Professor Chin-Thack Soh (1921–2016).
The Korean Journal of Parasitology.2016;54(6):819-819. doi:10.3347/kjp.2016.54.6.819
No abstract available.
The Korean Journal of Parasitology
2002 (v1, n1) to Present ISSN: 1671-8925
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Sequence Analysis of Mitochondrial Genome of Toxascaris leonina from a South China Tiger.
The Korean Journal of Parasitology.2016;54(6):803-807. doi:10.3347/kjp.2016.54.6.803
Toxascaris leonina is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. To analyze population genetic characteristics of T. leonina from South China tiger, its mitochondrial (mt) genome was sequenced. Its complete circular mt genome was 14,277 bp in length, including 12 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The nucleotide composition was biased toward A and T. The most common start codon and stop codon were TTG and TAG, and 4 genes ended with an incomplete stop codon. There were 13 intergenic regions ranging 1 to 10 bp in size. Phylogenetically, T. leonina from a South China tiger was close to canine T. leonina. This study reports for the first time a complete mt genome sequence of T. leonina from the South China tiger, and provides a scientific basis for studying the genetic diversity of nematodes between different hosts.
Animals ; Animals, Wild ; Bias (Epidemiology) ; China* ; Codon, Initiator ; Codon, Terminator ; DNA, Intergenic ; Genes, rRNA ; Genetic Variation ; Genome ; Genome, Mitochondrial* ; Mammals ; Phylogeny ; RNA, Transfer ; Sequence Analysis* ; Tigers* ; Toxascaris*
Animals ; Animals, Wild ; Bias (Epidemiology) ; China* ; Codon, Initiator ; Codon, Terminator ; DNA, Intergenic ; Genes, rRNA ; Genetic Variation ; Genome ; Genome, Mitochondrial* ; Mammals ; Phylogeny ; RNA, Transfer ; Sequence Analysis* ; Tigers* ; Toxascaris*
The Korean Journal of Parasitology.2016;54(6):797-801. doi:10.3347/kjp.2016.54.6.797
Heishui county, located in northwest Sichuan province, southwestern China, is an endemic area of zoonotic visceral leishmaniasis (VL) and is the most intractable area. VL is never destroyed in it. Asymptomatic dogs (Leishmania parasites have been diagnosed but clinically healthy) are considered to be a potential reservoir host in zoonotic VL area, and most can lead to infection of individuals, that is a new challenge for controlling VL in humans. The present study aimed to assess the Leishmania infection rate of asymptomatic dogs in Heishui county. Total 105 asymptomatic domestic dogs were gathered from 4 districts in Heishui county to investigate the infection rate with serological and molecular methods based on ELISA and kinetoplast minicircle DNA(kDNA) PCR, respectively. Out of 105 dogs, 44 (41.9%) were positive by more than 1 method; 21 (20.0%) were positive by ELISA, and 30 (28.6%) were positive by kDNA-PCR. Our study showed that Leishmania infection of domestic dogs which is clinically healthy is prevalent in the studied district, and the asymptomatic dogs infected by Leishmania may be the primary reason for the prevalence of visceral leishmaniasis in the area.
Animals ; China* ; Dogs* ; Enzyme-Linked Immunosorbent Assay ; Epidemiology ; Humans ; Leishmania* ; Leishmaniasis, Visceral* ; Methods ; Parasites ; Polymerase Chain Reaction ; Prevalence
Animals ; China* ; Dogs* ; Enzyme-Linked Immunosorbent Assay ; Epidemiology ; Humans ; Leishmania* ; Leishmaniasis, Visceral* ; Methods ; Parasites ; Polymerase Chain Reaction ; Prevalence
A Case of Cerebral Cysticercosis in Thailand.
