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The expression of CXCR7 in osteosarcoma tissues and cells

Journal of Chinese Physician.2010;12(8):1048-1051. doi:10.3760/cma.j.issn.1008-1372.2010.08.013

Objective To study the differences of CXCR7 expression between in osteosarcoma tissues and normal peri-osteosarcoma tissues, and observe the effect on cell proliferation and inflammatory cytokine PGE2 secretion in MG63 cells when it had been transfected with pEGFP-Adv-CXCR7 vector. Methods CXCR7 expression in osteosarcoma tissues, cells and normal peri-osteosarcoma tissues detected by RT-qPCR. The CXCR7 mRNA expression, cell proliferation and PGE2 secretion of MG63 cells after transfected with pEGFP-Adv-CXCR7 expression vector were evaluated by RT-qPCR, MTT and ELISA, respectively. Results The expression rate and quality of CXCR7 mRNA in osteosarcoma tissue were 93.80% and (2. 221 ±0. 112), which significantly higher than those of normal peri-osteosarcoma tissues 45.71% and (0. 3448 ±0.098). Low expression of CXCR7 in MG63 cells was also observed. The proliferation and PGE2 secretion of MG63 cells were significantly increased at the time point of 48, 72 and 96h after pEGFPAdv-CXCR7 expression vector transfected into MG63 cells ( P < 0. 05). Conclusion The expression of CXCR7 mRNA in osteosarcoma tissues were significantly higher than normal peri-osteosarcoma tissues. CXCR7 high expression may have a negative effect on the progress of osteosarcoma.

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Clinical analysis of children with acute symptoms of vomiting and abdominal pain

Linxia WANG ; Changchong LI ; Xiaoou SHAN

Journal of Chinese Physician.2010;12(8):1052-1055. doi:10.3760/cma.j.issn.1008-1372.2010.08.015

Objective To provide the practical basis for clinical diagnosis and treatment of patients who were admitted by green channel because of vomiting and abdominal pain. Method 268 cases with vomiting andabdominal pain ( from January 2007 to December 2008 ) who were admitted into the emergency observation unit through green channel were retrospectively analyzed, and various risk factors were considered with logistic regression model. Result Among 268 cases, 34 kinds of different diseases were found,while gastrointestinal diseases were the greatest proportion [60. 1% (161/268)] and gastroenteritis was most common reason 77.6%( 125/161 ). Between different age groups ( ≤ 3-year-old group and>3-yearold group), the overall incidence of various diseases and the profile of disease was different (P<0.05).The incidence of diseases in the digestive tract, infectious diseases and surgical between two groups was statistically different (P<0.05). Between inpatient admission and non-inpatient admission patients, single logistic regression showed that there was significantly different between the nine events (age, fever, shock,duration of symptoms, blood routine test( BRT), liver function, blood Na+, blood / urine amylase, electrocardiogram) ( P <0.05). In the regression model, all showed significant difference except BRT. Conclusion Most of patients with vomiting and abdominal pain were digestive system diseases. Patients ≤ 3years old had a higher incidence of infectious and surgical diseases. The patients who accompanied by fever,shock and laboratory tests such asliver function, blood Na +, blood / urine amylase, electrocardiogram were abnormal would have higher hospitalize rate.

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Studies on the relationship between serum HBV DNA level and apoptosis of PBMC and Caspase-8 in patients with CHB

Jian GONG ; Keqing WAN ; Huanyu GONG ; Zhenguo LIU ; Haoye ZHANG

Journal of Chinese Physician.2010;12(8):1037-1040. doi:10.3760/cma.j.issn.1008-1372.2010.08.009

Objective To study the relationship between serum HBV DNA level and apoptosis of peripheral blood mononuclear cells (PBMC) , and the relationship between serum HBV DNA level and the activity of caspase-8 in the patients with chronic hepatitis B (CHB). Method 30 CHB patients were selected as experimental group, and it was divided into three subgroups according to the serum HBV DNA level, subgroup A (high serum HBVDNA), subgroup B (medium serum HBVDNA), and subgroup C (low serum HBVDNA). 10 healthy adults were random selected as control group. PBMC were isolated from two groups by separating medium of lymphocytic cell and culturing it with phytohemagglutinin (PHA) in vitro for 72 hours. The PBMC was stained with PI and the apoptosis was assayed with flow cytometry. At the same time, the aetivity of caspase-8 of PBMC was assayed by color matching. Results The apoptosis rate of PBMC of experimental group ( 26. 88 ± 7.37 ) % were higher than that of the control group ( 14. 95 ±2. 53)% ( P <0. 01 ). In the experimental group, the apoptosis rate of PBMC of subgroups A, B and C showed decreasing order (34. 75 ± 4. 59)%, (25.63 ± 3.55 )%, ( 18. 91 ± 3. 81 )%. The activity of caspase-8 of experimental group 2. 99 ±0. 82 were higher than that of the control group 1.43 ±0. 91 ( P <0. 01 ). The activity of caspase-8 of subgroup A, B and C showed the same decreasing order: 3. 87 ±0. 35,2. 95 ± 0. 36, 1.95 ± 0. 29. There was a positive correlation between the apoptosis level of PBMC and the activity of caspase-8 in experimen tal group ( r = 0. 610, P < 0. 01 ). Conclusion AICD of PBMC was found in patients with CHB. The activity of caspase-8 increased in that process, and it may participate in the transduction of apoptosis signal. Serum HBV DNA level was related with the apoptosis rate of PBMC and the activity of caspase-8, and it may be one of the reasons of apoptosis in PBMC.

