Journal Detail Information

1

Cite

Share

Clinical Application of Direct Stenting Technique in Emergent PCI for Patients with Acute Myocardial Infarction

Yong WANG ; Qi WANG ; Jing LIU ; Donghui ZHOU ; Yuanzhe JIN

Journal of China Medical University.2015;(5):468-471.

Objective To evaluate the efficacy and safety of direct stenting technique in emergent percutaneous coronary intervention(PCI)for pa?tients with acute myocardial infarction(AMI). Methods Totally 460 consecutive patients with acute myocardial infarction who underwent emergent PCI during August 2010 to July 2013 were involved,among whom 346 patients were assigned to the control group(traditional balloon dilation and stenting),114 patients were assigned to the experiment group(direct stenting). The safety and efficacy of direct stenting technique was observed. Results In the control group,7 cases had no reflow during operation,2 cases had reflow after the pre?expansion and 5 cases after stent implanta?tion,no similar cases in the experiment group(P=0.125 9). The follow?ups showed the control group had 2 cases of major adverse cardiac events including 1 case of acute stent thrombosis who required urgent revascularization,and 1 case of cardiac death at four days after operation;the experi?ment group had 1 case of stent thrombosis,there was no statistically significant difference(P=0.730 7). Conclusion Direct stenting technique may be performed selectively for certain coronary diseases when performing emergent PCI for AMI.

2

Cite

Share

The Effect of Hypoxia on Anoikis in High-metastatic Human Lung Cancer Cell Line 95D

Yan WANG ; Xin CHEN ; Xuling GAO

Journal of China Medical University.2015;(5):456-460.

Objective To investigate the effect of hypoxia micro?environment on anoikis in human high?metastatic cell line 95D of giant cell carci?noma of lung. Methods Suspension technology was used to culture 95D to establish the model of anoikis?resistant 95D cells. Hypoxic culture was conducted in the experimental group,and regular culture was conducted in the control group. The effect of hypoxia on proliferation of anoikis?resis?tant 95D was investigated by MTT and the apoptosis in the two groups were detected by flow cytometer. The invasive ability of the cells was assessed by Transwell test. The effect of hypoxia on the expression of HIF?1α,VEGF and MMP?2 in anoikis?resistant 95D was detected by Western blot. Re?sults The growth rate of the anoikis?resistant 95D cells treated with hypoxia was 52.9%,and the apoptosis rate of these cells was higher than that in the non?hypoxic group(40.4%vs 21.7%,P<0.05). The treatment of hypoxia down?regulated the invasive ability,the number of migration cells un?der hypoxia was higher than that in the control group,with statistical significance(40.1±6.7 vs 12.5±7.9,P<0.05). The up?regulation of HIF?1α, the down?regulation of VEGF and MMP?2 were observed in the group of hypoxia. Conclusion During anoikis of human high?metastatic lung can?cer cell line 95D,hypoxia inhibited the survival ability and the metastasis ability of anoikis?resistant cells,which,however,might be the early mani?festation of hypoxia.

3

Cite

Share

Clinical Features and Characteristics of Coronary Artery Lesion between Hui and Han Nationality Young Patient with Acute Myocardial Infarction

Ning YAN ; Hua ZHANG ; Xiaorui ZHAO ; Hui HUANG ; Guangzhi CONG ; Dapeng CHEN ; Yong SHA ; Shaobin JIA

Journal of China Medical University.2015;(5):452-455.

Objective To investigate the clinical features and characteristics of coronary artery lesion between Hui and Han nationality young pa?tient with acute myocardial infarction(AMI)who were referred to the affiliated hospital of Ningxia Medical University. Methods A total of 189 con?secutive AMI young patients(age≤44 years)who underwent coronary angiography were retrospectively retrieved from the database.Those patients with AMI were divided into Hui group(46 cases)and Han group(143 cases). The clinical features and results of coronary angiogram were com?pared between the two group. Results Compared with Han group,Hui group are more younger than Han group,high prevalence rate of diabetes, lower smoking history and lower drinking history(P<0.05). Coronary angiography showed the incidence of three?vessel lesions was significant low?er in Han group than in Hui group(P<0.05). Both group showed single vessel was the most common lesion. Conclusion Hui nationality patients with acute myocardial infraction are more younger and are are more prone to suffering from diabetes history、lower smoking history and lower drinking history than Han nationality patients. The coronary artery lesions of Hui nationality patients with acute acute myocardial infraction are more three?branch lesions than Han nationality patients.

4

Cite

Share

Establishment and Evaluation of Diagnostic Scoring System for Coronary Artery Disease in Patients with Diabetic Foot

Liwei MENG ; Caizhe YANG ; Xiaohong GUAN ; Shibai WU ; Luning WANG ; Min WANG

Journal of China Medical University.2015;(5):448-451.

