| 1. Filter-based template preparation for rapid and sensitive PCR detection of Staphylococcus aureus in meat. |
Page:1009—1013 |
| 2. Effects of sucrose and light on the growth and production of secondary metabolites in Pueraria phaseoloides hairy roots. |
Page:1003—1008 |
| 3. Determination of the catalytic structures of methyl parathion hydrolase. |
Page:998—1002 |
| 4. Purification and renaturation of recombinant human Cu, Zn-SOD by metal-chelating affinity chromatography. |
Page:993—997 |
| 5. Biological synthesis of L-ascorbyl palmitate. |
Page:988—992 |
| 6. Improving thermostability of Aspergillus niger phytase by elongation mutation. |
Page:983—987 |
| 7. CDNA microarray on differentially expressed genes of adipose tissue in two breeds chicken. |
Page:979—982 |
| 8. The expression of porcine circovirus type 2 ORF2 gene in insect cells and its character. |
Page:975—978 |
| 9. Isolation and functional analysis of tobacco MARs. |
Page:970—974 |
| 10. Study on rhG-CSF modified with polyethylene glycol. |
Page:965—969 |
| 11. A study on the pattern recognition of thermophilic and mesophilic proteins. |
Page:960—964 |
| 12. Screening of a triazophos-degrading strain and pathway of its degradation. |
Page:954—959 |
| 13. Polyethylene glycol-accompanied ion-exchange chromatography to purify recombinant hepatitis B virus surface antigen. |
Page:947—953 |
| 14. Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production. |
Page:942—946 |
| 15. Comparative proteomic analysis of cerebral cortex between middle cerebral artery occlusion rats and normal controls. |
Page:934—941 |
| 16. Purification of a ginsenoside-Rb1 hydrolase from Helix snailase. |
Page:929—933 |
| 17. Characteristics of cell cycle and metabolism in microencapsulated K562 cell culture. |
Page:923—928 |
| 18. Establishment and optimization of in vitro regeneration system for Plantago major L. |
Page:916—922 |
| 19. High level expression of PNGase F in Escherichia coli and its bioactivities. |
Page:911—915 |
| 20. Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis). |
Page:906—910 |
| 21. Construction and screening of phage display single chain antibody library against Bursaphelenchus xylophilus cellulase. |
Page:900—905 |
| 22. Cloning and expressing of cellulase gene (cbh2) from thermophilic fungi Chaetomium thermophilum CT2. |
Page:892—899 |
| 23. Construction of multifunctional genetically engineered pesticides-degrading bacteria by homologous recombination. |
Page:884—891 |
| 24. Expression of endo-beta-mannanase gene from Trichoderma reesei in Pichia pastoris. |
Page:878—883 |
| 25. Heteroexpression of Rhizopus arrhizus delta6-fatty acid desaturase gene in Pichia pastoris. |
Page:871—877 |
| 26. Salmonella choleraesuis C500 delivering DNA immunization against classical swine fever virus. |
Page:865—870 |
| 27. Construction and immunogenicity of recombinant pseudorabies virus expressing the modified GP5m protein of porcine reproduction and respiratory syndrome virus. |
Page:858—864 |
| 28. Genomic DNA sequences and functional expression, purification of BmalphaTX14 neurotoxin from scorpion Buthus martensii Karsch. |
Page:853—857 |
| 29. Study on breeding up high-yield strain of taxol by protoplast mutagensis. |
Page:848—851 |
| 30. Effects of CO2 on high density culture of Chaetoceros mulleri. |
Page:844—847 |
| 31. Effects of docosahexaenoic acid on rat adipocytes proliferation and differentiation. |
Page:840—843 |
| 32. The expression of VP19 gene from prawn white spot syndrome virus in silkworm, Bombyx mori using host range-expanded HyNPV. |
Page:837—839 |
| 33. Construction of a recombinant baculovirus transfer vector with two promoters expressing the anti-human CD28 chimeric antibody by using TP-PCR method. |
Page:832—836 |
| 34. Expression of ATR-Fc fusion protein in CHO cells. |
Page:826—831 |
| 35. Study on energy dissipation in modified airlift bioreactor. |
Page:820—825 |
| 36. Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology. |
Page:814—819 |
| 37. Protein amino acid composition of plasma membranes affects membrane fluidity and thereby ethanol tolerance in a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. |
