruding nucleus and establish a new harmonious "root-disc" relationship after traction. The pressure and tension to the nerve root reduces or disappears, meanwhile, the pain of low back and leg is alleviated or eradicated. Appropriate traction weight and correct traction body posture are key factors of good therapeutic effect.

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Measurement of resection heights in primary total knee arthroplasty

Shui SUN ; Malzer UDO ; Schuler PETER

Orthopedic Journal of China.2008;16(2):105-110.

[Objective]A modified femoral resection method Was introduced for total knee replacement,by which the resection ofthe posterior femoral condyle was increased.This study is to verify the accuracy of resection.[Methods]In 81 knees undergoingtotal knee replacement,the thickness of vKrious cuts were measured and compared with the height of the implants.The mean val-ues of these measurements were calculated,and eompqrisons were made according to the mechanical axis using Student's t-test.[Results]On average,the thickness of bone resection in both the flexion and the extension gap was smaller than the thickness otthe prosthesis.In the varus group a minimum lateral over-resection in the flexion gap Was found,and in valgus group a minimummedial over-resection in the flexion gap was found.[Conclusion]The early clinical results with this modified resection method aregood.

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Open reduction and internal fixation of displaced radial head fractures

Hongchuan WANG ; Shilian KAN ; Guigen PANG ; Xiantie ZENG ; Xin CHEN

Orthopedic Journal of China.2008;16(6):426-429.

[Objective]To explore the operative techniques of open reduction and internal fixation of displaced radial head fractures in adults. [Methods]Twenty-six cases of radial head fractures had been treated with open reduction and internal fixation. According to Mason classification, 16 fractures were type II, 6 were type III and 4 were type IV. Operative exposures of radial head were performed through the Kocher approach in 22 cases, and through a midline posterior skin incision in 4 cases in order to repair concomitant fracture of proximal ulna. After being reduced, fractures of radial head were fixed with mini-fragment plate and screw which were placed in "safe zone" not to impinge on the proximal radioulnar joint with forearm rotation.[Results]At a mean follow-up of 32 months, all fractures had united. There was no patient who suffered from wound infection or injury to posterior interosseous nerve. According to the Broberg and Morrey functional elbow index, the outcome were excellent in 17 patients, good in 9.[Conclusion]Open reduction and internal fixation of displaced radial head fractures may get satisfying outcomes, with successful performing of the requirements below: (1) sustaining lateral ulnar collateral ligament; (2) avoiding injury to posterior inerosseous nerve; (3) getting anatomic reduction; (4) stably fixing fractures without impinging on joint.

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