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The study on intratumoral heterogeneity of DNA ploidy pattern in hepatocellular carcinoma by flow cytom- etry

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To verify whether there is intratumoral heterogeneity of DNA ploidy pattern in hep- atocellular carcinoma.Methods Using paraffin-embedded specimens resected from 29 patients with untreated hepatocellular carcinoma (HCC) and having undergone partial hepatectomy,DNA ploidy was analyzed at different sites of the same HCC by means of flow cytometry.Results There was no case in which diploid and aneuploid HCCs cells coexisted in the same tumor.The DNA ploidy pattern was diploid in 13 and aneuploid in 16 cases.A- mong 16 aneuploid tumors,the DNA indices at different sites were similar in 9,but different aneuploid subclones coexisted in 7 cases.Conclusions It is suggested that aneuploid subpopulation from diploid is rare,but new ane- uploid subclones could evolve from aneuploid HCC due to increased instability of their karyotype.

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Change of calcimn metabolism in liver mitochondria and its effect on mitochondrial respiratory function fol- lowing biliary obstruction

Jing LI ; Yanbang CHI ; Xuejun FANG

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To explore the change of liver mitochondrial calcium metabolism and its effect on mitochondrial respiratory function.Methods The model of dog biliary obstruction was made by bile duct ligation. Mitochondria were obtained by differential centfifugation.Mitochondrial calcium content,calcium uptake rate and respiratory function were observed and compared.Results Liver mitochondrial calcium content was increased sig- nificantly,calcium uptake rate and respiratory function were obviously reduced after biliary obstruction.Mitochon- drial calcium content was highly negatively correlated with mitochondrial respiratory function (P

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Recovery of patients with severe acute pancreatitis after non operative treatment.

Binghuang WANG

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To observe the outcome and consequential treatment during recovery of patients with severe acute pancreatitis (SAP) after non operative treatment.Methods The data of the follow up for 1 to 12 years in 41 cases of SAP with pseudocyst of pancreas, recurrent pancreatitis, chronic abdominal pain and gallbladder stones examined with CT, ERCP, laparotomy or pancreatic tissue biopsy from 3 months to 2 years were retrospectively analyzed. Results Pseudocyst of pancreas was the main complication of SAP after non operative treatment. The absorption of necrotic tissues of pancreas and proliferation of fibrotic tissue took a slow course of 3 to 6 months. The long term excellent curative rate was 73.2% and the rate of abdominal pain or maldigestion was 24 4%. One patient died of recurrent pancreatitis (2 4%). Conclusions More attention should be paid to treating pseudocyst of pancreas, resecting the gallbladder stones in time and preventing or treating recurrent pancreatitis after the recovery from SAP.

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Protective effects of protease inhibitor on liver function after ischemia reperfusion and orthotopic liver transplantation in rats

Guichen LI ; Yongfeng LIU ; Jialin ZHANG

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To investigate the role of neutrophils and protease inhibitor in the pathogenesis of hepatic ischemia reperfusion injury. Methods The models of liver ischemia reperfusion (LIR) and orthotopic liver transplantation (OLT) were established in rats. The contents of serum ALT and LDH and liver myeloperoxidase(MPO) were determined. Meanwhile, the hepatic pathohistological changes were observed and the number of infiltrating neutrophils counted. As for the LIR model, the animals were administered with aprotinin (AP) before ischemia. Then reperfusion was achieved 45 min after ischemia. The samples were collected after reperfusion for 4 h. In OLT model, the animals were administered with AP 1 h before the transplantation and the samples were collected 4 h postoperatively. Results The contents of serum ALT and LDH and liver MPO were significantly higher in the control group than in the experimental one (P

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Management of bile duct injury in gallbladder bed

Genjun MAO ; Jingwei JI ; Zhangdong ZHEN

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To explore the causes for the bile duct injury in gallbladder bed and investigate its diagnosis and management. Methods The data of 37 cases of bile duct injury in the gallbladder bed after cholecystectomy between January 1988 and March 1998 were retrospectively analyzed. Results According to the operative records, 17 among the 37 cases had leakage from Luschka bile duct, 4 from the cholecystohepatic duct and 3 from the right lobular hepatic duct and its branches. The sources of the leakage were not identified in other 17 cases. The injured sites were sutured and drained or drained alone in 30 cases. The other 4 cases without drainage were percutaneously under the ultrasonographic guidance because of an intra abdominal bile collection. The remaining 3 cases were surgically treated again for aggravation of the illness in them. Conclusions During performance of cholecystectomy, surgeons should pay close attention to the bile duct in the gallbladder bed for its vulnerable position. Careful inspection of the gallbladder bed should be a routine procedure after the operation. Once the bile leakage was recognized in the gallbladder bed, intraoperative drainage must be performed.

