Chinese Journal of Experimental and Clinical Virology
1987 to Present ISSN: 1003-9279
Year of publication
Sort by
Format:
Per page:
Fever with thrombocytopenia associated with a novel bunyavirus in China.
Chinese Journal of Experimental and Clinical Virology.2011;25(2):81-84.
Strengthen the etiology study of viral acute respiratory tract infection in children.
Chinese Journal of Experimental and Clinical Virology.2011;25(1):1-1.
To enhance viral diarrhea surveillance in China.
Chinese Journal of Experimental and Clinical Virology.2012;26(1):1-1.
Construction and expression of a novel HBeAg binding protein 1 of hepatitis B virus in yeast.
Chinese Journal of Experimental and Clinical Virology.2011;25(6):489-491. doi:10.3760/cma.j.issn.1003-9279.2011.06.027
OBJECTIVETo construct the eukaryotic expression vector of HBEBP1 gene and express HBEBP1 recombinant protein in yeast.
METHODSPCR was performed to amplify the gene of HBEBP1 from the cDNA template origining from HepG2, and the gene was cloned into pGEM-T vector. After sequencing, the correct DNA fragment was cut from pGEM-T-HBEBP1 and inserted into yeast expression plasmid pGBKT7. The reconstructed plasmid pGBKT7-HBEBP1 was transformed into yeast cell AH109 and screened on the synthetic dropout nutrient medium (SD/-Trp/Kana). The yeast protein was isolated and analyzed with SDS-PAGE and Western Blot.
RESULTSThe eukaryotic expressive vector was constructed successfully. The results of Western Blot showed HBEBP1 protein was existed within yeast cells and the molecular weight of it was about 33 x 10(3).
CONCLUSIONSThe successful expression of HBEBP1 protein in yeast cells lay the foundation for studying biological function of HBEBP1.
Chinese Journal of Experimental and Clinical Virology.2011;25(5):374-376.
OBJECTIVETo discuss the epidemiological and clinical characteristics of the hospitalized children with hand foot and mouth disease (HFMD) in Yantai area.
METHODSEpidemiological and clinical data of HFMD children from 2009 to 2010 were summarized and analyzed retrospectively.
RESULTSMost of the infected (94.6%) were under 5 years old and the ratio between male and female was 1.5: 1. Oral mucosal pox or ulcer as well as hand and foot rashes were observed in all 931 patients. Fever and neurological disorders occurred in 840 (90.2%) and 121 (13.0%) patients respectively. The incidence was positively correlated with air temperature (r = 0.887, P < 0.001), with a peak in April to September (88.9%). The ratio of children from countryside, total duration of fever, serum concentration of c-reacting protein (CRP) and fasting blood glucose (FBG) were significantly higher in severe cases than in those mild ones. Multivariate analysis showed longer mean duration of fever( Odds ratio [OR], 1.491; 95% confidence interval [ CI] 1.170-1.901; P = 0.001) and hyperglycemia (OR, 1.124; 95% CI 1.016-1.245; P = 0.024) were independent risk factors of severity.
CONCLUSIONChildren younger than 5 years old are susceptible to HFMD and most cases occur in April to September. The monthly incidence is positively correlated with temperature of that month. Longer duration of fever and hyperglycemia are independent risk factors for severity. Most cases could have a favorable prognosis after timely diagnosis and proper intervention.
Strength the basic and clinical study on HBV related hepatoceluar carcinoma.
Chinese Journal of Experimental and Clinical Virology.2011;25(5):321-321.
Dog rabies control is the key point to eliminate rabies in human.
Chinese Journal of Experimental and Clinical Virology.2010;24(2):81-81.
Strengthen basic research with hepatic steatosis in HBV infection.
Chinese Journal of Experimental and Clinical Virology.2013;27(5):321-321.
Chinese Journal of Experimental and Clinical Virology.2002;16(2):146-149.
BACKGROUNDTo determine the long-term efficacy and persistence of Chinese infants after receiving only active plasma-derived hepatitis B vaccine, and to evaluate if providing booster vaccination after basic hepatitis B immunization is necessary.