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Curative effect of improved loop electrosurgical excision procedure for diagnosis and treatment of cervical diseases

Weijian TAN ; Yafei ZHAO

Journal of Chinese Physician.2010;12(8):1019-1021. doi:10.3760/cma.j.issn.1008-1372.2010.08.003

Objective To study the effect of using improved loop electrosurgical excision procedure for diagnosis and treatment of cervical diseases. Methods The clinical data of 70 cases of cervical intraepithelial neoplasia or carcinoma in situ from January 2006 to Decemder 2008 in our hospital was collected.The operation time, the mean volume of blooding, days of blooding, relationship between the mean blood and areas of pyramid area of pyramid was compared between study group and control group. Improved loop electrosurgical excision procedure was executed on 21 cases of study group ( CIN Ⅰ 4 cases, CIN Ⅱ 10 cases,CIN Ⅲ4 cases, CIS3 cases ) and loop electrosurgical excision procedure was used on 49 cases in control group (CIN Ⅰ7 cases,CIN Ⅱ21 cases, CIN Ⅲ 16 cases, CIS5 cases). Results Blood loss [(16.57 ±17. 47 ) ml], operative time [( 9.07 ± 3.34 ) min] and postoperative bleeding time [(9.10 ± 2.23) d] in study group were lower than that in control group [( 39.80 ± 37.43 ) ml, ( 12. 19 ± 5.34 ) min, ( 10. 49 ±2. 76)d], and the differences were statistically significant ( P < 0. 05 ). Cervical cone volume [( 14. 70 ±13. 35 ) mm3] and blood loss [(32. 83 ± 14. 32 ) ml] were positively correlated ( r = 0. 430, P < 0. 05 ). No significant difference between prognosis of two groups was found( P >0. 05 ). Conclusion There was positive relationship between the averge blood loss in surgery and areas of pyramid excision. It'd be better to stitch the ramus descendents of uterine artery at three and nine o'clock of the cervix uteri, when the patients were expected to have large pyramid areas and abundant blood flow who may bleed more than normal. which can reduce the blood loss after surgery and reduce the time of surgery.

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Observation on the effect of autologous bone marrow stem cells transplantation in treatment of early spinal cord injury

Jianxin ZHU ; Zhongmin LI ; Li LI ; Yanhui LI ; Shuangfeng CHEN ; Fengyang GENG ; Qiang FU ; Chuanjun GUO

Journal of Chinese Physician.2010;12(8):1056-1059. doi:10.3760/cma.j.issn.1008-1372.2010.08.016

Objective To investigate the recent efficacy and safety of autologous bone marrow stem cells transplantation in treatment of early spinal cord injury. Methods 51 cases of early spinal cord injury admitted to Liaocheng People Hospital from 2007.11 to 2009.8 were enrolled in this study. In transplantation group, 24 patients were treated by subarachnoid space injection with autologous bone marrow stem cell transplantation. The patients who were not transplanted in the same period of hospitalization were selected as control group. Motor and sensory function ( AISA score) was assessed at 1, 3, 6 months before and after transplantation in two groups patients. And blood routine, clotting mechanisms, biochemical items andtunor markers were determined in followed up. Results After one month of transplantation, two groups ofpatients had recovered in motor and sensory function to some degree. After three months of transplantation,there was significant different between transplantation group and control group in sensory function recovery (P < 0. 05 ). After 6 months of transplantation, there were significant different between transplant group and control group in motor and sensory function recovery (P<0.05). Blood examination results did not show markedly abnormal in followed -up patientsConclusion The safety and recent effect of autologous bone marrow stem cells transplantation in treatment of early spinal cord injury were satisfied, but the long - term effect was still unclear.