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Protective effects of G CSF on lungs in rats with acute cholangitis

Xingwu YANG ; Ran FAN ; Yiwen MENG

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To investigate the protective effects of G CSF on lungs of rats with acute cholangitis and changes in blood TNF and TXA 2/PGI 2 by treating the rats with G CSF. Methods Seventy two Wistar rats were randomized into the control, infected and treated groups. We established the model of cholangitis in the rats with injection of E. coli 25922 into the bile duct. The animals in the treated group were pretreated with G CSF 500 ng/day bid) for 5 days before the E. coli injection. The levels of blood TNF and TXA 2/PGI 2 were measured at the 3rd, 6th and 12th h after the injection. Meanwhile, the morphological changes in lungs of the rats were observed. Results The morphological changes in lungs were milder and levels of blood TNF and TXA 2/PGI 2 were significantly lower in the treated group than in the infected one (P

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Therapeutic effects of sodium cholate, lactulose and anisodamine on endotoxemia in patients with obstructive jaundice

Dezheng XU ; Mingrong HU ; Xiong WANG

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

0.05) while the level was significantly higher in the former 4 groups than in the control (P

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Detection of p53 and p21~(WAF1) in cholangiocarcinoma

Xin WANG ; Jianxi ZHANG ; Jie CHEN

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To clarify the clinical significance of p21 WAF1 and the relationship between it and p53. Methods Samples of human cholangiocarcinoma (HC) tissue and paired normal bile duct tissue adjacent to the tumor from 30 patients with HC were employed in this study. In situ hybridization (ISH) was used to detect the p53 and p21 mRNA expression. Immunohistochemistry (IHC) was applied to analyze p53 gene mutation and P21 protein expression. Results p21 WAF1 And p53 gene protein expression was detected in 36.7% (11/30) and 53.3% (16/30) of the carcinoma specimens, respectively, and none of the paired tissue by IHC. p53 Positivity was related to local lymph node metastasis. Comparing with non-lymph node metastatic group, p53 positivity in metastatic group was significantly higher. p53 Positivity of cholangiocarcinoma in clinical stage III was significantly higher than that in clinical stages I and II. The survival time was significantly shorter in patients with P53 protein expression than in those without. It was found that p53 expression was not associated with p21 WAF1 expression. Conclusions P53 Positivity may be correlated with tumor development but not tumor occurrence. The fact that p53 expression was not associated with p21 WAF1 expression indicates that p53-independent activation of p21 WAF1 may exist.

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Relationship between gallbladder carcinoma and anomalous arrangement of pancreaticobiliary ductal system

Yang YANG ; Shijun KANG ; Aimin LI

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To investigate the relationship between gallbladder carcinoma (GBC) and anomalous arrangement of pancreaticobiliary ductal system (AAPB) through studying the proliferation of gallbladder epithelial cells. Methods ABC Immunohistochemical staining was employed. Results Ki 67 And proliferating cell nuclear antigen PCNA labeling indexes of the non cancerous mucosa in the patients with AAPB and GBC were significantly higher than those in patients with GBC alone (P

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Early diagnosis of primary gallbladder carcinoma

Jingsen SHI ; Gang LIU ; Yueli YU

Chinese Journal of Hepatobiliary Surgery.1998;0(06):-.

Objective To promote the level of early diagnosis and strengthen the understanding of pathogenesis, pathological staging and prognosis of primary gallbladder carcinoma (PGC). Methods The data of 679 patients with PGC treated in our hospital from 1956 to 1998 were retrospectively analyzed. Results The incidence of PGC has been increasing in recent years while the surgical management for the disease was not satisfactory. Upon diagnosis, most patients were with PGC in advanced stage. PGC was usually found among the aged woman patients. The female/male ratio was 3∶1. There was a close relationship between gallstone and PGC, for the gallstone was found in 60% of the patients with PGC. The diagnostic accordance rate before and after operations was low. Most cases of PGC were found unexpectedly during operation due to gallstone or acute cholecystitis. It was even worse that many patients with PGC missed the opportunity of diagnosis and treatment because doctors noticed the gallstone only. Pathological classification revealed that most cases of PGC were of adenocarcinoma. Development in imaging medicine might help a lot in finding early-stage cases and improving prognosis. Conclusions Strengthening the understanding of pathogenesis, pathological staging and prognosis of the disease and proper use of various examinations are the basic means of obtaining early diagnosis and improving the prognosis.

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