METHODSInfants who were born in 1986-1988 in four demonstrative hepatitis B immunization trial areas of Hunan, Guangxi, Hebei and Shanghai after receiving only active plasma-derived hepatitis B vaccination, had been randomly followed up for 15 years. HBsAg,anti-HBs and anti-HBc in 21 680 person-times were tested using commercial SPRIA kits.
RESULTSPrevalence of HBV carriers was less than 1.66% among all children vaccinated with only active plasma-derived hepatitis B vaccine in 4 clinical trial areas. Prevalence of HBsAg did not increase with years after vaccination,90%(95% Cl:83.1%-97.2%) effectiveness of hepatitis B vaccine persisted for 15 years in preventing chronic HBV infection. Carriage, HBV infection and efficacy were not different among all age groups (P>0.05). Seroprotection rate (anti-HBs?10 mIU/ml) and quantity of anti-HBs were significantly decreased with years after vaccination. Seroprotection rates of anti-HBs were 40%-50% and 30%-42% during the 9th-10th year and the 13th-14th ear of vaccination, respectively. Titer of anti-HBs declined?by 90% after 14 years.
CONCLUSIONSThese results showed that long-term efficacy of only active plasma-derived hepatitis B vaccination, which was not affected by decline in seroprotection rate and titer of anti-HBs. For children and adults whose immune status is normal, booster doses of vaccine are not recommended.
Characterization of genome of A/Guangzhou/333/99(H9N2) virus.
Chinese Journal of Experimental and Clinical Virology.2002;16(2):142-145.
BACKGROUNDTo understand the characterization of genome of a strain of avian influenza A H9N2 virus repeatedly isolated from a child with influenza illness. Thereafter to reveal the origin of this H9N2 virus.
METHODSViruses were passed in embryonated hen eggs and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit. Afterwards RNA sequence analysis was performed by dideoxynucleotide chain termination and a cloning method. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (Version 1.03) and Editseg (Version 3.69) softwares.
RESULTSGenome of A/Guangzhou/333/99 (H9N2) virus was closely related to avian influenza A H9N2 virus, but obvious difference from that of A/Duck/Hong Kong/Y439/97(H9N2) virus, as well as its genome did not include any RNA segment derived from human influenza A virus. However, the genes encoding the HA,NA,NP and NS proteins of A/Guangzhou/333/99 virus were derived from those of G9 lineage virus, the rest genes encoding the M and three polymerase (PB2,PB1 and PA) proteins were derived from G1 lineage strain.
CONCLUSIONSA/Guangzhou/333/99 virus was a reassortant derived from reassortment betweenG9 and G1 lineages of avian influenzaA(H9N2) viruses. Therefore, the most possibility is that it is derived from avian influenza A virus directly. The results do not only demonstrate that avian influenza A (H9N2) virus could infect men, but also firstly prove that the genetic reassortment could be occurred between different genetic lineages of avian influenza A (H9N2) viruses in the nature.
Country
China
Publisher
中华医学会
ElectronicLinks
https://zhsyhlcbdxzz.yiigle.com/Editor-in-chief
hungt@public3.btas.net.cn
Abbreviation
Chinese Journal of Experimental and Clinical Virology
Vernacular Journal Title
中华实验和临床病毒学杂志
ISSN
1003-9279
EISSN
Year Approved
2007
Current Indexing Status
Currently Indexed
Start Year
1987
Description
历史沿革【现用刊名：中华实验和临床病毒学杂志;曾用刊名：实验和临床病毒学杂志;创刊时间：1987】，该刊被以下数据库收录【CA 化学文摘(美)(2009);CBST 科学技术文献速报(日)(2009);中国科学引文数据库(CSCD—2008)】，核心期刊【中文核心期刊(2008);中文核心期刊(2004);中文核心期刊(2000)】，期刊荣誉【中科双效期刊】。
Powered by IMICAMS(
备案号: 11010502037788, 京ICP备10218182号-8)