Page:809—813 |
| 38. Expression of fusion protein of parathyroid hormone and transferrin N-terminal half-molecule in Pichia pastoris. |
Page:804—808 |
| 39. Kinetics model of spherical immobilized cellulase. |
Page:799—803 |
| 40. High expression of mirobial transglutaminase gene from Streptoverticillium mobaraense in Escherichia coli. |
Page:794—798 |
| 41. Oriented immobilization of papain on metal chelating carriers. |
Page:789—793 |
| 42. Gene cloning and expression of serine protease SFP2 from Streptomyces fradiae var. k11. |
Page:782—788 |
| 43. Directed evolution of D-lactonohydrolase by error prone PCR and DNA shuffling. |
Page:773—781 |
| 44. Cryopreservation of mouse embryos in ethylene glycol-based solutions: a search for the optimal and simple protocols. |
Page:766—772 |
| 45. Establishment of a cell model targeted to NFAT signal transduction pathway for preliminary screening of FK506-like immunosuppressants. |
Page:759—765 |
| 46. The growth characteristics and ginsenosides isolation of suspension-cultured crown gall of Panax quinquefolium. |
Page:754—758 |
| 47. Construction of a trans-splicing ribozyme for restoring EGFP truncation mutation. |
Page:748—753 |
| 48. Construction of novel recombinant Escherichia coli capable of producing 1,3-propanediol. |
Page:743—747 |
| 49. Sequence analysis of alpha-amylase inhibitors genes with resistance to insects in wheat and Aegilops. |
Page:737—742 |
| 50. Secreted expression of porcine interferon-gamma gene in Pichia pastoris. |
Page:731—736 |
| 51. Immunogenicity of DNA vaccine expressing GP5 of porcine reproductive and respiratory syndrome virus fused with VP22 of bovine herpesvirus 1. |
Page:725—730 |
| 52. The expression of recombinant protein of human interleukin-24 and its anti-tumor mechanism. |
Page:719—724 |
| 53. Construction of DNA and RNA based on bifunctional replicon vector derived from Semliki Forest virus. |
Page:713—718 |
| 54. The purification of HBV full-length PreS protein in Pichia pastoris. |
Page:708—712 |
| 55. Cloning and expression of the extracellular domain of 4-1BBL. |
Page:703—707 |
| 56. Homodimerization of the c-Abl protein tyrosine kinase. |
Page:698—702 |
| 57. Expression and renaturation of a novel human single-chain Fv antibody against SARS-CoV. |
Page:692—697 |
| 58. Construction and expression of single chain variable fragments (ScFv) against human CD19 antigen. |
Page:686—691 |
| 59. Enhancement of immune responses to hepatitis B DNA vaccine by superantigen SEA in mice. |
Page:681—685 |
| 60. Chloroplast genetic engineering: a new approach in plant biotechnology. |
Page:674—680 |
| 61. Research on gene expression data based on clustering/classification technology. |
Page:667—673 |
| 62. Optimization of fermentation of recombinant human Endostatin (rh-Endostatin) expression in Escherichia coli. |
Page:662—666 |
| 63. A model for amino acid composition and optimum pH in G/11 xylanase based on neural networks. |
Page:658—661 |
| 64. Studies on the catalytic performance of lignin peroxidase in nonionic reversed micelles. |
Page:654—657 |
| 65. Effects of seeding methods on seeding efficiency and initial cell distribution in 3-D scaffolds. |
Page:649—653 |
| 66. Glyoxylate cycle is required for the overproduction of glutamate but is not essential for Corynebacterium glutamicum growth on glucose. |
Page:270—274 |
| 67. Improved biochemical characteristics of human disulfide-stabilized Fv fragment to rabies virus. |
Page:265—269 |
| 68. Expression of GP5-M fusion protein of porcine reproductive and respiratory syndrone virus (PRRSV) and establishment of ELISA diagnose based on the recombinant fusion protein. |
Page:259—264 |
| 69. Purification and activity assay of HSA-AX15 (R13K) fusion protein expressed in Pichia pastoris. |
Page:254—258 |
| 70. Purification and characteristics of creatininase from Arthrobacter sp. |
Page:250—253 |
| 71. Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber). |
Page:244—249 |
