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Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein.

Zhaoshou YANG ; Jihoo LEE ; Hye Jin AHN ; Chom Kyu CHONG ; Ronaldo F DIAS ; Ho Woo NAM

The Korean Journal of Parasitology.2016;54(2):239-241. doi:10.3347/kjp.2016.54.2.239

Chikungunya virus (CHIKV), a tropical pathogen, has re-emerged and has massive outbreaks abruptly all over the world. Containing many dominant epitopes, the envelope E2 protein of CHIKV has been explored for the vaccination or diagnosis. In the present study, the antigenicity of a recombinant expressed intrinsically disorder domain (IUD) of E2 was tested for the detection of the antibody against CHIKV through western blot method. The gene of the IUD of E2 was inserted into 2 different vectors and expressed as recombinant GST-E2 and recombinant MBP-E2 fusion protein, respectively. Two kinds of fusion proteins were tested with 30 CHIKV patient sera and 30 normal sera, respectively. Both proteins were detected by 25 patients sera (83.3%) and 1 normal serum (3.3%). This test showed a relatively high sensitivity and very high specificity of the recombinant E2 proteins to be used as diagnostic antigens against CHIKV infection.
Blotting, Western* ; Chikungunya virus ; Diagnosis ; Disease Outbreaks ; Epitopes ; Humans* ; Sensitivity and Specificity ; Vaccination

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Ornithodoros sawaii (Ixodida: Argasidae) Larvae Collected from Hydrobates monorhis on Sogugul and Gaerin Islands, Jeollanam-do (Province), Republic of Korea.

Heung Chul KIM ; Chang Yong CHOI ; Young Soo KWON ; Seok Min YUN ; Won Ja LEE ; Sung Tae CHONG ; Richard G ROBBINS ; Terry A KLEIN

The Korean Journal of Parasitology.2016;54(2):233-238. doi:10.3347/kjp.2016.54.2.233

The 65th Medical Brigade and Public Health Command District-Korea, in collaboration with the Migratory Bird Research Center, National Park Research Institute, conducted migratory bird tick surveillance at Sogugul and Gaerin Islands (small rocky bird nesting sites), Jeollanam-do (Province), Republic of Korea (ROK), on 30 July and 1 August 2009. Breeding seabirds captured by hands in their nesting burrows were banded, identified to species, and carefully examined for ticks during the nesting season. A total of 9 Ornithodoros sawaii larvae were removed from 4 adult Hydrobates monorhis (Swinhoe's storm petrel). The identification of the larvae of O. sawaii collected from migratory seabirds were molecularly confirmed using mitochondrial 16S rDNA primer sets.
Academies and Institutes ; Adult ; Argasidae ; Birds ; Breeding ; Cooperative Behavior ; DNA, Ribosomal ; Hand ; Humans ; Islands* ; Jeollanam-do* ; Larva* ; Ornithodoros* ; Public Health ; Republic of Korea* ; Seasons ; Ticks

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A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echinococcus multilocularis.

Tao YANG ; Sibo WANG ; Xuyong ZHANG ; Jie XIA ; Jun GUO ; Jixue HOU ; Hongwei ZHANG ; Xueling CHEN ; Xiangwei WU

The Korean Journal of Parasitology.2016;54(2):225-231. doi:10.3347/kjp.2016.54.2.225

Human and animal alveolar echinococcosis (AE) are important helminth infections endemic in wide areas of the Northern hemisphere. Monitoring Echinococcus multilocularis viability and spread using real-time fluorescent imaging in vivo provides a fast method to evaluate the load of parasite. Here, we generated a kind of fluorescent protoscolices in vivo imaging model and utilized this model to assess the activity against E. multilocularis protoscolices of metformin (Met). Results indicated that JC-1 tagged E. multilocularis can be reliably and confidently used to monitor protoscolices in vitro and in vivo. The availability of this transient in vivo fluorescent imaging of E. multilocularis protoscolices constitutes an important step toward the long term bio-imaging research of the AE-infected mouse models. In addition, this will be of great interest for further research on infection strategies and development of drugs and vaccines against E. multilocularis and other cestodes.
Animals ; Cestoda ; Echinococcosis ; Echinococcus multilocularis* ; Echinococcus* ; Helminths ; Humans ; Metformin ; Mice ; Models, Animal ; Parasites ; Vaccines

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Plerocercoids of Nybelinia surmenicola (Cestoda: Tentacularidae) in Squids, Todarodes pacificus, from East Sea, the Republic of Korea.