Objective To establish a diagnostic scoring system for coronary artery disease(CAD)in patients with diabetic foot(DF)and evaluate its application efficiency. Methods The clinical data of 445 DF inpatients were analyzed retrospectively in this case?control study. These patients were divided into the CAD group(n=372,DF with CAD)and the control group(n=73,DF without CAD)according to the presence or absence of CAD. Risk factors were screened from related clinical factors examined through multiple logistic regression analysis for CAD in patients with DF and were assigned according to odds ratio(OR)to establish the scoring system for diagnosis of CAD in patients with DF. Application efficiency of the di?agnostic scoring system was tested by calculating area under the receiver operating characteristic(ROC)curve. Results The multiple logistic re?gression analysis showed that risk factors for CAD in patients with DF were age,male sex,the duration of diabetes≥10 years,the ankle?brachial in?dex(ABI)≤0.9,body mass index(BMI)≥25 kg/m2 and chronic renal insufficiency. According to ORassigned age(<50 years=0,50?64 years=1,≥65 years=2),sex(female=0,male=2),the duration of diabetes(<10 years=0,≥10 years=3),ABI(>0.9=0,≤0.9=2),BMI(<25 kg/m2=0,≥25 kg/m2=3)and chronic renal insufficiency(absent=0,present=3)scores. Area under the ROC curve of the diagnostic score scheme was 0.758(0.682?0.835),the standard error was 0.039,and the point of the diagnosis of CAD was 7. Conclusion The scoring system established in the study is efficacious,simple and practical,which provides an important reference for CAD in patients with DF.

5

Cite

Share

Expression and Significance of Epithelial-to-mesenchymal Transition in the Progression of Gastric Carcinoma and Lymph Node Metastasis

Miaomiao CUI ; Yuejia SONG ; Yunhe GU ; Jiping QI

Journal of China Medical University.2015;(5):438-442.

Objective To investigate the association between expression of the epithelial?to?mesenchymal transition(EMT)biomarkers and the malignant progression of gastric cancer in primary tumors and metastases and their possible correlation with progression of gastric cancer(GC). Methods The EMT biomarkers including E?cadherin,β?catenin,N?cadherin,Snail and TGF?β1 were detected by immunohistochemical method for 145 cases of gastric cancer(GC),25 cases of abnormal hyperplasia,13 cases of intestinal metaplasia,42 cases of lymph node metastasis and 40 cases of normal gastric mucosa tissues. Results Positive rates of TGF?β1,Snail,E?cadherin,β?catenin and N?cadherin were 73.5%,65.5%, 14.5%,53.1%and 35.9%,respectively,in gastric cancer tissues and 100%,100%,0%,27.5%and 2.5%,respectively,in normal gastric tissues, with a significant difference between the two groups(P<0.05). The decreased expression of E?cadherin andβ?catenin and the increased expression of TGF?β1 were related to the depth of invasion of gastric cancer(P<0.05). The expression of E?cadherin was correlated positively with the expres?sion ofβ?catenin,but negatively with the expression of TGF?β1. Whereas,the expression of N?cadherin was correlated positively with the expression of TGF?β1(P<0.05). The expression of E?cadherin andβ?catenin in lymph node metastasis was significantly higher than that in gastric cancer tis?sues,while the expression of TGF?β1 was lower than in gastric cancer tissues(P<0.05). Conclusion The increased expression of TGF?β1 and Snail and the decreased expression of E?cadherin,β?catenin,and N?cadherin are involved in the processes of invasion and metastasis of GC. The transformation of E?cadherin to N?cadherin and the expression of TGF?β1 may play an important role in the development of GC. In lymph node me?tastasis,the phenomenon of mesenchymal?to?epithelial transition(MET)occurs.

6

Cite

Share

Analysis of Bacterial Time Distribution of ICU-acquired Infections

Haiyuan WANG ; Tao HONG ; Xingmao WU ; Bin ZANG

Journal of China Medical University.2015;(5):434-437.

