| 1. The analysis of heterogeneity of HWTX-I expressed in Pichia pastoris. |
Page:215—219 |
| 2. Introduce Tagsk1 into salt-sensitive callus to improve the capacity of salt-tolerance by micropartical bombardment. |
Page:211—214 |
| 3. Generation of selectable marker-free and vector backbone sequence-free Xa21 transgenic rice. |
Page:204—210 |
| 4. The changes of rare codon and mRNA structure accelerate expression of qa-3 in Escherichia coli. |
Page:198—203 |
| 5. Dual promoters enhance heterologous enzyme production from bacterial phage based recombinant Bacillus subtilis. |
Page:191—197 |
| 6. High-level expression of phenylalanine ammonia-lyase in Lactococcus Lactis via synthesized sequence based on bias codons. |
Page:187—190 |
| 7. Use of Ssp dnaB mini-intein as a fusion partner for preparation of recombinant human brain natriuretic peptide. |
Page:180—203 passim |
| 8. Expression in Pichia pastoris and properties of human serum albumin-interferon alpha2b chimera. |
Page:173—179 |
| 9. Application of microfluidic-chip in biomedicine. |
Page:167—171 |
| 10. The effect of glutamine on the growth, metabolism and endostatin production of microencapsulated rCHO cells. |
Page:162—166 |
| 11. Effect of osmotic pressure on nitrification. |
Page:156—161 |
| 12. Preparation of uniform-sized chitosan microspheres and application as carriers for protein drugs. |
Page:150—155 |
| 13. Modelling a penicillin fed-batch fermentation using least squares support vector machines. |
Page:144—149 |
| 14. Stimulatory effects of some amino acids on glycerol production by Candida glycerinogenes. |
Page:138—143 |
| 15. Construction of sugar-based microbial fuel cells by dissimilatory metal reduction bacteria. |
Page:131—137 |
| 16. Screening and detecting of proteins interacting with mPem. |
Page:125—130 |
| 17. Studies on phage displayed mimotopes of a protective monoclonal antibody (SSj14) against Schistosoma japonicum. |
Page:119—124 |
| 18. Immobilization of lipase on macroporous resin and its application in synthesis of biodiesel in low aqueous media. |
Page:114—118 |
| 19. Hairy root induction and plant regeneration of crownvetch (Coronilla varia L.) transformed by Agrobacterium rhizogenes. |
Page:107—113 |
| 20. Effects of hydrodynamic on aggregates formation, growth and metabolism of HEK293 cells in suspension culture. |
Page:101—106 |
| 21. Metabolism of recombinant CHO-GS cell reducing of toxic effect of ammonia. |
Page:94—100 |
| 22. High level expression, purification and cytotoxicity of IL-10(18-57)-PE40. |
Page:87—93 |
| 23. Recombinant Aspergillus niger glucose oxidase expressed in Trichoderma reesei. |
Page:82—86 |
| 24. Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis. |
Page:77—81 |
| 25. High-level production of neutral endoglucanase 1 in Pichia pastoris. |
Page:71—76 |
| 26. Acute toxicity and immunoprotection of recombinant apxI toxin of Actinobacillus pleuropneumoniae in mice. |
Page:65—70 |
| 27. Prokaryotic expression of functional PTEN in Escherichia coli and preparation of polyclonal antibody. |
Page:58—64 |
| 28. Molecular cloning of tomato LeHsp110/ClpB gene and its effect on the thermotolerance in plant. |
Page:52—57 |
| 29. Cloning, soluble expression and characterization of human sBCMA. |
Page:46—51 |
| 30. Study on immunogenicity of the N-terminal polypeptide of RTX toxin I of Actinobacillus pleuropneumoniae. |
Page:39—45 |
| 31. Functional expression of an omega-3 fatty acid desaturase gene from Glycine max in Saccharomyces cerevisiae. |
Page:33—38 |
| 32. Improvement of the thermostability of xylanase by N-terminus replacement. |
Page:26—32 |
| 33. Large recombinant protein displayed on filamentous phage surface and its interaction with small molecule. |
Page:19—25 |
| 34. Development and challenge of modified hemoglobins as red blood cell substitutes. |
Page:7—18 |
| 35. Taxol-producing fungi: a new approach to industrial production of taxol. |
Page:1—6 |
| 36. Effect of homogeneity on cell growth and anthocyanin biosynthesis in suspension cultures of Vitis vinifera. |
Page:805—810 |
| 37. Production of gastrodin through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of Datura tatula L. |
