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Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium.

So Yeong LEE ; Kyong Ah YOON ; Soo Hwa JANG ; Erdene Ochir GANBOLD ; Dembereldorj UURIINTUYA ; Sang Mo SHIN ; Pan Dong RYU ; Sang Woo JOO

Journal of Veterinary Science.2009;10(4):299-304. doi:10.4142/jvs.2009.10.4.299

The vibrational spectral differences of normal and lung cancer cells were studied for the development of effective cancer cell screening by means of attenuated total reflection infrared spectroscopy. The phosphate monoester symmetric stretching nus(PO3(2-)) band intensity at ~970 cm-1 and the phosphodiester symmetric stretching nus(PO2-) band intensity at ~1,085 cm-1 in nucleic acids and phospholipids appeared to be significantly strengthened in lung cancer cells with respect to the other vibrational bands compared to normal cells. This finding suggests that more extensive phosphorylation occur in cancer cells. These results demonstrate that lung cancer cells may be prescreened using infrared spectroscopy tools.
*Carcinoma ; Cell Line, Tumor ; Epithelial Cells/*physiology ; Humans ; *Lung Neoplasms ; Respiratory Mucosa/*cytology ; *Spectrophotometry, Infrared

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Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes.

Eunsong LEE ; Mohammad Musharraf Uddin BHUIYAN ; Hiroyuki WATANABE ; Kohji MATSUOKA ; Yoshihiro FUJISE ; Hajime ISHIKAWA ; Yutaka FUKUI

Journal of Veterinary Science.2009;10(4):285-292. doi:10.4142/jvs.2009.10.4.285

In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fetal whale fibroblasts with enucleated porcine oocytes were cultured in modified porcine zygote medium-3 to examine the effects of osmolarity and fetal serum on their in vitro developmental capacity. More than 66% of the whale somatic cells successfully fused with the porcine oocytes following electrofusion. A portion (60~81%) of the iSCNT whale embryos developed to the two- to four-cell stages, but no embryos were able to reach the blastocyst stage. This developmental arrest was not overcome by increasing the osmolarity of the medium to 360 mOsm or by the addition of fetal bovine or fetal whale serum. Our results demonstrate that sei whale-porcine hybrid embryos may be produced by SCNT using subzonal injection and electrofusion. The pig oocytes partly supported the remodeling and reprogramming of the sei whale somatic cell nuclei, but they were unable to support the development of iSCNT whale embryos to the blastocyst stage.
Animals ; Cloning, Organism/*veterinary ; Culture Media ; Embryo, Mammalian ; Karyotyping ; Nuclear Transfer Techniques/*veterinary ; *Oocytes ; Swine/*embryology ; Whales/*embryology

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Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury.

Hak Hyun RYU ; Ji Hey LIM ; Ye Eun BYEON ; Jeong Ran PARK ; Min Soo SEO ; Young Won LEE ; Wan Hee KIM ; Kyung Sun KANG ; Oh Kyeong KWEON

Journal of Veterinary Science.2009;10(4):273-284. doi:10.4142/jvs.2009.10.4.273

In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by epidural balloon compression: C group (no ASCs treatment as control), V group (vehicle treatment with PBS), and ASC group (ASCs treatment). ASCs or vehicle were injected directly into the injured site 1 week after spinal cord injury. Pelvic limb function after transplantation was evaluated by Olby score. Magnetic resonance imaging, somatosensory evoked potential (SEP), histopathologic and immunohistichemical examinations were also performed. Olby scores in the ASC group increased from 2 weeks after transplantation and were significantly higher than C and V groups until 8 weeks (p<0.05). However, there were no significant differences between the C and V groups. Nerve conduction velocity based on SEP was significantly improved in the ASC group compared to C and V groups (p < 0.05). Positive areas for Luxol fast blue staining were located at the injured site in the ASC group. Also, GFAP, Tuj-1 and NF160 were observed immunohistochemically in cells derived from implanted ASCs. These results suggested that improvement in neurological function by the transplantation of ASCs in dogs with spinal cord injury may be partially due to the neural differentiation of implanted stem cells.
Adipose Tissue/*cytology ; Animals ; Cell Differentiation ; Dog Diseases/pathology/*therapy ; Dogs ; Neurons/*cytology ; Spinal Cord Injuries/therapy/*veterinary ; Stem Cell Transplantation/*veterinary ; Stem Cells/*cytology/physiology

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Acupuncture treatment for idiopathic Horner's syndrome in a dog.

Sung Jin CHO ; Okjin KIM

Journal of Veterinary Science.2008;9(1):117-119. doi:10.4142/jvs.2008.9.1.117

A one-year-old female English Cocker Spaniel dog with idiopathic Horner's Syndrome is described. The specific clinical signs in this specimen were miosis, ptosis, enophthalmos, and prolapsed nictitans for 2 days following sudden onset. According to history taking, ophthalmic, neurological, and radiological examination, the patient was diagnosed with idiopathic Horner's syndrome. Manual acupuncture treatment was applied to the dog on local points two times in 2 days. The local acupoints were ST-4 (Di Chang) and GB-1 (Tong Zi Liao). The day after the initial acupuncture treatment, clinical signs related to idiopathic Horner's syndrome had almost disappeared. The day after the second treatment, specific clinical signs were completely absent. During this period, the dog did not receive any orthodox treatment. Thus, it is suggested that manual acupuncture might be an effective therapy for idiopathic Horner's syndrome.
Acupuncture Therapy/*veterinary ; Animals ; Dog Diseases/*therapy ; Dogs ; Female ; Horner Syndrome/therapy/*veterinary

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Hepatic encephalomyelopathy in a calf with congenital portosystemic shunt (CPSS).