The Korean Journal of Parasitology.2016;54(6):793-795. doi:10.3347/kjp.2016.54.6.793
Cysticercosis and sparganosis are not uncommon parasitic infections in the developing world. Central nervous system infection by both cestodes can present with neurological signs and symptoms, such as seizure and mass effect, including brain hernia. Early detection and accurate diagnosis can prevent a fatal outcome. Histological examinations of brain tissues can confirm the diagnosis of cerebral cysticercosis, which differs from sparganosis by the presence of a cavitated body. We report here a case of cerebral cysticercosis which has the similar clinical and imaging findings as sparganosis.
Brain ; Central Nervous System Infections ; Cestoda ; Cysticercosis* ; Diagnosis ; Epilepsy ; Fatal Outcome ; Hernia ; Magnetic Resonance Imaging ; Neuropathology ; Seizures ; Sparganosis ; Thailand*
Brain ; Central Nervous System Infections ; Cestoda ; Cysticercosis* ; Diagnosis ; Epilepsy ; Fatal Outcome ; Hernia ; Magnetic Resonance Imaging ; Neuropathology ; Seizures ; Sparganosis ; Thailand*
The Korean Journal of Parasitology.2016;54(6):787-792. doi:10.3347/kjp.2016.54.6.787
Cutaneous leishmaniasis (CL) is a protozoan disease which is endemic in Iran. It is transmitted by the Phlebotomus sand fly. The eyelid is rarely involved possibly because the movement of the lids impedes the sand fly from biting the skin in this region. Here, we report 6 rare cases of eyelid CL. The patients were diagnosed by skin scraping, culture, and PCR from the lesions. Skin scraping examination showed Leishmania spp. amastigotes in the cytoplasm of macrophages. Culture examination was positive for Leishmania spp. PCR was positive for Leishmania major and Leishmania tropica. The lesions were disguised as basal cell carcinoma, chalazion, hordeolum, and impetigo. The patients were treated with intramuscular meglumine antimoniate (20 mg/kg/day) for at least 3 weeks. They showed a dramatic response, and the lesions almost completely disappeared. We emphasized the importance of clinical and diagnostic features of lesions, characterized the phylogenetic relationship of isolated parasites, and reviewed the literature on ocular leishmaniasis.
Carcinoma, Basal Cell ; Chalazion ; Cytoplasm ; Eyelids* ; Hordeolum ; Humans ; Impetigo ; Iran ; Leishmania ; Leishmania major ; Leishmania tropica ; Leishmaniasis ; Leishmaniasis, Cutaneous* ; Macrophages ; Meglumine ; Parasites ; Phlebotomus ; Polymerase Chain Reaction ; Psychodidae ; Skin
Carcinoma, Basal Cell ; Chalazion ; Cytoplasm ; Eyelids* ; Hordeolum ; Humans ; Impetigo ; Iran ; Leishmania ; Leishmania major ; Leishmania tropica ; Leishmaniasis ; Leishmaniasis, Cutaneous* ; Macrophages ; Meglumine ; Parasites ; Phlebotomus ; Polymerase Chain Reaction ; Psychodidae ; Skin
The Korean Journal of Parasitology.2016;54(6):777-786. doi:10.3347/kjp.2016.54.6.777
Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.
Agriculture ; Cestoda* ; Chickens* ; Eggs ; Microscopy, Electron, Scanning ; NAD ; Ovum ; Oxidoreductases ; Phylogeny* ; Public Health ; Thailand* ; Trees
Agriculture ; Cestoda* ; Chickens* ; Eggs ; Microscopy, Electron, Scanning ; NAD ; Ovum ; Oxidoreductases ; Phylogeny* ; Public Health ; Thailand* ; Trees
Factors Associated with Echinococcosis-Induced Perioperative Anaphylactic Shock.