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Clinical symptoms and thyroid function between depressive patients with and without attempted suicide behavior

Shenglin WEN ; Minfeng CHENG ; Houliang WANG

Journal of Chinese Physician.2010;12(8):1060-1062. doi:10.3760/cma.j.issn.1008-1372.2010.08.017

Objective To explore the characteristics of both clinical symptoms and thyroid function between depressive patients with and without attempted suicide behavior. Methods Depressive patients were divided into two groups. The first group was composed of patients with attempted suicide behavior (n = 56) and the second group without attempted suicide behavior ( n = 85 ). Thyroid function (FT3, FT4 )were determined by radioimmunoassay method. The patients were evaluated by symptom checklist 90 (SCL90). Results The score of depressive factor in the group with attempted suicide [(2. 90 ± 1. 01 ) score]was higher than that of the group without attempted suicide [(2.51±0.77) score] ( ( t =2. 127, P =0. 036). The serum level of FT3 in the group with attempted suicide [(3.92 ±0. 52) pmol/L] was lower than that of the group without attempted suicide[(4.18 ±0.71) pmol/L] ( t =-2.219, P =0.028).Conclusions There were certain characteristics on clinical symptoms of depressive patients with attempted suicide. The low serum levels of FT3 might play an important role in the risk of suicide in depressive patients.

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Blood plasma heat shock protein 60, 70 expressions in child asthma patient and the risk factors of child asthma

Shuping ZHENG ; Xiaohui LI ; Hanbin SHEN ; Li YU

Journal of Chinese Physician.2010;12(8):1045-1047. doi:10.3760/cma.j.issn.1008-1372.2010.08.012

ObjectiveTo evaluate the expression of heat shock protein in child asthma patient and investigate the risk factors of child's asthma. MethodPatients with clinical data of pediatric asthma history from outpatients department in Wuhan fifth hospital and follow up data were selected. The analysis of interrelated element was used with conditional logistic regression. ResultThe result of logistic analysis demonstrated that asthma was related with these risk factors, including history of upper respiratory infection, changes of weather, individual history of sensitive disease, family history of sensitive disease and heat shock protein. Fierce sports, factors of diet, passive smoking, and etc. Were not related to the degree of danger of asthma in this study. ConclusionHistory of upper respiratory infection, changeable weather, individual history of sensitive disease, family history of sensitive disease and heat shock protein were related with the degree of asthma. It should be helpful to treat asthma with continued propagation and education to advocate standardized treatment.

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Clinical value of neoadjuvant chemotherapy in the treatment of local advanced cervical cancer

Yu ZHANG ; Xiaomao LI ; Yuebo YANG ; Mao LIU ; Yiran TAO ; Yu ZHOU ; Qiang ZHAO

Journal of Chinese Physician.2010;12(8):1029-1032. doi:10.3760/cma.j.issn.1008-1372.2010.08.006

Objectives To evaluate the clinical value of neoadjuvant chemotherapy (NACT) in the treatment of local advanced cervical cancer. Methods We searched the clinical trials on the treatment of local advanced cervical cancer with NACT followed by surgery versus initial surgery on English and Chinese published literatures from the main medical data resources (MEDLINE, PUBMED, ELSEVIER ScienceDirect, CNKI). The data about positive pelvic nodes, interstitial infiltration, vascular invasion, positive surgical margin, 3-year overall survival (OS), 3-year disease-free survival (DFS), 5-year OS, 5-year DFS were extracted from these papers, and a meta-analysis was applied. Result The hazard ratio (HR) of positive pelvic nodes on NACT group versus control group was 0. 54(95% CI 0. 33 ～0. 86), HR of interstitial infiltration was 0. 45(95% CI 0. 24 ～0. 86), HR of vascular invasion was 0. 25(95% CI 0. 16 ～0. 38), all differences were statistically significant. And HR of positive surgical margin was 0. 45 ( 95% CI 0. 21 ～0. 99), P = 0. 05, which indicated the difference was not statistically significant. And there were also no significant difference on the HR of 3-year OS, 3-year DFS, 5-year OS and 5-year DFS, and their RR were 1.18(95% CI 0. 84 ～ 1.66) ,1.31 (95% CI 0. 96 ～ 1.78) ,0. 89(95% CI 0. 68 ～ 1.15) ,and 0. 99(95% CI 0. 71 ～ 1.93) respectively. Conclusion For local advanced cervical cancer, NACT could reduce pathological risk factors but it did not improve the survival.