Joon Yup LEE ; Ji Woon KIM ; Gab Man PARK

The Korean Journal of Parasitology.2016;54(2):221-224. doi:10.3347/kjp.2016.54.2.221

A visceral helminth of the squid, Todarodes pacificus, is reported from the East Sea, the Republic of Korea. Total 39 squid samples were purchased from a fish market in Jumunjin-eup, Gangneung-si (City) from August 2014 to July 2015 and were examined for helminth parasites with naked eyes and under a stereomicroscope after opening the abdominal cavity with a pair of scissors. Whitish larval worms were mainly found in the stomach and abdominal cavity of the squid. They were detected in 25 (64.1%) out of 39 squids examined, and the infection density was 7 larvae per infected squid. Spatula-shaped larvae were 8.2×2.0 mm in average size, round to slightly flattened anteriorly, with round hatching posteriorly, and had characteristic 4 tentacles with numerous hooklets in the scolex. The larvae were identified as the plerocercoid stage of Nybelinia surmenicola by their morphological features. This finding represents a new host record and the first report of N. surmenicola infection in T. pacificus squids from the east coast of Korea.
Abdominal Cavity ; Decapodiformes* ; Helminths ; Korea ; Larva ; Parasites ; Republic of Korea* ; Stomach

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Prevalence of Clonorchis sinensis Infection among Residents along 5 Major Rivers in the Republic of Korea.

Young Il JEONG ; Hee Eun SHIN ; Sang Eun LEE ; Hyeng Il CHEUN ; Jung Won JU ; Jung Yeon KIM ; Mi Yeoun PARK ; Shin Hyeong CHO

The Korean Journal of Parasitology.2016;54(2):215-219. doi:10.3347/kjp.2016.54.2.215

Clonorchis sinensis is currently the most important parasite affecting public health problems in the Republic of Korea. We investigated the prevalence of C. sinensis infection among residents living along 5 major rivers in Korea. A total of 42,562 individual stool samples were collected from 37 localities and examined using the formalin-ether sedimentation technique. Helminth eggs were detected in 4,052 (9.5%) residents and 3,586 (8.4%) were infected with C. sinensis. The egg positive rate of C. sinensis in Nakdong, Seomjin, Geum, Yeongsan, and Han River was 11.7%, 9.9%, 6.5%, 3.1%, and 1.0%, respectively. The overall prevalence of clonorchiasis by sex was 11.2% in males and 6.2% in females. The age-prevalence was the highest in the 50-59 years band. It has been reconfirmed that the endemicity of clonorchiasis is higher in southern areas of Korea, especially along Nakdong and Seomjin Rivers. A combination of continuous control programs with health education initiatives is urgently required in these highly endemic areas of clonorchiasis in Korea.
Clonorchiasis ; Clonorchis sinensis* ; Eggs ; Female ; Geum ; Health Education ; Helminths ; Humans ; Korea ; Male ; Ovum ; Parasites ; Prevalence* ; Public Health ; Republic of Korea* ; Rivers*

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Three Nematode Species Recovered from Terrestrial Snakes in Republic of Korea.

Seongjun CHOE ; Junsik LIM ; Hyun KIM ; Youngjun KIM ; Heejong KIM ; Dongmin LEE ; Hansol PARK ; Hyeong Kyu JEON ; Keeseon S EOM

The Korean Journal of Parasitology.2016;54(2):205-213. doi:10.3347/kjp.2016.54.2.205

The majority of parasitological studies of terrestrial snakes in Korea have focused on zoonotic parasites. However, in the present study, we describe 3 unrecorded nematode species recovered from 5 species of snakes (n=6) in Korea. The examined snakes, all confiscated from illegal hunters, were donated by the Chungnam Wild Animal Rescue Center and Korean Broadcasting System in July 2014 and February 2015. Light and scanning electron microscopies on the shapes of spicules that are either bent or straight (kalicephalids) and the presence of the intestinal cecum (ophidascarids) figured out 3 nematodes; Kalicephalus brachycephalus Maplestone, 1931, Kalicephalus sinensis Hsü, 1934, and Ophidascaris excavata Hsü and Hoeppli, 1934. These 3 species of nematode faunas are recorded for the first time in Korea.
Animals ; Animals, Wild ; Cecum ; Chungcheongnam-do ; Korea ; Microscopy, Electron, Scanning ; Parasites ; Republic of Korea* ; Snakes*

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High Toxoplasma gondii Seropositivity among Brain Tumor Patients in Korea.