| 72. Several indirect methods for analysis of CD4 self-association and its function in stable CD4-MHC-II binding. |
Page:239—243 |
| 73. Effects of physical and chemical factors on hairy root growth and flavonoids biosynthesis in the cultures of Saussurea medusa Maxim hairy root. |
Page:233—238 |
| 74. Micro machining of micro-cantilever probes for efficient deposition for biochips. |
Page:227—232 |
| 75. Study on expression of genes in Tamarix androssowii under NaHCO3 stress using gene chip technology. |
Page:220—226 |
| 76. Expression and bioactive characterization of bacteriophage lysin gene of Bacillus anthracis in Escherichia coli. |
Page:216—219 |
| 77. Development of monoclonal antibodies against SARS-CoV and identification of antigenic epitopes. |
Page:211—215 |
| 78. Cloning of CTB-PROIN fusion gene and its expression in Escherichia coli. |
Page:204—210 |
| 79. Over-expression in Escherichia coli and characterization of apolipoprotein AI. |
Page:198—203 |
| 80. Gene knockout and knockin on the Escherichia coli lac operon loci using pBR322-red system. |
Page:192—197 |
| 81. Expression patterns of PPARgamma2 in differentiation of mouse embryonic stem (ES) cells into adipocytes. |
Page:187—191 |
| 82. Expression of target gene in eukaryotic cells driven by prokaryotic T7 promoter and its RNA polymerase. |
Page:182—186 |
| 83. Advances on CO2 fixation by microalgae. |
Page:177—181 |
| 84. Advances in the research of differentiation of embryonic stem cells into hepatocytes. |
Page:171—176 |
| 85. Construction and characterization of TetR and GFP fusion protein. |
Page:97—101 |
| 86. Solid-phase synthesis and biological characterization of S12A-HNTX-IV and R29A-HNTX-IV: two mutants of hainantoxin-IV. |
Page:92—96 |
| 87. The structure-function relationship of thermostable beta-glycosidase from the thermophilic eubacterium Thermus nonproteolyticus HG102. |
Page:84—91 |
| 88. Expression of acidophilic alpha-amylase from Alicyclobacillus acidocaldarius. |
Page:78—83 |
| 89. Purification and characterization of a monofunctional catalase from an alkaliphilic Bacillus sp. F26. |
Page:71—77 |
| 90. Enhancement of hypericin production and cell growth of Hypericum perforatum L. suspension cultures by nitric oxide. |
Page:66—70 |
| 91. Establishment of embryogenic cell suspension culture and plant regeneration of edible banana Musa acuminata cv. Mas (AA). |
Page:58—65 |
| 92. Hydrophobicity of reactive site loop of SCCA1 affects its binding to HBV. |
Page:52—57 |
| 93. Characterization of murine leukemia virus recombinants that express H5N1 subtype avian influenza virus hemagglutinin glycoproteins. |
Page:47—51 |
| 94. Development of a fusion toxin IL15M-PEdelta293 based on a receptor-specific IL-15 antagonist. |
Page:42—46 |
| 95. Transfection and expression of hRI gene on human umbilical blood stem cells and gene therapy for mouse melanoma. |
Page:36—41 |
| 96. Screening of TACE peptide inhibitors from a phage display random 15-peptide library by recombinant TACE ecotodomain. |
Page:30—35 |
| 97. Cloning of mMR-1 gene and expression in Pichia pastoris systems. |
Page:25—29 |
| 98. Specific identification of (R)-3-hydroxyacyl-ACP: CoA transacylase gene from Pseudomonas and Burkholderia strains by polymerase chain reaction. |
Page:19—24 |
| 99. NF-kappaB tumorigenesis and drug developement. |
Page:12—18 |
| 100. Recent advances in structures and relative enzyme properties of xylanase. |
Page:6—11 |
| 101. Metabonomics and its applications. |
Page:1—5 |
| 102. Construction of flocculation selective vector and expression of beta-glucosidase gene in Saccharomyces cerevisiae. |
Page:167—170 |
| 103. High expression of the foot-and-mouth disease's structural protein P1 in Escherichia coli and analysis of its biology activity. |
Page:163—166 |
| 104. Generation of transgenic mice for hygromycin and neomycin resistance genes and studies on transgene expression. |
Page:159—162 |
| 105. GFP reporter gene under the direction of chicken ovalbumin gene promoter expressed in the CHO cell and in the primary cell cultures of chicken oviduct. |
Page:154—158 |
