Journal Detail Information

1

Cite

Share

Comparison of Treatment Effects between Targeting Arterial Embolization before Sacral Chordoma Excision and Temporary Balloon Occlusion of Abdominal Aorta during Sacral Chordoma Surgery

Haiwei LI ; Jing LIU

Journal of China Medical University.2014;(5):454-457.

Objective To compare the clinical efficacy of targeting arterial embolization before sacral chordoma excision and temporary balloon oc-clusion of abdominal aorta during sacral tumors surgery. Methods A total of 34 patients with sacral chordoma were recruited for this study and fol-lowed up. Totally 18 patients received bi-iliac artery,sacrococcygeal artery and other target arteryl embolization before excision(target arterial emboli-zation group),and the other 16 received temporary balloon occlusion of abdominal aorta during surgery(balloon occlusion group). The blood loss during operation,drained blood after operation,operation time and wound healing were recorded. Results The blood loss during operation,drained blood after operation and operation time in targeting arterial embolization group were less than balloon occlusion group. There was no statistical differ-ence in wound healing between the two groups. Conclusion Targeting arterial embolization can be more effective than temporary balloon occlusion of abdominal aorta in the control of blood loss.

2

Cite

Share

Genetic Polymorphisms of Glutathione S-transferase M1 and T1 and Evaluation of Oxidative Stress in Patients with Non-small Cell Lung Cancer

Hongyan ZHANG ; Xuwei WU ; Yi XIAO ; Mei CHEN ; Zhidong LI ; Yanli LI ; Kaifa TANG

Journal of China Medical University.2014;(5):432-436.

Objective To investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes(GSTM1 and GSTT1),and eval-uate the oxidative damage in patients with non-small lung cancer(N-SCLC). Methods A total of 110 patients with N-SCLC and 100 healthy indi-viduals were recruited in this case-control study. Multiplex polymerase chain reaction(PCR)analysis was used to identify the genotypes. The activi-ty of malondialdehyde(MDA),nitric oxide(NO),and the total antioxidant capacity(T-AOC)were detected by spectroscopic analysis using assay kits. Results The frequencies of the GSTM1,T1,and GSTM1/T1 null genotypes in the patient group were significantly higher than those in control group(OR1=2.071,P1=0.009;OR2=1.900,P2=0.024;OR3=3.258,P3=0.003). The activity of MDA and NO were obviously higher in the pa-tient group compared with the control group(P<0.001),and T-AOC was obviously lower in patient group than those in control group(P<0.001). The activity of MDA,and NO were higher but the T-AOC were lower in patients with GSTM1,T1 and GSTM1/T1 null genotypes than those in pa-tients with GSTM1,T1 and GSTM1/T1 present genotypes(P<0.001). Conclusion Our results suggest that oxidative damage may play a impor-tant role in patients with N-SCLC,and the N-SCLC patients with GSTM1and GSTT1deletion genotypes are more susceptible to oxidative damage.

3

Cite

Share

Effect of GR82334 on the Electric Stimulation-induced Increase of Dopamine Content in Anterior Cingulate Gyrus in Rats

Huan YIN ; Kuimin CHEN ; Jihao MA ; Minfan WU

Journal of China Medical University.2014;(5):426-428.

Objective To investigate the effects of GR82334 caudal veins injection(iv)or intrathecal injection(ith)on the increase of dopamine (DA)content in rats anterior cingulate gyrus(ACG)induced by heavy current stimulation of saphenous nerve(SN). Methods Totally 42 male Wi-star rats were randomly divided into six groups,including control group,sham stimulation group,SN stimulation group,GR82334(ith)group,NS (ith)group,GR82334(iv)group,and NS(iv)group. At the end of the study,rats of different groups were sacrificed,then the right side ACG were collected and weighted. ACG samples were then homogenized with 0.1 mol/L perchloric acid solution. After spinning at 10 000 r/min(4℃)for 20 min,20μL of the supernatant were harvest from each sample. High performance liquid chromatography electrochemical detection was used to mea-sure DA content. Results Heavy current stimulation of SN caused obvious increase of the DA content in ACG. GR82334(iv or ith)antagonized the significant increase of DA content in ACG induced by the stimulating SN. However,GR82334(ith)did not completely antagonized the increase of DA content in ACG induced by electric stimulating SN. Conclusion The results indicated that there is connection between SN and the dopami-nergic nervous system in ACG,and SN afferent nociceptive signals can activate ACG dopaminergic neurons to release DA. Peripheral and central NK-1 receptors are involved in the process of significant increase of DA content in ACG induced by SN afferent signals. However,there are other central paths of SN information input to ACG to induce obvious increases of DA content,in which other neurotransmitters and receptors may be involved.

4

Cite

Share

Effects of Vasoactive Intestinal Peptide on Transforming Growth Factor-β1 Expression in Lung Tissue of Asthmatic Mice with Airway Remodeling

Juan WANG ; Yunxiao SHANG

Journal of China Medical University.2014;(5):422-425.