Bong Kwang JUNG ; Hyemi SONG ; Min Jae KIM ; Jaeeun CHO ; Eun Hee SHIN ; Jong Yil CHAI

The Korean Journal of Parasitology.2016;54(2):201-204. doi:10.3347/kjp.2016.54.2.201

Toxoplasma gondii is an intracellular protozoan that can modulate the environment of the infected host. An unfavorable environment modulated by T. gondii in the brain includes tumor microenvironment. Literature has suggested that T. gondii infection is associated with development of brain tumors. However, in Korea, epidemiological data regarding this correlation have been scarce. In this study, in order to investigate the relationship between T. gondii infection and brain tumor development, we investigated the seroprevalence of T. gondii among 93 confirmed brain tumor patients (various histological types, including meningioma and astrocytoma) in Korea using ELISA. The results revealed that T. gondii seropositivity among brain tumor patients (18.3%) was significantly (P<0.05) higher compared with that of healthy controls (8.6%). The seropositivity of brain tumor patients showed a significant age-tendency, i.e., higher in younger age group, compared with age-matched healthy controls (P<0.05). In conclusion, this study supports the close relationship between T. gondii infection and incidence of brain tumors.
Brain Neoplasms* ; Brain* ; Enzyme-Linked Immunosorbent Assay ; Humans ; Incidence ; Korea* ; Meningioma ; Seroepidemiologic Studies ; Toxoplasma* ; Tumor Microenvironment

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Identification of Cryptosporidium from Dairy Cattle in Pahang, Malaysia.

Nur Hazirah HISAMUDDIN ; Najat HASHIM ; Sharmeen Nellisa SOFFIAN ; Mohd Hishammfariz Mohd AMIN ; Ridhwan Abdul WAHAB ; Mardhiah MOHAMMAD ; Muhammad Lokman Md ISA ; Afzan Mat YUSOF

The Korean Journal of Parasitology.2016;54(2):197-200. doi:10.3347/kjp.2016.54.2.197

Cryptosporidium, a protozoan parasite, can cause cryptosporidiosis which is a gastrointestinal disease that can infect humans and livestock. Cattle are the most common livestock that can be infected with this protozoan. This study was carried out to determine the prevalence of Cryptosporidium infection in cattle in Kuantan, Pahang, Malaysia and to find out the association between the occurrence of infection and 3 different ages of cattle (calves less than 1 year, yearling, and adult cattle). The samples were processed by using formol-ether concentration technique and stained by modified Ziehl Neelsen. The results showed that 15.9% (24/151) of cattle were positive for Cryptosporidium oocysts. The occurrence of Cryptosporidium in calves less than 1 year was the highest with the percentage of 20.0% (11/55) followed by yearling and adult cattle, with the percentage occurrence of 15.6 % (7/45) and 11.8% (6/51), respectively. There was no significant association between the occurrence and age of cattle and presence of diarrhea. Good management practices and proper hygiene management must be taken in order to reduce the infection. It is highly important to control the infection since infected cattle may serve as potential reservoirs of the infection to other animals and humans, especially animal handlers.
Adult ; Animals ; Cattle* ; Cryptosporidiosis ; Cryptosporidium* ; Diarrhea ; Gastrointestinal Diseases ; Humans ; Hygiene ; Livestock ; Malaysia* ; Oocysts ; Parasites ; Prevalence

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Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina.

Pamela TESTARDINI ; María Lucía Gallo VAULET ; Andrea Carolina ENTROCASSI ; Claudia MENGHI ; Martha Cora ELISEHT ; Claudia GATTA ; Mirta LOSADA ; María Sol TOUZÓN ; Ana COROMINAS ; Carlos VAY ; Silvio TATTI ; Angela FAMIGLIETTI ; Marcelo Rodriguez FERMEPIN ; Beatriz PERAZZI

The Korean Journal of Parasitology.2016;54(2):191-195. doi:10.3347/kjp.2016.54.2.191

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.
Argentina* ; Culture Media ; Diagnosis* ; Exudates and Transudates ; Female ; Genes, rRNA ; Humans ; Polymerase Chain Reaction ; Pregnancy* ; Pregnant Women ; Sensitivity and Specificity ; Sodium Chloride ; Trichomonas vaginalis* ; Trichomonas*

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Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea.

Seung Ryong KIM ; Jung Hyun KIM ; Na Yeong GU ; Yong Suk KIM ; Yeon Chul HONG ; Jae Sook RYU

The Korean Journal of Parasitology.2016;54(2):187-190. doi:10.3347/kjp.2016.54.2.187

Trichomoniasis is the most common curable sexually-transmitted infection (STI) worldwide. There are few reports on the prevalence of Trichomonas vaginalis in Korea. The purpose of this study was to examine the prevalence of trichomoniasis by PCR in Guri city, Korea. All adult women who visited Hanyang University Guri Hospital for health screening within the National Health Care Service were invited to participate in the study, and 424 women were enrolled between March and June 2011. PCR was used to detect Trichomonas vaginalis using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Fourteen women (3.3%) were found to have T. vaginalis. All were over 50, and they were significantly older on average than the 410 Trichomonas-negative women (mean ages 63.4 vs 55.3 years). It seems that T. vaginalis infection is not rare in women receiving health screening, especially among those over 50.
Adult ; Clone Cells ; Delivery of Health Care ; Female ; Humans ; Korea* ; Mass Screening* ; Polymerase Chain Reaction* ; Prevalence* ; Repetitive Sequences, Nucleic Acid ; Trichomonas vaginalis

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