Valeria Cafe MARCAL ; Anna OEVERMANN ; Tim BLEY ; Patrizia PFISTER ; Julien MICLARD

Journal of Veterinary Science.2008;9(1):113-115. doi:10.4142/jvs.2008.9.1.113

A 4-month-old female Holstein Friesian calf was referred to the Veterinary Teaching Hospital, University of Berne, Switzerland for evaluation of ataxia, weakness, apathy and stunted growth. Clinical examination revealed generalized ataxia, propioceptive deficits, decreased menace response and sensibility. Postmortem examination did not reveal macroscopic changes of major organs. Histologically, the brain and the spinal cord lesions were characterized by polymicrocavitation, preferentially affecting the white matter fibers at the junction of grey and white matter and by the presence of Alzheimer type II cells. The liver revealed lesions consistent with a congenital portosystemic shunt, characterized by increased numbers of arteriolar profiles and hypoplasia to absence of portal veins. The pathological investigations along with the animal history and clinical signs indicated a hepatic encephalomyelopathy due to a congenital portosystemic shunt.
Animals ; Cattle ; Cattle Diseases/*congenital ; Cerebrum/pathology ; Female ; Hepatic Encephalopathy/*veterinary ; Liver/pathology ; Portal System/*abnormalities

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Acute monoblastic leukemia in a FeLV-positive cat.

Kreangsak PRIHIRUNKIT ; Nual Anong NARKKONG ; Suntaree APIBAL

Journal of Veterinary Science.2008;9(1):109-111. doi:10.4142/jvs.2008.9.1.109

A 1.6-year-old male domestic short hair cat was brought to the Veterinary Medical Teaching Hospital, Kasetsart University, with signs of severe anemia, depression, and general lymph node enlargement. Complete blood count revealed leukocytosis and massive undifferentiated blasts. Testing for antibodies specific to feline leukemia virus (FeLV) was positive, and FeLV nucleic acid was confirmed by nested polymerase chain reaction. Base on cytochemistry and ultrastructure, the cat was diagnosed with acute monoblastic leukemia.
Animals ; Cat Diseases/*diagnosis/*virology ; Cats ; Leukemia Virus, Feline/*isolation & purification ; Leukemia, Monocytic, Acute/diagnosis/*veterinary/virology ; Male ; Osteoblasts/ultrastructure

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Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus).

Sang Tae SHIN ; Sung Keun JANG ; Hong Suk YANG ; Ok Keun LEE ; Yhong Hee SHIM ; Won Il CHOI ; Doo Soo LEE ; Gwan Sun LEE ; Jong Ki CHO ; Young Won LEE

Journal of Veterinary Science.2008;9(1):103-107. doi:10.4142/jvs.2008.9.1.103

This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drugreleasing insert (CIDR) for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were transferred on day 3 and 4 after removal of the insert. Recipient goats were deprived of feed for 48 h, then suspended in a laparotomy cradle at an angle of 45degrees. After obtaining a sufficient pneumoperitoneum, the laparoscope and forceps were inserted abdominally through 5 mm trocar sleeves. Examination of the ovaries and uterus was performed and then 213 embryos were transferred into the oviducts via the infundibula of 76 recipient goats. To compare pregnancy rates, ET was also performed by laparotomy in 82 recipient goats. The pregnancies in the recipient goats were diagnosed by ultrasound on day 30 after embryo transfer. The pregnancy rate with laparoscopic ET was significantly higher than with ET performed by laparotomy (46.1% vs. 28.6%, p < 0.05). In addition, the pregnancy rates were compared between ovulated and non-ovulated ovaries of the recipient goats in the laparoscopic ET group. No significant difference was observed between the pregnancy rates of ovulated and non-ovulated ovaries (41.3% vs. 33.3%, p < 0.05) suggesting that ET may also be possible in non-ovulated recipients through artificial rupture of Graafian follicles. These results suggest that laparoscopic ET is a highly efficient method for the transfer of goat embryos.
Animals ; Animals, Genetically Modified/*embryology ; Embryo Transfer/methods/*veterinary ; Female ; Goats/*genetics/physiology ; Laparoscopy/*veterinary ; Laparotomy/*veterinary ; Microinjections/veterinary ; Oocytes

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Luteal lifespan and fertility after estrus synchronization in goats.