| 106. Study on cloning of sisomicin-resistant gene (sisR) from Micromonospora inyoensis. |
Page:149—153 |
| 107. Isolation of the capsid protein gene of maize dwarf mosaic virus and its transformation in maize. |
Page:144—148 |
| 108. Cloning of ACA gene promoter and preliminary study of its function. |
Page:139—143 |
| 109. Expression of capsid gene of Chinese isolate of rabbit hemorrhagic disease virus in Pichia pastoris. |
Page:135—138 |
| 110. Purification of ovalbumin from hen egg white by high-speed counter-current aqueous two-phase chromatography. |
Page:129—134 |
| 111. Effect of flocculence of a self-flocculating yeast on its tolerance to ethanol and the mechanism. |
Page:123—128 |
| 112. Differential effect of temperature on Plt and PCA synthesis in a rsmA inactivated mutant strain of Pseudomonas sp. M-18. |
Page:118—122 |
| 113. Continuous ethanol fermentation using self-flocculating yeast strain and bioreactor system composed of multi-stage tanks in series. |
Page:113—117 |
| 114. The effect of fermentation conditions on glycosylation of recombinant human interferon omega in yeast Pichia pastoris. |
Page:107—112 |
| 115. Neural network detection of abnormalities in fed-batch fermentation. |
Page:102—106 |
| 116. Cloning of a rice gene encoding a putative BPH-inducible GST-like protein. |
Page:646—648 |
| 117. Cloning and analysis of psaB cDNA of Dunaliella salina. |
Page:642—645 |
| 118. Expression of SARS spike gene in Shizomycete pombe. |
Page:638—641 |
| 119. Immobilization of cells by macro-porous NaCS-PDMDAAC capsules and cultivation in shaking flask and bubble bioreactor. |
Page:633—637 |
| 120. Continuous ethanol fermentation using self-flocculating yeast in multi-stage suspended bioreactors coupled with directly recycling of waste distillage. |
Page:628—632 |
| 121. Perfusion culture of hematopoietic cells in a stirred tank bioreactor. |
Page:622—627 |
| 122. Optimization of cultural condition of genetic engineering strain for antibiotic peptide adenoregulin and research on its fed-batch cultivation. |
Page:615—621 |
| 123. Media optimization for the novel antimicrobial peptide by Bacillus sp. fmbJ224. |
Page:609—614 |
| 124. Effects of dilution rates on the oscillatory behaviors of a very high gravity continuous ethanol fermentation system. |
Page:604—608 |
| 125. On-column refolding and purification of human EGF receptor L2 domain inclusion body overexpressed in Escherichia coli. |
Page:597—603 |
| 126. Soluble expression and characterization of disulfide bond-rich subdomains of membrane protein p185 in Escherichia coli. |
Page:590—596 |
| 127. Modeling for prediction of arabinoxylans solubilization and endo-xylanase random attacking during mashing. |
Page:584—589 |
| 128. Fabrication of scaffold with controlled porous structure and flow perfusion culture in vitro. |
Page:579—583 |
| 129. The construction and characterization of human pro-urokinase mutant. |
Page:573—578 |
| 130. Overexpression of a sweet protein monellin in Escherichia coli. |
Page:568—572 |
| 131. High-level expression of the potential vaccine antigen TSO18 of Taenia solium in Pichia pastoris. |
Page:563—567 |
| 132. Molecular cloning and expression of anti-tumor adhesion peptide (beta3). |
Page:558—562 |
| 133. Construction and expression of a fusion protein made of tissue-type plasminogen activator and hirudin in Pichia pastoris. |
Page:553—557 |
| 134. Inducing synthesis of LacA from Trametes sp. AH28-2 and cloning & analysis of 5'-terminal sequence of transcription control of the gene. |
Page:547—552 |
| 135. Expression of the recombinant SARS coronavirus nucleocapsid protein in Pichia pastoris and identification of its bioactivity. |
Page:540—546 |
| 136. Plasmid construction, expression, immunogenicity and protective efficacy of recombinant protein candidate vaccine of respiratory syncytial virus. |
Page:534—539 |
| 137. Construction of a baculovirus transfer vector and expression of baculovirus-mediated gfp gene in larvae of Spodoptera litura. |
Page:530—533 |
| 138. Synthesis of medium-chain-length polyhydroxyalkanoate (mcl PHA) in type I PHA synthase negative mutant of Aeromonas hydrophila. |
