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The primary study of optimization parameters of ultrasonic microbubbles delivery hAng -1 gene into 2 9 3 T cells in vitro

Qing ZHOU ; Qian CHEN ; Xiao WANG ; Jinling CHEN ; Ruiqiang GUO

Chinese Journal of Ultrasonography.2009;18(12):1076-1079. doi:10.3760/cma.j.issn.1004-4477.2009.12.021

Objective To certificate the effects on transfection ratio and cells viability of ultrasound (US) acoustic intensity, radiation duration, microbubbles concentration and DNA concentration in delivery human angiopioetin-1 gene (hAng-1) into 293T cells by SonoVue microbubbles and decide the optimal transfection parameters. Methods Mix 293T cells and SonoVue microbubbles linked with eGFP-C_3-hAng-1 in a different way, detect the gene transfection ratio and cells viability under the various US intensity, radiation duration, microbubbles and DNA concentrations. Results The gene expression would be increased if enhanced the intenstiy of US,radiation time,microbubbles and DNA concentrations,and the cells viability would be kept more than 90% ( P <0. 01). Whereas,if the US intensity increased over 1. 5 W/cm~2 ,the duration over 30 s and microbubbles and DNA concentrations over 20% and 15 mg/L respectively,the gene expression would not increase significantly ( P > 0. 05),whereas coupled with obviously decreased cells viability( P <0. 01). Conclusions The optimal conditions of deliver hAng-1 gene into 293T cells by SonoVue microbubbles was mixing cells and microbubbles in a cell wall-sticky way,US intensity was 1. 5 W/cm~2, duration 30 s,20% microbubbles and 15 mg/L DNA concentration.

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Investigation of right ventricular longitudinal systolic function by velocity vector imaging

Haijun HOU ; Chunling LI ; Xiaojuan ZHANG ; Guang ZHI

Chinese Journal of Ultrasonography.2009;18(12):1068-1070. doi:10.3760/cma.j.issn.1004-4477.2009.12.019

Objective To investigate the feasibility and accuracy of velocity vector imaging(VVI)to assess strain and strain rate of right ventrieule(RV)during varying myocardial functional states.Methods VVI of the longitudinal RV were recorded in 7 closed-chest anesthetized mongrel dogs from the apical 4-chamber view during 3 different inotropic states.Peak dp/dt from the RV contraction during the right cardiac catheterization,which was used as the gold standard of RV contractility,was obtained simultaneously in each inotropic state.Peak systolic velocities(V), peak systolic strain rates(SR),peak systolic strains(S)at the basal, middle and apical segments of RV free wall and septal from the longitudinal axis were quantitatively measured by using VVI software system respectively.Results The peak systolic strain and strain rate of the longitudinal RV free wall and septal were increased with the infusion of dobutamine and decreased with the infusion of metoprolol.A significant correlation was found between peak RV dp/dt and RV mean strain and mean strain rate(r=0.79, 0.75;P <0.01).Among all RV segments, the peak systolic S,SR of RV basal free wall had the most significant correlation to peak dp/dt (r=0.83,0.78;P<0.01).Conclusions Strain rate imaging drived from VVI could quantitatively and sensitively detect the longitudinal RV contractile function.Peak systolic S and SR determined at the longitudinal RV basal free wall were the strong noninvasive indices of RV contractility.

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Contrast-enhanced sonographic features of low grade and high grade dysplastic nodules in cirrhotic liver

Manxia LIN ; Huixiong XU ; Xiaoyan XIE ; Mingde Lü ; Zhi LI ; Xiaohua XIE ; Zuofeng XU ; Guangjian LIU ; Lida CHEN

Chinese Journal of Ultrasonography.2009;18(12):1057-1060. doi:10.3760/cma.j.issn.1004-4477.2009.12.015