Lu Meng CHAO ; Koji TAKAYAMA ; Yoshitaka NAKANISHI ; Katsumi HAMANA ; Mitsuhiro TAKAGI ; Chikara KUBOTA ; Toshiyuki KOJIMA

Journal of Veterinary Science.2008;9(1):95-101. doi:10.4142/jvs.2008.9.1.95

The present experiment aims to examine the efficiency of estrus synchronization using progesterone and equine chorionic gonadotrophin (eCG) and to look at luteal function. During the non-breeding and breeding season, 5 adult female Korean native goats were injected intramuscularly with 2.5 ml of physiological saline as the control. A progesterone impregnated intravaginal sponge was then kept in the same goats for 10 days followed, after a week, by an intramuscular injection of 500 IU eCG. Five adult female Nubian goats were mated with a fertile buck during the non-breeding season. During the non-breeding season 2 of the 5 goats showed a normal estrous cycle (ranging from 18 to 21 days) and 3 a short estrous cycle (ranging from 3 to 6 days). During the breeding season the equivalent figures were 1 and 2. The major axes of the corpus luteum (CL) were measured by means of calipers built into the ultrasonography system, and the concentrations of plasma progesterone (P(4)) were determined by double antibody radioimmunoassay. The mean major axes of the CL in goats showing the short cycle (6.1 +/- 0.5 mm) was significantly smaller than in those showing the normal cycle (8.9 +/- 0.5 mm; p < 0.01) and also the value of P4 in goats showing the short cycle (4.2 +/- 2.1 ng/ml) was significantly lower than for those showing the normal cycle (10.3 +/- 4.3 ng/ml; p < 0.05) at day 3 following ovulation. Three out of 5 Nubian goats became pregnant but only one goat carried to full term. The present experiment indicated that a combination of progesterone and eCG was effective in inducing estrus, although it resulted in a high incidence of short luteal lifespan. The low kidding rate and high incidence of embryonic loss may be due to the instability of the luteal lifespan.
Animals ; Chorionic Gonadotropin/*pharmacology ; Corpus Luteum/*drug effects/*physiology ; Estrus Synchronization/*drug effects/physiology ; Female ; Fertility/*drug effects ; Fertility Agents, Female/pharmacology ; Goats/*physiology ; Horses ; Pregnancy ; Progesterone/blood/*pharmacology

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The Safety and efficacy of a new self-expandable intratracheal nitinol stent for the tracheal collapse in dogs.

Joon young KIM ; Hyun jung HAN ; Hun young YUN ; Bora LEE ; Ha young JANG ; Ki dong EOM ; Hee myung PARK ; Soon wuk JEONG

Journal of Veterinary Science.2008;9(1):91-93. doi:10.4142/jvs.2008.9.1.91

To evaluate the potential utility of a self-expandable intratracheal nitinol stent with flared ends for the treatment of tracheal collapse in dogs, endotracheal stenting therapy was performed under fluoroscopic guidance in four dogs with severe tracheal collapse. During the 4 to 7 month followup, after stent implantation, clinical signs, including dyspnea and respiratory distress, dramatically improved in all dogs. The radiographs showed that the implanted stents improved the tracheal collapse, and there were no side effects such as collapse, shortening or migration of the stents. In conclusion, the self-expandable intratracheal nitinol stents provided adequate stability to the trachea and were effective for attenuating the clinical signs associated with severe tracheal collapse.
*Alloys ; Animals ; Dog Diseases/*surgery ; Dogs ; Female ; Male ; Prosthesis Implantation/adverse effects/veterinary ; Stents/adverse effects/*veterinary ; Tracheal Stenosis/surgery/*veterinary

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Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea.

Seong Joon JOH ; Chae Ik SHON ; Sung Won KANG ; Byoung Han KIM ; Byung Yul JEONG ; Kyung Gi LEE ; Jun Hun KWON ; Gang Jun HEO

Journal of Veterinary Science.2008;9(1):85-90. doi:10.4142/jvs.2008.9.1.85

The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2,538 bp open reading frame, which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser/Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1 contains the Leu-Lys-Asn (LKN) motif instead of the typical Gly-Asp- Asp (GDD) motif found in other aquatic birnaviruses. We also identified the GLPYIGKT motif, the putative GTPbinding site at amino acid position 248. In total, the VP1 regions of 22 birnavirus strains were compared for analyzing the genetic relationship among the family Birnaviridae. Based on the deduced amino acid sequences, GC1 was observed to be more closely related to the infectious pancreatic necrosis virus (IPNV) from the USA, Japan, and Korea than the IPNV from Europe. Further, aquatic birnaviruses containing GC1 and IPNV have genogroups that are distinct from those in the genus Avibirnaviruses and Entomo-birnaviruses. The birnavirusstrains were clustered into 5 genogroups based on their amino acid sequences. The marine aquatic birnaviruses (MABVs) containing GC1 were included in the MABV genogroup; the IPNV strains isolated from Korea, Japan, and the USA were included in genogroup 1 and the IPNV strains isolated primarily from Europe were included in genogroup 2. Avibirnaviruses and entomobirnaviruses were included in genogroup 3 and 4, respectively.
Amino Acid Sequence ; Animals ; Base Sequence ; Birnaviridae/classification/*genetics ; Capsid Proteins/chemistry/*genetics ; Cell Line ; Fishes/*virology ; Korea ; Molecular Sequence Data ; Phylogeny

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