Objective To investigate the effects of vasoactive intestinal peptide on transforming growth factor-β1(TGF-β1)expression in lung tis-sue of asthmatic mice with airway remodeling. Methods A total of 30 SPF grade female Balb/c mice were randomly divided into three groups:con-trol(group A),asthmatic(group B),and vasoactive intestinal peptide treatment(group C). Aerosolized ovalbumin was used to establish the asth-matic mice model. Pathological changes of bronchial and lung tissues were observed by HE,the perimeter of basement membrane(Pbm),internal wall area(WAi)and smooth muscle area(WAm)in bronchial lung tissue were analyzed using the pathological image. The expression levels of TGF-β1 mRNA and protein in lung tissue were detected by real-time PCR and Western blot. Results Pathologic changes including collagen deposition,in-filtration of inflammatory cells and smooth muscle layer thickness were found in group B. In addition,the WAi/Pbm and WAm/Pbm were significant-ly higher in group B than those of the group A(P<0.05),and lower in group C than in group B(P<0.05). The expression levels of TGF-β1mRNA and protein in group B were higher than both group A(P<0.05)and group C(P<0.05). Conclusion Vasoactive intestinal peptide may inhibit airway remodeling,which may be associated with reduced expression of TGF-β1.

5

Cite

Share

Effect of miR-24-2 on Proliferation of U-2OS Cell Line

Deyong LIANG ; Sheng CHEN ; Ye WANG ; Weineng FU

Journal of China Medical University.2014;(5):393-395.

Objective To explore the effect of miR-24-2 on the in vitro proliferation of U-2OS cells. Methods U-2OS cells were randomly allocat-ed into 4 groups:miR-24-2 mimic group,miR-24-2 inhibitor group,negative control group,and normal control group. MicroRNAs were transfected into U-2OS cells using Lipofectamine?2000. miR-24-2 expression level and the proliferation of U-2OS cells after transfection were detected by real-time quantitative RT-PCR(RT-qPCR)and MTT proliferation assays,respectively. Results RT-qPCR results showed that miR-24-2 level was sig-nificantly higher in the miR-24-2 mimic group and lower in the miR-24-2 inhibitor group than those in the controls,indicating the successive trans-fection. MTT proliferation assay results proved that the cell viability was significantly lower in the U-2OS cells transfected with miR-24-2 mimic and higher in inhibitor groups compared to the control. Conclusion MiR-24-2 inhibits growth of the U-2OS cells,which could be a potential biomarker in the treatment of osteosarcoma.

6

Cite

Share

Cloning and Expression Analysis of a Novel MYCT1 Transcript

Shuang FU ; Kailai SUN ; Weineng FU

Journal of China Medical University.2014;(5):388-392.

Objective To identify the transcriptional start site and clone a novel transcript variant of MYCT1(Myc target 1)for further study of its structure and function. Methods Transcriptional start site was confirmed using MYCT1 conserved sequence by 5′-RACE method and a novel MYCT1 isoform was cloned by splicing with 3′-RACE PCR product. Then,the cDNA or amino acid sequence between MYCT1 and its isoform was compared using bioinformatics server. Finally,the expression profile of this novel transcript in different cell lines was detected through RT-PCR. Re-sults A 1 106 bp transcript named MYCT1-TV(Myc target 1 transcript variant 1)was successfully cloned,and its transcriptional start site was confirmed which located at 140 bp upstream of the ATG start codon of MYCT1-TV. MYCT1-TV shows no obviously structural difference with MYCT1 and is widely expressed in various cell lines. Conclusion The transcriptional start site analysis and MYCT1-TV cloning provide an experi-mental basis for the further exploration and understanding of the function and the transcriptional regulation mechanism of MYCT1.

7

Cite

Share

Study on Changes of LFA-1 Level in Peripheral Neutrophils in Patients with Severe Preeclampsia

Jun WEI ; Wei WANG ; Huan TANG ; Xiaozhu WU ; Caixia LIU

Journal of China Medical University.2015;(3):263-266.

Objective To check the neutrophil count and the expression of cell surface adhesion molecule,lymphocyte function associated antigen?1(LFA?1)in peripheral neutrophils in severe preeclamptic patients and normal pregnant women in order to investigate the correlation of neutrophil activation with preeclampsia. Methods Totally 28 pregnant women in the department of gynecology and obstetrics in Shengjing Hospital from No?vember 2013 to January 2014 were included in the study,and divided into the severe preeclampsia group(n=14),and the control group(normal pregnant women,n=14). There was no statistically significant difference in age and gestational weeks between the two groups. The expression of LFA?1 in peripheral neutrophils was detected by flow cytometry in the two groups. Mean blood pressure(MBP)was measured in the severe preeclamptic group,and the correlation of MBP with expression of LFA?1 was analyzed. Results The neutrophil count was 8.40±2.23 ×109/L in the severe pre?eclamptic group,significantly higher than(6.71±1.58)×109/L in the control group,(P<0.05). The positive rate of LFA?1 in peripheral neutrophils was 63.25±38.025%in the severe preeclamptic patients,significantly higher than 8.32±38.65%in the control group(P<0.05). The expression lev?el of LFA?1 in peripheral neutrophils was significantly correlated with mean blood pressure in severe preeclamptic patients(r=0.64,P=0.013). Conclusion The neutrophil count and the expression of LFA?1 in peripheral neutrophils were significantly increased in severe preeclamptic pa?tients compared to normal pregnant women,and significantly correlated with severity of preeclampsia,suggesting that neutrophil activation participat?ed in the pathogenesis of preeclampsia.