Objective To investigate the features of low grade dysplastic nodule(LGDN)and high grade dysplastic nodule(HGDN)in cirrhotic liver on the baseline ultrasound(BUS)and contrast-enhanced ultrasound (CEUS).Methods Seventeen LGDNs and 16 HGDNs which were pathologically proved underwent baseline and CEUS examination.CEUS was performed with contrast pulse sequence(CPS)and contrast harmonic imaging (CHI)and used SonoVue as contrast agent.Results Significant differences(all P>0.05)were not shown between LGDN and HGDN for manifestations on both the BUS and CEUS.After contrast agent injected, hyperenhancement in arterial phase and hyper-or iso-enhancement in late phase,hypo-enhancement in early arterial phase and iso-enhancement through late arterial,portal and late phase,hyper-enhancement in arterial phase and hypo-enhancement in late phase,iso-enhancement in arterial,portal and late phase,iso-enhancement in arterial phase and hypo-enhancement in late phase were showed in 10(30.3%),10(30.3%),8(24.3%),4(12.1%),1(3.0%)cases of DN, respectively.Conclusions LGDN and HGDN displayed similar feature on both the BUS and CEUS.There were complicated manifestations of DN on the CEUS and the main contrast-enhanced sonographic feature of DN was,different from regenerative nodule and hepatocellular carcinoma.CEUS was helpful in diagnosing DN in cirrhotic liver.

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Evaluation of right ventricular systolic function by measurement of tricuspid annular displacement with speckle tracking imaging

Jianhua WANG ; Xin LIU

Chinese Journal of Ultrasonography.2009;18(12):1018-1021. doi:10.3760/cma.j.issn.1004-4477.2009.12.002

Objective To explore the value of peak systolic displacement of tricuspid annulus(TAD)in evaluation of right ventricular systolic function by speckle tracking imaging(STI).Methods The study population consisted of 24 healthy subjects(control group)and 16 patients with congestive heart failure(CHF).The apical four-chamber view was obtained and imput to Qlab 6.0 workstation. The TAD at the right ventricular free wall (TADla)and the midpoint of tricuspid annulus(TADmid)were measured and calculated automatically.Right ventricular ejection fraction(RVEF)was measured by real-time three-dimensional echocardiography.The correlation of displacement parameters with RVEF was analyzed.Results In all 40 subjects, the mean value of TADla was(19.3±5.9)mm with s range from 7.8 to 28.4 mm while the mean value of TADmid was(15.3±4.6)mm with a ranging from 7.2 to 22.2 mm.The parameters in CHF group were significantly lower than those of the control group(P<0.01).TADla and TADmid showed an excellent correlation with RVEF(r=0.87 and 0.91 respectively,P<0.01).The cut-off value of TADla for RVEF<45%was 17.2 mm with a sensitivity of 89%and a specificity of 93% while the cut-off value of TADmid for RVEF<45% was 13.1 mm with a sensitivity of 96%and a specificity of 1 00%.Conclusions TAD by STI showed an excellent correlation with RVEF, and it may be a new promising method for routine evaluation of right ventricular systolic function.

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The alterations of chemokines related to dendritic cells in rats with hepatic carcinoma treated by radiofrequency ablation

Zhihui FAN ; Minhua CHEN ; Weide DAI ; Wei WU ; Ying DAI

Chinese Journal of Ultrasonography.2009;18(11):983-985. doi:10.3760/cma.j.issn.1004-4477.2009.11.025