Page:800—804 |
| 38. Preparation of new lipid-hydroxyapatite-DNA complex and gene transfection reseach in eukaryotic cell. |
Page:795—799 |
| 39. Rapid detection of Pseudomonas aeruginosa by the fluorescence quantitative PCR assay targeting 16S rDNA. |
Page:789—794 |
| 40. Transgenic mice produced by intracytoplasmic sperm injection. |
Page:784—788 |
| 41. Gene construction, expression and activities analysis of human leptin. |
Page:779—783 |
| 42. The effect of human IL-17F on growth of human hepatocarcinoma xenograft tumor in nude mice. |
Page:772—778 |
| 43. The synthesis and function analysis of omega-3 fatty acid desaturase gene from Caenorhabditis briggssae. |
Page:763—771 |
| 44. Activation of silent antibiotic synthesis in Streptomyces lividans by disruption of a negative regulator nsdA, a gene conserved in Streptomyces. |
Page:757—762 |
| 45. Gene cloning and expression analysis of a male sterility related gene BoDHAR from broccoli. |
Page:751—756 |
| 46. Produce of marker-free transgenic tobacco plants by FLP/frt recombination system. |
Page:744—750 |
| 47. Cloning, prokaryotic expression of chicken interferon-alpha gene and study on antiviral effect of recombinant chicken interferon-alpha. |
Page:737—743 |
| 48. Fusion expression of O type foot-and-mouth diseases virus VP1 gene and HSP70 gene and induction of immune responses in mice. |
Page:733—736 |
| 49. Cloning and analysis of phage Fab antibodies of mouse male specific antigen. |
Page:727—732 |
| 50. Generation high yield vaccine strain wholly derived from avian influenza viruses by reverse genetics. |
Page:720—726 |
| 51. The construction of recombinant adenovirus expressing bifunctional fusion protein sCAR-EGF and the detection of its activity. |
Page:713—719 |
| 52. Organization and transcription strategy of genome of Bombyx mori bidensovirus (China isolate) VD1. |
Page:707—712 |
| 53. Identification of mimotope peptides which bind to the SARS-CoV spike protein specific monoclonal antibody 2C5 with phage-displayed peptides library. |
Page:701—706 |
| 54. Progresses in the structure and function of Kazal-type proteinase inhibitors. |
Page:695—700 |
| 55. Studies on spermatogonial stem cells cultured in vitro of Wuzhishan Mini Porcine. |
Page:689—693 |
| 56. Screen of media for insect cells (HzAm1) growth and its adaptation to low serum culture. |
Page:686—688 |
| 57. Molecular cloning of an amidase gene from Nocardia sp. and its expressionin Escherichia coli. |
Page:682—685 |
| 58. Porcine follistatin cDNA cloning and expression in Escherichia coli. |
Page:677—681 |
| 59. Three-dimensional spheroid model for cultivating WB-F344 cells in simulated microgravity. |
Page:672—676 |
| 60. The effect of NAD kinase homologues on the beta-oxidation of unsaturated fatty acids with the double bond at an even position in Saccharomyces cerevisiae. |
Page:667—671 |
| 61. Hydroxylation of 16alpha, 17alpha-epoxy-4-pregenene-3, 20-dione by Absidia coerulea with pseudo-crystallo feed. |
Page:662—666 |
| 62. Optimization of SRAP & ISSR technology and its application in the identification of seeds of Brassica oleracea L. |
Page:657—661 |
| 63. Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides. |
Page:650—656 |
| 64. Isolation and identification of lipopeptides produced by Bacillus subtilis fmbJ. |
Page:644—649 |
| 65. Purification and biochemical character of a fibrinolytic protein from Eupolyphaga sinensis. |
Page:639—643 |
| 66. Purification and properties of isoflavone-glucosidase. |
Page:635—638 |
| 67. Production of polyclonal and monoclonal antibodies against citrus tristeza virus and their efficiency for the detection of the virus. |
Page:629—634 |
| 68. Effects of cu2+ on biosynthesis of camptothecin in cell cultures of Camptotheca acuminata. |
Page:624—628 |
| 69. Effects of exogenous phytohormones on hairy root growth and biosynthesis of anthraquinones in the hairy root culture of Polygonum multiflorum. |
Page:619—623 |
| 70. Effect of phosphate on growth and polysaccharide production by suspension cultures of protocorm-like bodies of Dendrobium huoshanense. |
Page:613—618 |