Objective To retrospectively analyze bacterial time distribution of ICU?acquired infections in Shengjing Hospital of China Medical Uni?versity,so as to provide reference for the early antibiotic use for ICU?acquired infections. Methods A total of 1 330 cases in ICU from Jan. 2012 to Mar. 2013 were collected,the bacterial culture was positive in 254 cases. A total of 1 110 strains were collected from all the patients. Excluding 288 strains which were detected within 48 hours of patients′admission in ICU and 222 strains which were repeatedly detected in the same patients,600 strains were finally enrolled in the statistical analysis. Results The rate of ICU?acquired infections was 19.1%. Postoperative infections accounted for 74.3%,most of which occurred after neurosurgeries,and abdominal,orthopedic operations. Pulmonary infection ranked the first in ICU?acquired infections,accounting for 40.3%,followed by blood stream infection(25.3%),postoperative drainage infection(14.2%)and urinary tract infection (7.3%). The rate of pathogenic bacteria detection was the highest in the first week of patients′admission in ICU,and was getting lower as time went by. Strains detected in ICU mainly were Bauman Acinetobacter,Pseudomonas aeruginosa,Klebsiella pneumonia and Enterococcus faecium,most of strains resulting in infections were gram negative bacilli throughout the time. In addition,the infection rate of fungi was increased at 2 weeks of pa?tients′admission in ICU. Conclusion The treatment of ICU?acquired infections should be targeted at gram negative bacilli. The detection rate of op?portunistic pathogens gradually increased with prolonged stay in ICU,most of which are non?fermentative bacteria. Fungi infections are most likely to occur at 2 weeks of patients′admission in ICU.

7

Cite

Share

Clinical Analysis of Risk Factors for Intraocular Pressure Elevation after Vitrectomy

Jingnan HAN ; Chao WAN ; Ning ZHAO ; Ningning LIU ; Limin LIU ; Na CAI ; Lei CHEN

Journal of China Medical University.2015;(5):429-433.

Objective To research the incidence and etiological factors of intraocular pressure(IOP)elevation in patients in early and late postop?erative stages after vitrectomy. Methods The clinical data of 235 cases(306 eyes)who underwent vitrectomy in our hospital were respectively ana?lyzed. IOP was measured before operation,in the early postoperative stage(within 2 weeks)and in the late postoperative stage(6 months after opera?tion or longer)by non?contact tonometer. Risk factors related with ocular hypertension happened in the early and late postoperative stages were statis?tically analyzed. Results The mean average IOP before operation was 15.3 ± 4.1 mmHg for the 306 eyes. Within the follow?up period of 6?20 months(mean,14.3 months),ocular hypertension occurred in 42 eyes[incidence,13.7%;mean,31.9 ± 6.0 mmHg]in the early postoperative stage and in 12 eyes[incidence,3.9%;mean,32.1 ± 5.7 mmHg]in the late postoperative stage. Logistic regression analysis revealed that lensecto?my,scleral buckling,cyclophotocoagulation,inert gas filling,silicone oil tamponade and diabetic retinopathy were the independent risk factors of oc?ular hypertension in early postoperative stage after vitrectomy,while lensectomy,scleral buckling,pan retinal photocoagulation,silicone oil tampon?ade,diabetic retinopathy and carotid artery stenosis were the independent risk factors of ocular hypertension in late postoperative stage after vitrecto?my. In addition,cyclophotocoagulation was a protective factor of IOP in the late postoperative stage. Conclusion The risk factors of ocular hyperten?sion after vitrectomy include the intraocular gapfiller,the mode of combined operation,disease type and carotid artery stenosis. IOP elevation that happens in the late postoperative stage is more harmful. Ocular hypertension in the late postoperative stage is not associated with that in the early stage.

8

Cite

Share

The Evaluation of Anti-tumor Effect of CTL Induced by Oxidized Mannan-modified Tumor Cells Vaccine

Jia SU ; Yutong WANG ; Xinhui QI ; Yan LI ; Di WU ; Yunpeng LIU ; Youhong JIANG

Journal of China Medical University.2015;(5):420-424.

Objective To study to take the oxidized Mannan?modified 786?0 in renal clear cell carcinom as tumor cells antigen to sensitize Dendrit?ic cells(DC)and to observe the its killing effect on renal clear cell carcinoma of CTLs induced. Methods Getting the peripheral blood mononucle?ar cells from the volunteers,and then to be stimulated to turn to be maturation by GM?CSF and IL?4 in vitro. Taking the clear renal carcinoma cell as the tumor antigen,and then making it to be modified by oxidized Mannan to acquire the tumor cell vaccine.Experimental groups include:blank group:DC?PBS group,control group:control?DC?786?0,experimental group:DC?Ox?Mannose?786?0 group. Taking the flow cytometry to detect the changes of DC phenotype,then taking the ELISA to detect the sencretion levels of supernatant of IL?12 of DC,then taking the CCK to detecte the cytotoxicity of lymphocytes(CTL)induced by DC of these experiment groups. Results Results by flow cytometry:the mature phenotype of DCs sensitized by Ox?Mannose?786?0 group included CD80,CD83,CD86 and HLA?DR expressed significantly higher than the other groups. As well as the secretion levels of IL?12. Meanwhile the cytotoxicity activity of lymphocytes(CTLs)induced by DCs which are sensitized by Ox?Mannose?786?0 increased more significantly than the other groups. Conclusion Glycosylated Antigens can be more effective in sensitizing antigen?presenting cells DC,and stimulating them to be maturation,while the killing effect to tumor cells also have noticeably improved.