Objective To investigate the changes of chemokines related to dendritic cells in liver and spleen in rats with hepatic carcinoma treated by radiofrequency ablation (RFA),and to explore the mechanism of anti-tumor responses to RFA.Methods Forty healthy SD rats with established animal model of hepatic carcinoma were randomly divided into control group (n=10),RFA 7d group (n=16) and RFA 14d group (n=14).The rats of control group were killed without treatment.The other rats were killed in 7d and 14d after RFA treatment respectively.Spleen and liver tissue around the ablation area or around the tumor were taken out.The expressions of macrophage inflammatory protein (MIP)-1a in liver tissue and MIP-3β in spleen were analyzed by ELISA.Results The expression of MIP-1a in liver tissue was (232.92±54.5B)ng/L in control group,which enhanced to (499.38±15.14)ng/L and (495.90±9.94)ng/L in RFA 7d and 14d groups respectively.There were significant differences between control and RFA 7d group,control and RFA 14d group(P＜0.05).The expression of MIP-3βin spleen was (70.08±2.67) ng/L in control group,which enhanced to (151.57±48.48)ng/L and (154.57±18.25)ng/L in RFA 7 d and 14 d groups respectively.There were significant differences between control and RFA 7 d group,control and RFA 14 d group (P＜0.05).Conclusions The expressions of MIP-1a in liver tissue and MIP-3β in spleen increase significantly after RFA.These changes will promote recruitment and migration of dendritic cells and may contribute to the anti-tumor responses after RFA.

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Echocardiographic evaluation of persistent left superior vena cava in fetus

Weimiao YAO ; Jiale QIN ; Junmei WANG ; Yuan LI ; Lulu ZHOU ; Yue QIAN ; Hong LU ; Jing HE

Chinese Journal of Ultrasonography.2009;18(11):960-962. doi:10.3760/cma.j.issn.1004-4477.2009.11.016

Objective To evaluate the ultrasonic feature and clinical significance of persistent left superior vena cava(PLSVC)in fetal life.Methods Fetal echocardiography was performed in 3368 fetuses.Thirty-one fetuses of PLSVC were confirmed.Results The dilated coronary sinus was observed in 30 of 31 fetuses.Congenital heart defects were presented in 14 of these cases,and extracardiac anomalies were presented in 6 fetuses.Both congenital heart defects and extracardiac anomalies were observed in 4 fetuses.Conclusions PLSVC is always associated with congenital heart defects.The prognosis Of affected fetuses largely depends on whether or not the PLSVC is associated with a congenital heart defect.Prenatal diagnosis of PLSVC can help US plan perinatal counseling and ameliorate the postnatal course.

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The diagnostic value of combining real-time three-dimensional ultrasonography with three-dimensional power Doppler ultrasound for small ovarian masses

Hong XIANG ; Ling HE ; Yongquan Lü

Chinese Journal of Ultrasonography.2009;18(11):970-972. doi:10.3760/cma.j.issn.1004-4477.2009.11.019

Objective To evaluate the diagnostic value of combining real-time three-dimensional ultrasonography with three-dimensional power Doppler ultrasound for the patients with small ovarian masses.Methods One hundred and seven patients with small ovarian masses were examined with real-time three-dimensional ultrasonography and three-dimensional power Doppler ultrasound.The images and the relationship between benign and malignant small ovarian masses were analyzed.Results Benign and malignant small ovarian tumors had statistical differences in these three parameters:the envelope,inner wall and whether there being central vessels.The sensitivity and specificity of combining real-time threedimensional ultrasonography with three-dimensional power Doppler ultrasound to diagnose malignant small ovarian tumors were 92.9% and 97.3% respectively.Conclusions Combining real-time three-dimensional ultrasonography with three-dimensional color Doppler energy can be used to diagnose the small ovarian masses and provide valuable information to identify malignant ovarian tumors.A small ovarian tumor with incomplete envelope,irregular inner wall and there being central vessels indicated the possibility of being malignant.

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Assessment of fetal lung volumes using three-dimensional ultrasonography

Zhen XIAO ; Xiaoyan MA

Chinese Journal of Ultrasonography.2009;18(11):963-965. doi:10.3760/cma.j.issn.1004-4477.2009.11.017