| 71. Studies on relationship between the expression of hTNF-alpha gene and photosynthesis in Anabaena sp. IB02. |
Page:609—612 |
| 72. Simultaneous expression of modified hepatitis B surface antigen fusion polypeptides containing preS1, preS2 epitopes in Pichia pastoris. |
Page:604—608 |
| 73. Prokaryotic expression of fusion protein TAT-EDAG and study on its transduction activity. |
Page:598—603 |
| 74. Cloning and expression of pokeweed antiviral protein-II gene from the summer leaves of Phytolacca amercana. |
Page:592—597 |
| 75. Cloning of distinguishing DNA sequences of Gastrodia elata Blume and application of them in identifying gastrodia tuber. |
Page:587—591 |
| 76. A method using long primers for cloning the upstream sequence of delta-6 fatty acid desaturases gene of Thamnidium elegans by nested inverse PCR. |
Page:581—586 |
| 77. Improvement of baculovirus expression system and purification of IL-6 protein expressed in insect cells. |
Page:572—580 |
| 78. Overproduction of glucoamylase by recombinant Aspergillus niger harboring multiple copies of glaA. |
Page:567—571 |
| 79. Cloning and expression of Oreochromis aurea gonadotropin-releasing hormone cDNA in Escherichia coli and its immunogenicity for mice. |
Page:561—566 |
| 80. Construction and immunogenicity of recombinant adenovirus tandem expressing M and GP5 of porcine reproductive and respiratory syndrome virus. |
Page:555—560 |
| 81. A novel binary vector to get marker-free transgenic plant. |
Page:550—554 |
| 82. Expression of the gene coding for a thermostable alpha-amylase from Pyrococcus furious in Chiamydomonas reinhardtii chloroplast . |
Page:545—549 |
| 83. SCT-OGP fusion protein expression and its potential role as osteoporosis therapy. |
Page:539—544 |
| 84. Knockout of the hprK gene in B. subtilis CcpA mutant and its influence on riboflavin fermentation. |
Page:534—538 |
| 85. Improving phytase expression by increasing the gene copy number of appA-m in Pichia pastoris. |
Page:528—533 |
| 86. Fusion tags technology and their applications. |
Page:523—527 |
| 87. Induction of cucumber hairy roots and effect of cytokinin 6-BA on its growth and morphology. |
Page:514—520 |
| 88. Study on ethanol tolerance of Saccharomyces cerevisiae X330 under very high gravity medium. |
Page:508—513 |
| 89. A new purification methods of small DNAs--the purification methods with silica wool. |
Page:504—507 |
| 90. Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris. |
Page:499—503 |
| 91. Construction of recombinant yellow fever virus 17D containing 2A fragment as a vaccine vector. |
Page:492—498 |
| 92. Expression, purification and activity analysis of anti-human transferrin receptor scFv. |
Page:488—491 |
| 93. Fermentation process monitoring and fault detection based on dynamic MPCA. |
Page:483—487 |
| 94. Preparation and identification of soluble trimeric CD40-isoleucine zipper fusion protein. |
Page:477—482 |
| 95. Construction, expression and preliminary pharmacokinetic analysis of IL-1ra mutants. |
Page:472—476 |
| 96. Effect of sugars, gibberellic acid and abscisic acid on somatic embryogenesis in Tylophora indica (Burm. f.) Merrill. |
Page:465—471 |
| 97. Preparation of the cDNA microarray on the differential expressed cDNA of senescence-accelerated mouse's hippocampus. |
Page:457—464 |
| 98. Expression of human tumstatin in Pichia pastoris and its bioactivity. |
Page:451—456 |
| 99. Cloning of F gene of Newcastle disease virus HeB02 isolate and the study of its DNA vaccine. |
Page:445—450 |
| 100. Study on molecular identification and pre-harvest sprouting characteristic of the transgenic anti-trxs-gene wheat line 00T89. |
Page:438—444 |
| 101. Transgenic wheat (Triticum aestivum L.) with increased resistance to the storage pest obtained by Agrobacterium tumefaciens--mediated. |
Page:431—437 |
| 102. Construction of the eukaryotic expression vector with IL-2 gene and VP2 gene of PPV and research on immunogenicity. |
Page:425—430 |
| 103. Study of fusion protein and attachment glycoprotein of Nipah virus expressed in recombinant baculovirus. |
Page:418—424 |
| 104. Cloning and expression of human interleukin-26 in Escherichia coli. |