9

Cite

Share

Study on the Effect of Anti Osteopontin Antibody in Treating Rats’Collagen Induced Rheumatoid Arthritis

Ying SONG ; Wei GAO ; Yi LIU

Journal of China Medical University.2015;(5):405-410.

Objective To investigate the anti?OPN antibody′s protective effect towards the synovial membrane of rats with rheumatoid arthritis,in?cluding the mechanism of such protective action. Methods From a total of 40 SD rats,6 were randomly selected as the normal control group while the remaining 34 were used to establish the collagen induced arthritis model. A total of 24 rats with arthritis index higher than 2 were chosen(exclud?ing the rest 10 rats),and randomly divided into model group and groups being treated with low,medium and high dose of anti?OPN anti?body,re?spectively. Weight,arthritis index and the volume of the left hind leg toe of the rats were recorded. Then on day 35,the rats were killed and their joints were taken out to make HE colored sections for observation of the pathological changes. The synovial membrane of each rat was taken out and WB method was used to test the protein expression of OPN and TNF?αin such membrane. ELISA method was used to test the change of the content of OPN and TNF?αin each rat′s blood. Results The rats in treatment group gained weight steadily from day 16 and their swollen joints were gradu?ally relieved upon medication. Comparing with the control group,the differences in weight,toe volume and arthritis index of the rats in model group were statistically significant(P<0.05). Comparing with the model group,the differences in weight,toe volume and arthritis index of the rats in the treatment groups were also statistically significant(P<0.05). Pathologic change of synovial membrane:for rats in treatment groups,synovial cell hy?perplasia was visibly reduced with a little inflammatory cell infiltration and smooth articular cartilage surfaces with no visible damage. Expression of OPN and TNF?αin synovial membrane and blood serum of rats:Judging from the OPN measured in synovial membrane and blood serum,we could see that the expression of OPN in rats of the model group increased obviously if compared with the control group. Comparing with the model group, the expression of OPN in the treatment groups was reduced(P<0.05). Judging from the TNF?αmeasured in synovial membrane and blood serum, we could see that the expression of TNF?αin rats of the model group increased obviously if compared with the control group,thus such difference was statistically significant between different groups(P<0.05). Conclusion The anti?OPN anti?body could significantly reduce the articular symp?toms of the rats with collagen induced arthritis,restrain the synovial cell hyperplasia and alleviate the inflammatory reaction. In the process of protect?ing the joints of the rats with collagen induced arthritis,anti?OPN anti?body restrained only the expression of OPN in the synovial membrane and blood serum,leaving the expression of TNF?αunaffected.

10

Cite

Share

Effect of Crownless Roots on Surrounding Alveolar Bone Tissues in SD Rats with Osteoporosis

Jing LI ; Hongjun AI ; Yansong HONG

Journal of China Medical University.2015;(5):400-404.

Objective To study the adverse effects on periodontal tissues caused by crownless roots in elderly people with osteoporosis. Methods Totally 60 12?week SD rats(30 male and 30 female)were randomly divided into the control group(10 male and 10 female sham?operated rats)and the osteoporosis group(20 male and 20 female castrated rats). After 12 weeks,the bone mineral density of right femora was measured by dual energy X?ray for all rats to confirm osteoporosis. The right molar crown was pinched off with haemostatic forceps and the molar root was retained. The 10 con?trol rats and the 20 experimental rats were sacrificed respectively at postoperative 2 and 4 weeks. The periodontal tissues of right molar were sampled and the morphology of the parodontium and the alveolar bone was observed by haematoxylin and eosin(HE)staining and the expression of tumour necrosis factor?alpha(TNF?α)and inducible nitric oxide synthase?2(NOS2)was detected by immunohistochemical staining and real time fluores?cent quantitative PCR(qRT?PCR). Results The HE slices indicated that the periodontal tissues in the osteoporosis group were significantly im?paired compared to the control group as the structure of parodontium loosened. Bone impairment aggravated over time. TNF?αstaining showed as a few claybank spots on the parodontium in the control group,while the staining colour was darkened in the osteoporosis group. The expression of TNF?αmRNA was significantly higher in the osteoporosis group than in the control group(P<0.05). The result of NOS2 was contrary to that of TNF?α. The differences in TNF?αand NOS2 were not statistically significant at 2 and 4 weeks(P>0.05). Conclusion The crownless roots in rats with os?teoporosis cause adverse effects of periodontal tissues as the roots accelerate absorption of the surrounding alveolar tissues. It is indicated that crown?less roots in elderly people with osteoporosis should be removed as soon as possible.

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share