8

Cite

Share

Effect of Endotoxin Tolerance on Cytokine

Xinxin ZHAO ; Rui MIN ; Lili ZHANG

Journal of China Medical University.2015;(3):259-262.

Objective To further determine the inflammation?associated cytokine changes during endotoxin(LPS)tolerance. Methods Kun?ming mice were divided into groups. The endotoxin tolerance mouse model was established with repeated injection of LPS. Serum levels of tumor ne?crosis factor?α(TNF?α),interleukin?10(IL?10)and IL?17 were determined by ELISA assay. Results The serum concentrations of TNF?α,IL?10 and IL?17 were 2 261.49±1 090.28 pg/mL,7 780.42±2 320.04 pg/mL,and 27.28±13.76 pg/mL in the LPS control group,which were increased significantly compared to the normal healthy control group. The serum concentrations of TNF?α,IL?10 and IL?17 were 1 174.19±450.91 pg/mL, 1 293.26±520.85 pg/mL,and 22.26±11.44 pg/mL in the endotoxin tolerance group. Levels of TNF?αand IL?17 were further decreased by dexameth?asone treatment during LPS tolerance inducing,while the IL?10 level did not change significantly. Conclusion Compared to the status of endotoxin inflammation,levels of TNF?αand IL?10 decreased significantly,while levels of IL?17 were not significantly changed in the status of endotoxin toler?ance. Dexamethasone could further suppress TNF?αand IL?17 levels during endotoxin tolerance inducing.

9

Cite

Share

Safety and Short-term Efficacy of Laparoscopic Assisted Distal Gastrectomy versus Open Distal Gastrectomy in D2 Radical Surgeries for Locally Advanced Distal Gastric Cancer:A Meta-analysis

Hongyan WANG ; Xiaodan ZHAO ; Guosheng LI ; Yuan FU ; Lirun KUANG ; Shijian CUI ; Zhengrong LIU

Journal of China Medical University.2015;(3):252-258.

Objective To conduct a Meta?analysis to compare the safety and short?term efficacy of laparoscopic assisted distal gastrectomy (LADG)and open distal gastrectomy(ODG)in D2 radical surgeries for locally advanced distal gastric cancer. Methods The literatures from Janurary,1990 to August,2014 on the evaluation of safety and short?term efficacy of LADG versus ODG in D2 radical surgeries for locally advanced distal gastric cancer were collected. The quality of the enrolled articles was evaluated and the software Revman 5.2 was adopted to analyze the cura?tive effect. Results Totally 25 articles met the inclusion criteria,including 5 044 patients with treatment of D2 radical surgeries for locally advanced distal gastric cancer. There was no significant difference in the cleaning number of lymph nodes between LADG and ODG in patients undergoing D2 radical surgeries for locally advanced distal gastric cancer. The operation time was longer for LADG than ODG,but the postoperative evaluation in?dexes such as the intraoperative blood loss and the short?term efficacy of LADG were superior to ODG. Conclusion In the treatment of locally ad?vanced distal gastric cancer,there were differences in the safety and short?term efficacy between LADG and ODG,and surgeons should give concern to these differences in clinical practice to select appropriate surgical approaches. Further research is still needed to explore the long?term efficacy.

10

Cite

Share

Effect of Rapamycin on Proliferation and Cell Cycle of Human Bladder Carcinoma Cell Line 5637

Guangyi SHAN ; Cheng FU ; Ang CHEN ; Bin HU ; Huan BI

Journal of China Medical University.2015;(3):230-233.

Objective To evaluate the effect of rapamycin on proliferation and cell cycle of human bladder cancer cell line 5637. Methods Total?ly 5637 cells were maintained in RPMI 1640 containing 10%fetal bovine serum,100 U/mL penicillin and 100μg/mL streptomycin for adherent cul?ture. Cells were grown at 37℃in a 5%CO2 incubator. The 5637 cells were treated with various concentrations of rapamycin solution(50 nmol/L, 100 nmol/L,200 nmol/L,and 400 nmol/L). The control group was daily treated with single RPMI 1640 without medication. The growth repression rate for 5637 cells was evaluated by MTT. Flow cytometry was used to investigate the effect of rapamycin of different concentrations on cell cycle of 5637 cells. Results Compared to the control group,rapamycin can inhibit the proliferation of 5637 cells in a concentration and time dependent manner. Rapamycin inhibited 5637 cells at G0/G1 thus inhibiting cell proliferation but not inducing apoptosis. Conclusion Rapamycin inhibited growth of 5637 bladder carcinoma cells and arrested cell cycle at G0/G1,indicating that mammalian target of rapamycin may play an important role in proliferation of 5637 cells and act as a new tumor therapeutic target in patients with bladder cancer.

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share