Objective To build a nomogram of normal fetal lung volumes (left,right and total)throughout gestational age estimated by 3-dimensional ultrasonography.Methods Left and right lung volumes were assessed in 324 normal and singleton fetuses of gestational age 16-37 weeks by 3-dimensional ultrasonography using the technique of virtual organ computer-aided analysis(VOCAL) at a rotational step of 30 degrees.The relationships between left,right,and total lung volumes,and gestational age were assessed by correlation and regression analysis.Results The right,left,and total lung volumes were highly correlated with gestational age (P＜0.001),and the correlation coefficient was 0.966,0.973 and 0.990respectively.The best-fit exponential curve regression equations of left lung volume was:y=0.207exp~(0.143X);right lung volume was:y=0.301 exp~(0.14X);total lung volume was:y=0.508 exp~(0.142X).Conclusions A nomogram of fetal lung volumes estimated by 3-dimensional ultrasonography from 16 to 37weeks gestation is described,and reference values may be useful for the prenatal prediction of pulmonary hypoplasia.

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Evaluation of contrast-enhanced ultrasonography in diagnosis of focal splenic solid lesions

Jiaying CAO ; Hui ZHANG ; Wenping WANG ; Beijian HUANG ; Hong DING

Chinese Journal of Ultrasonography.2009;18(11):946-949. doi:10.3760/cma.j.issn.1004-4477.2009.11.010

Objective To investigate the value of contrast-enhanced ultrasonography(CEUS) for differential diagnosis of focal splenic solid lesions.Methods Thirty-two patients with 32 focal splenic lesions were examined with CEUS.The enhancement phases and perfusion were observed.Each group has 16 patients.Results The contrast media extincted more quickly in malignant lesions than in benign ones.The peak time and initial decreasing time of the malignant lesions were (19.7±4.5)s and (29.7±10.9)s.The opposite time of the benign lesions were (24.2±4.4)s and (50.9±22.8)s.The initial decreasing time of the malignant lesions was earlier than that of the benign ones.The enhancement pattern was similar between the two groups.More lesions can be detected after CEUS.The number of the lesions was 111 before CEUS and 142 after CEUS.Conclusions CEUS is useful in the differential diagnosis of splenic lesions.

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Correlations between quantitative parameters of contrast-enhanced ultrasonography and histopathologic stage of liver fibrosis

Na LI ; Hong DING ; Xiyuan LIN ; Peili FAN ; Chen XU ; Wenping WANG

Chinese Journal of Ultrasonography.2009;18(11):942-945. doi:10.3760/cma.j.issn.1004-4477.2009.11.008

Objective To investigate the correlations between quantitative parameters of contrast-enhanced ultrasonography(CEUS) and histopathological stage of liver fibrosis.Methods Sixty cases of hepatic fibrosis diagnosed pathologically were examined with CEUS.The dynamic images of CEUS were analyzed off-line with a quantitative software.Region of interest (ROI) were drawn separately in hepatic artery(HA),liver parenchyma(PA),main portal vein(PV) and right hepatic vein(HV).The time intensity curve(TIC) and some quantitative parameters,including arrival time,increased signal intensity(ISI),peak intensity(PI) and area under curve(AUC) were acquired.Intrahepatic transit time of contrast agents were calculated as the arrival time difference between hepatic artery(HA) and HV(HA-HVTT),between PV and HV(PV-HVTT) and between PA and HV (PA-HVTT).All the parameters were compared with histopathologic stage.Results All cases were grouped into mild fibrosis (S0 and S1,25 cases),moderate fibrosis (S2 and S3,17 cases) and severe fibrosis (S4,18 cases) according pathologic stage.HA-HVTT,PV-HVTT and PA-HVTT decreased with the development of liver fibrosis.The parameters of ISI,PI and AUC obtained from TIC of portal vein reduced with the progression of fibrosis,too(P＜0.05).Receive operating characteristic(ROC) curve analysis showed that the area under curve of HA-HVTT,PV-HVTT and PA-HVTT were 0.808,0.848,and 0.823 (S＞S1),and 0.856,0.903,and 0.806 (S＞S3),respectively.Conclusions Quantitative parameters of CEUS reflect the stage of liver fibrosis mediately.The parameters of HA-HVTT,PV-HVTT and PA-HVTT are closely correlated with the stage of liver fibrosis.

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