The Korean Journal of Parasitology.2016;54(6):769-775. doi:10.3347/kjp.2016.54.6.769
This retrospective case-control study explored the factors associated with anaphylactic shock during surgery for cystic echinococcosis (CE) at the First Affiliated Hospital of Xinjiang Medical University between October 2008 and September 2013. Patients who suffered from anaphylactic shock (n=16) were age-matched 3:1 to patients who did not (n=43). Multivariate analysis suggested that IL-4 levels (odds ratio=1.096; 95% confidence interval=1.015–1.185; P=0.02) and cyst size (odds ratio=3.028, 95% confidence interval=1.259–7.283, P=0.013) were independently associated with CE-induced perioperative anaphylactic shock. Using the receiver operating characteristic (ROC) curves and a cut-off value of 415.7 ng/ml, IL-4 showed an area under the ROC (AUC) of 0.926, sensitivity of 75.0%, and specificity of 97.7%. Using a cut-off value of 7.8 cm, cyst size showed an AUC of 0.828, sensitivity of 81.3%, and specificity of 76.7%. In conclusion, results suggest that levels of IL-4 and cyst size were independently associated with echinococcosis-induced perioperative anaphylactic shock. These results could help identifying patients with echinococcosis at risk of anaphylactic shock in whom appropriate prophylaxis could be undertaken.
Anaphylaxis* ; Area Under Curve ; Case-Control Studies ; Echinococcosis ; Echinococcus granulosus ; Humans ; Interleukin-4 ; Multivariate Analysis ; Retrospective Studies ; ROC Curve ; Sensitivity and Specificity ; Zoonoses
Anaphylaxis* ; Area Under Curve ; Case-Control Studies ; Echinococcosis ; Echinococcus granulosus ; Humans ; Interleukin-4 ; Multivariate Analysis ; Retrospective Studies ; ROC Curve ; Sensitivity and Specificity ; Zoonoses
The Korean Journal of Parasitology.2016;54(6):759-768. doi:10.3347/kjp.2016.54.6.759
Cystic echinococcosis (CE) treatment urgently requires a novel drug. The p38 mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases, but still have to be characterized in Echinococcus granulosus. We identified a 1,107 bp cDNA encoding a 368 amino acid MAPK protein (Egp38) in E. granulosus. Egp38 exhibits 2 distinguishing features of p38-like kinases: a highly conserved T-X-Y motif and an activation loop segment. Structural homology modeling indicated a conserved structure among Egp38, EmMPK2, and H. sapiens p38α, implying a common binding mechanism for the ligand domain and downstream signal transduction processing similar to that described for p38α. Egp38 and its phosphorylated form are expressed in the E. granulosus larval stages vesicle and protoscolices during intermediate host infection of an intermediate host. Treatment of in vitro cultivated protoscolices with the p38-MAPK inhibitor ML3403 effectively suppressed Egp38 activity and led to significant protoscolices death within 5 days. Treatment of in vitro-cultivated protoscolices with TGF-β1 effectively induced Egp38 phosphorylation. In summary, the MAPK, Egp38, was identified in E. granulosus, as an anti-CE drug target and participates in the interplay between the host and E. granulosus via human TGF-β1.
Cloning, Molecular* ; DNA, Complementary ; Echinococcosis ; Echinococcus granulosus* ; Echinococcus* ; Humans ; In Vitro Techniques ; p38 Mitogen-Activated Protein Kinases ; Phosphorylation ; Phosphotransferases ; Protein Kinases* ; Signal Transduction
Cloning, Molecular* ; DNA, Complementary ; Echinococcosis ; Echinococcus granulosus* ; Echinococcus* ; Humans ; In Vitro Techniques ; p38 Mitogen-Activated Protein Kinases ; Phosphorylation ; Phosphotransferases ; Protein Kinases* ; Signal Transduction
Draft Genome of Toxocara canis, a Pathogen Responsible for Visceral Larva Migrans.
The Korean Journal of Parasitology.2016;54(6):751-758. doi:10.3347/kjp.2016.54.6.751
This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.