Page:413—417 |
| 105. Molecular cloning, tissue distribution and expression in engineered cells of human orphan receptor GPR81. |
Page:408—412 |
| 106. Analysis of the relationship between nm23-H1 gene and human chronic myeloblastic leukemia using siRNA. |
Page:403—407 |
| 107. The effect of oncolytic adenovirus on human umbilical vein endothelial cell. |
Page:397—402 |
| 108. Construction and immunological characterization of recombinant Marek's disease virus expressing IBDV VP2 fusion protein. |
Page:391—396 |
| 109. In vitro cytolysis of B-lymphoma cells mediated by an anti-CD3/anti-CD20 bispecific single-chain antibody. |
Page:384—390 |
| 110. Expression of sTNFR-IgGFc fusion gene in endothelial cell and its application in gene therapy for rheumatoid arthritis. |
Page:378—383 |
| 111. Construction and expression of various human prion proteins mutants with modified N-glycosylation sites in mammalian cells. |
Page:373—377 |
| 112. Construction and characterization of delta crp delta asd mutant host-vector balanced lethal system of Salmonella choleraesuis C500 strain. |
Page:366—372 |
| 113. Properties and advance of hepcidin. |
Page:361—365 |
| 114. Application of glycosyl fluorides in the study on glycosidases. |
Page:351—360 |
| 115. DC conductivity of DNA solution. |
Page:334—337 |
| 116. A review on genetic testing. |
Page:338—343 |
| 117. Inhibitory effect of new antimicrobial substance by Bacillus subtilis fmbJ on Newcastle disease virus and infectious Bursal disease virus in vitro. |
Page:328—333 |
| 118. Research of the feeding strategy in the fermentation of recombinant human fibreblast growth factor mutant. |
Page:322—327 |
| 119. Effects of temperature on the kinetics and level of energy charge and oxidation-reduction state in pyruvate biosynthesis. |
Page:316—321 |
| 120. The effect of induction temperature on aggregation of consensus interferon-alpha expressed by Pichia pastoris. |
Page:311—315 |
| 121. The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos. |
Page:306—310 |
| 122. Significant improved anthocyanins biosynthesis in suspension cultures of Vitis vinifera by process intensification. |
Page:299—305 |
| 123. A study on the discrimination of thermophilic and mesophilic proteins based on dipeptide composition. |
Page:293—298 |
| 124. Preparation of recombinant HIV-TATm-survivin (T34A) protein and its pro-apoptosis activity to cancer cells in vitro. |
Page:285—292 |
| 125. Development of a new hydrophobic interaction chromatography absorbent and its application to the purification of recombinant hepatitis B surface antigen. |
Page:278—284 |
| 126. Detection of intracellular reactive oxygen species by flow cytometry in Pichia pastoris fermentation. |
Page:273—277 |
| 127. Effect of feeding pre-L-methionine on high-cell-density fermentation for S-adenosyl-L-methionine production. |
Page:268—272 |
| 128. Study on the asexual sporulation of Aspergillus niger under blue light induction and analysis of its subtractive library. |
Page:263—267 |
| 129. Effect of straw apoplast protein on cellulase activity. |
Page:257—262 |
| 130. Recombinant expression and secretion of mpd gene using the promoters and signal peptide-encoding sequences of ytkA and ywoF gene from Bacillus subtilis. |
Page:249—256 |
| 131. Construction of human growth hormone lentiviral vector and its expression in murine skeletal myoblasts. |
Page:243—248 |
| 132. Molecular cloning and expression of extremely thermostable and acid-stable amylase gene in Pichia pastoris. |
Page:237—242 |
| 133. Expression of AIV subtype H5HA, H7HA and H9HA hemagglutinin gene in Pichia pastoris. |
Page:231—236 |
| 134. Expression of human LNalpha4LG4-5 in Pichia yeast. |
Page:226—230 |
| 135. Construction of VEGF recombinant plasmid pcDNA/V and its expression in model rats with acute myocardial ischemia. |
Page:220—225 |
| 136. Comparative study on in vitro stationary culture system or superfusion culture system of cow mammary tissue. |
Page:1040—1046 |
| 137. Expression of porcine beta-defensin 1 gene in Pichia pastoris. |
Page:1036—1039 |
| 138. Design and implementation of DB sequence optimization software. |