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Changes of biochemical mark and bone histomorphology in male rats with different age

Xiongwen ZHOU ; Xincun JIAN ; Yonghua LEI ; Dangling PANG

Journal of Chinese Physician.2010;12(8):1014-1018. doi:10.3760/cma.j.issn.1008-1372.2010.08.002

Objective To investigate the aged-related changes of biochemical mark and bone histomorphology in rats and to explore the effects ofαD3 on aged rats. Methods Sixty male Wistar rats with different age were divided into four groups, including group A (6-week-old male wistar rat), group B (6-month-old male wistar rat), group C (24-month-old male wistar rat) and group D (24-month-old male wistar rat +αD3). Group D was treated with αD3 three times per week for 10 weeks at a does of 0.05μg/(kgrameters. The left femur and mandible were collected for histomorphology. Results Compared with group A, ALP (alkaline phosphatase) and BGP in group B, C and D were decreased with age increased [(87.30 ±12.22)mmol/L VS (257.20 ±92.26) mmol/L, (55.23 ± 18. 18 ) mmol/L VS (257.20 ±92. 26) mmol/L,(46.17 ± 16. 6) mmol/L VS (257. 20 ±92. 26 mmol/L); (4. 37 ± 1.46) ng/L VS (9. 03 ±2. 04) ng/L,(3.80±1.00) ng/L VS (9.03 ±2.04) ng/L, (3.59 ±0.64) ng/L VS( 9.03±2.04) ng/L, P <0. 05]. TRACP5b in group C and D increased more than that in group B [( 15.69 ± 1.62 ) pg/L VS ( 7. 26 ±2.13) pg/L, ( 14.16 ± 1.88 ) pg/L VS ( 7. 26 ± 2. 13 ) pg/L, P < 0. 05]. Testosterone decreased significantly with age increased, but no significant difference was found among four groups. Testosterone was positive related with trabecular area of mandible. Bone gla protein was positive related with alkaline phosphatase and negative related with tartrate - resistant acid phosphatase 5b. Trabecular areas of femur increased in group C and D were greater than that in group B[(220310. 6 ±76360. 50) μm2 VS ( 105093.5 ±73242. 2) μm2, (293085.9 ±68230. 78) μm2 VS ( 105093.5 ±73242. 2) μm2, P <0. 05]. Compared with group B,the trabecular thickness of group C was increased by 53%, while trabecular separation was increased 123%. Trabecular area of group D was increased by 33% compared with group C. Conclusions Theresults showed that bone formation was decreased and absorption was increased with age increased. Testosterone was positive related with trabecular area of mandible. Alfacalcidol had the effects to promote the bone mass of mandible.

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Inhibitory effect of RNA interference targeting vascular epithelial growth factor-C on proliferation of human colon cancer cell line LOVO in vitro and In vivo

Xiaowen HE ; Ting LOI ; Daojin CHEN

Journal of Chinese Physician.2010;12(8):1009-1013. doi:10.3760/cma.j.issn.1008-1372.2010.08.001

Objective This study was to explore the inhibitory effect of shRNA-VEGF - C on growth of human colon cancer cell line Lovo in vitro and vivo. Methods Recombinant VEGF-C short hairpin RNA (shRNA) plasmid was constructed and transfected into Lovo cells. The expression of VEGF-C was detected at mRNA and protein levels by real-time reverse transcription-polymerase chain reaction (RT-PCR). In vivo study, xenograft tumors were established by injecting LOVO cells into nude mice, then shR-NA-VEGF-C were injected into the tumors, the tumor volume and weight and the incidences of lymph node metastasis were detected. Immunohistochemical staining was used to detect the lymphatic microvessel density of colon cancer tissues. Results After transfection of shRNA-VEGF-C, the mRNA of VEGF-C in Lovo cells were down-regulated. Four weeks after injection, the tumor volume and tumor weight in VEGF-C-shR-NA group were significantly smaller than that in empty plasmid group and NS group [(324. 9 ± 64. 8 ) mm3 vs. (553.5±90. 1)mm3 and (570. 1±85.4)mm3; (3.01 ±0.55)g vs (4.65 ±0.65)g and (4.75 ±0. 75)g]. The incidences of lymph node metastasis (30. 1% ) were significantly inhibited compared with empty plasmid group (50. 2% ) and normal saline group (53. 1% ). In shRNA-VEGF-C group, and microlymphatic density (15.5 ± 6. 90) was also decreased compared with empty plasmid group (24. 18 ±6. 45 ), and normal saline group (29. 59 ± 8. 21 ) ( all P <0. 01 ). Conclusion shRNA-VEGF-C can inhibit the growth of LOVO cells in vitro and vivo. VEGF-C may inhibit the lymph node metastasis of colon cancer by suppressing lymphangiogenesis.

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