Adult ; B-Lymphocytes ; Base Composition ; Classification ; DNA ; Female ; Genome* ; Genomics ; Humans ; Larva Migrans, Visceral* ; Metabolism ; Mucins ; Peptides ; Protein Structure, Tertiary ; Receptors, Antigen, T-Cell ; RNA ; Ryanodine Receptor Calcium Release Channel ; T-Lymphocytes ; Toxocara canis* ; Toxocara* ; Transcriptome ; Ubiquitin-Protein Ligases
Adult ; B-Lymphocytes ; Base Composition ; Classification ; DNA ; Female ; Genome* ; Genomics ; Humans ; Larva Migrans, Visceral* ; Metabolism ; Mucins ; Peptides ; Protein Structure, Tertiary ; Receptors, Antigen, T-Cell ; RNA ; Ryanodine Receptor Calcium Release Channel ; T-Lymphocytes ; Toxocara canis* ; Toxocara* ; Transcriptome ; Ubiquitin-Protein Ligases
The Korean Journal of Parasitology.2016;54(6):743-750. doi:10.3347/kjp.2016.54.6.743
Mus musculus (Rodentia: Muridae) has generally been infected with a rodent hookworm Nippostrongylus brasiliensis. In this report, we present morphological and molecular identification of N. brasiliensis by light and scanning electron microscopy and PCR amplification of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the protein sequences encoded by cox1 gene, respectively. Despite the use of N. brasiliensis in many biochemistry studies from India, their taxonomic identification was not fully understood, especially at the species level, and no molecular data is available in GenBank from India. Sequence analysis of cox1 gene in this study revealed that the present specimen showed close identity with the same species available in GenBank, confirming that the species is N. brasiliensis. This study represents the first record of molecular identification of N. brasiliensis from India and the protein structure to better understand the comparative phylogenetic characteristics.
Ancylostomatoidea ; Animals ; Biochemistry ; Databases, Nucleic Acid ; Electron Transport Complex IV ; India* ; Mice* ; Microscopy, Electron, Scanning ; Nippostrongylus* ; Polymerase Chain Reaction ; Rodentia ; Sequence Analysis
Ancylostomatoidea ; Animals ; Biochemistry ; Databases, Nucleic Acid ; Electron Transport Complex IV ; India* ; Mice* ; Microscopy, Electron, Scanning ; Nippostrongylus* ; Polymerase Chain Reaction ; Rodentia ; Sequence Analysis
Country
Republic of Korea
Publisher
The Korean Society for Parasitology and Tropical Medicine
ElectronicLinks
http://e-sciencecentral.org/journals/162/Editor-in-chief
Jong-Yil Chai
cjy@snu.ac.kr
Abbreviation
The Korean Journal of Parasitology
Vernacular Journal Title
ISSN
0023-4001
EISSN
1738-0006
Year Approved
2007
Current Indexing Status
Currently Indexed
Start Year
Description
The Korean Journal of Parasitology is the official journal paperless, on-line publication after Vol. 53, 2015 of The Korean Society for Parasitology and Tropical Medicine. Abbreviated title is ‘Korean J Parasitol’. It was launched in 1963. It contains original articles, case reports, brief communications, reviews or mini-reviews, book reviews, and letters to the editor on parasites of humans and animals, vectors, host-parasite relationships, zoonoses, and tropical medicine. It is published bimonthly in February, April, June, August, October, and December each year. Supplement numbers are at times published. All of the manuscripts are peer-reviewed. Total or a part of the articles in this Journal are abstracted in Biological Abstracts, CAB International, Chemical Abstract, DOI/Crossref, Excerpta Medica (EMBASE), Google Scholar, Helminthological Abstracts, Index Medicus/Medline/PubMed/PubMed Central, KoMCI, KoreaMed, Protozoological Abstracts, Review of Medical and Veterinary Entomology, Science Citation Index Expanded, Scopus, Tropical Diseases Bulletin, WPRIM, and Zoological Record.
Current Title
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