Page:1032—1035 |
| 139. Application of boosting-based decision tree ensemble classifiers for discrimination of thermophilic and mesophilic proteins. |
Page:1026—1031 |
| 140. The influences of lactose as an inducer on expression of plant des-pGlu1-brazzein in Escherichia coli. |
Page:1021—1025 |
| 141. Study on application of rep-PCR fingerprint in rapid identification of beer-spoilager. |
Page:1013—1020 |
| 142. Methane monooxygenases hydroxylase from a type II methanotroph: purification and physical-chemical properties. |
Page:1007—1012 |
| 143. Effects of baicalein on the proliferation and differentiation of pig preadipocyte. |
Page:1002—1006 |
| 144. Expression and characterization of VPAC2 in CHO cells. |
Page:996—1001 |
| 145. Establishment and application of human papillomavirus type 16 pseudovirions neutralization assay. |
Page:990—995 |
| 146. Impact of subculture cycles and inoculum sizes on suspension cultures of Vitis vinifera. |
Page:984—989 |
| 147. Selecting the RNAi efficiency target sites of lZP3 gene by mouse in vivo. |
Page:979—983 |
| 148. Expression of single chain fragment variable P1D3 antibody against shrimp white spot syndrome virus in Pichia pastoris. |
Page:973—978 |
| 149. Expression, characterization and biological activity analysis of recombinant human bone morphogenetic protein 2 in CHO cells. |
Page:968—972 |
| 150. Expression and purification of recombinant human interleukin 4 in Escherichia coli. |
Page:962—967 |
| 151. Expression of recombinant human cytomegalovirus fusion proteins pp150/MDBP fragments and its application. |
Page:956—961 |
| 152. Cloning and expression of the genes encoding glycerol dehydratase reactivase and identification of its biological activity. |
Page:950—955 |
| 153. Isolation, sequencing analysis and characterization of the promoter of banana lectin gene. |
Page:945—949 |
| 154. Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis. |
Page:940—944 |
| 155. Construction and characterization of a recombinant fowlpox virus co-expressing F, HN genes of Newcastle disease virus and gB gene of infectious laryngnotracheitis virus. |
Page:931—939 |
| 156. The study of the growth-suppression and mechanisms of hepatocelluar carcinoma tumor in nude mice. |
Page:925—930 |
| 157. New option for gene-targeting vector construction Red/ET recombination. |
Page:919—924 |
| 158. Construction and expression of HSV-2gD-Hsp70 fusion protein gene. |
Page:914—918 |
| 159. Expression and secretion of human bone morphogenetic protein-7 in Pichia pastoris. |
Page:907—913 |
| 160. Cloning and analysis of geldanamycin partial biosynthetic gene cluster of Streptomyces hygroscopicus 17997. |
Page:902—906 |
| 161. In silico cloning of Efp-0, a novel earthworm fibrinolytic enzyme gene and verification of its coding region by RT-PCR. |
Page:897—901 |
| 162. Advances in the RNA-directed DNA methylation in plants. |
Page:891—896 |
| 163. Specific promoters used in plant gene engineering. |
Page:882—890 |
| 164. Advances in heavy metal ions immunoassay. |
Page:877—881 |
| 165. Research progress in SELDI-TOF MS and its clinical applications. |
Page:871—876 |
| 166. The advancement of AFLP technology. |
Page:861—865 |
| 167. Application of optical proteinchip in detecting phage M13KO7. |
Page:856—860 |
| 168. Effects of resveratrol on pig primary preadipocytes proliferation, differentiation and transcription expression of Sirt1 gene. |
Page:850—855 |
| 169. Study on vitrification of porcine embryos by open pulled straw method. |
Page:845—849 |
| 170. Process performance of simultaneous anaerobic sulfide and nitrate removal. |
Page:840—844 |
| 171. Isolation and characterization of gamma-TMT gene promoter from Arabidopsis thaliana. |
Page:835—839 |
| 172. The preparation of an elicitor from a fungal endophyte to enhance artemisinin production in hairy root cultures of Artemisia annua L. |
Page:829—834 |
| 173. Model study on CO2 removal by photobioreactor. |
Page:821—828 |
| 174. Continuous ethanol fermentation coupled with recycling of yeast flocs. |
Page:816—820 |
| 175. Refolding of the fusion protein of recombinant enterokinase light chain rEKL. |
Page:811—815 |