Page:524—529 |
| 139. Construction of recombinant plasmid using Neo/E Technology. |
Page:520—523 |
| 140. RNA interference and its application in inhibiting HIV-1 infection. |
Page:516—519 |
| 141. Nonexpressor of pathogenesis-related genes 1 (NPR1): a key node of plant disease resistance signalling network. |
Page:511—515 |
| 142. Progress of EBNA1/oriP-based plasmid applied in gene therapy. |
Page:507—510 |
| 143. Red/ET recombination and its biomedical applications. |
Page:502—506 |
| 144. Progress in single-domain antibody derived from heavy-chain antibody. |
Page:497—501 |
| 145. Production of functional lipids by lipase-catalyzed acidolysis of lard in solvent free system. |
Page:493—496 |
| 146. The study of transformation of tobacco with the stress responsible gene BoRS1 from Brassica oleracea var. acephala. |
Page:489—492 |
| 147. Construction of mutant population of differential race of Xa23 resistant to rice bacterial blight and avirulence activity identification of mutants. |
Page:486—488 |
| 148. Effect of calcium on aggregation and growth of 293 cells. |
Page:482—485 |
| 149. Cloning and expression of human manganese superoxide dismutase cDNA in Pichia pastoris. |
Page:478—481 |
| 150. Expression and detection of recombinant directing toxin SL120 in Pichia pastoris. |
Page:473—477 |
| 151. Simultaneous purification of superoxide dismutase, catalase and hemoglobin from bovine erythrocyte lysate with PEG 600 as chaperon. |
Page:466—472 |
| 152. Extraction and purification of the Klebsiella pneumoniae capsular polysaccharide and the effection on the cell immunoactivity. |
Page:461—465 |
| 153. Preparation and activity analysis of RGD-mSAK (K130T, K135R). |
Page:456—460 |
| 154. Separation of correctly refolded and mis-refolded consensus interferon by hydrophobic interaction chromatography. |
Page:451—455 |
| 155. Purification and monosaccharide composition of saponin from Asparagus officianlis L. |
Page:446—450 |
| 156. The periodic change of environment factors in solid state fermentation and effect on microorganism fermentation. |
Page:440—445 |
| 157. Comparison of the proteome of the sporutated oocysts of Eimeria tenella diclazuril sensitive strain with diclazuril resistant strain. |
Page:435—439 |
| 158. Studies on fermentation conditions and purification of mutant human interleukin-2 expressed in Pichia pastoris. |
Page:430—434 |
| 159. Synthesis of diacylglycerol using immoblized regiospecific lipase in continuously operated fixed bed reactors. |
Page:425—429 |
| 160. Purification and characterization of a lysozyme from a marine microorganism. |
Page:420—424 |
| 161. Hydrophobic interaction between beta-sheet B1 and B2 in xylanase XYNB influencing the enzyme thermostability. |
Page:414—419 |
| 162. Identification and mode of action of a xylanase A purified from the culture filtrate of Bacillus pumilus WL-11. |
Page:407—413 |
| 163. Effects of methyl jasmonate and salicylic acid on phenylethanoid glycosides synthesis in suspension cultures of Cistanche deserticola. |
Page:402—406 |
| 164. Flk1+ mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice. |
Page:396—401 |
| 165. Cloning and expression of Fusarium moniliforme CGMCC 0536 D-lactonohydrolase gene in Escherichia coli. |
Page:390—395 |
| 166. Co-expression of gpd1 and hor2 from Saccharomyces cerevisiae in Escherichia coli. |
Page:385—389 |
| 167. Expression of the soluble human Fas ligand in Dictyostelium discoideum. |
Page:380—384 |
| 168. Sequencing and expression analysis of a novel HMW-glutenin gene Kx from Crithopsis delileana. |
Page:375—379 |
| 169. Recombination and expression of ORF1 and ORF2 gene of porcine circovirus type 2 and gene of pseudorabies virus. |
Page:370—374 |
| 170. Subcellular localization and tissues expression profile of hGPCRc: an orphan G protein-coupled receptor. |
Page:365—369 |
| 171. The construction of recombinant AAV vector expressing HSVtk gene controlled by Tet-On and the detection of its activity. |
Page:360—364 |
| 172. Soluble expression of recombinant human apoliprotein A-I-Milano in Escherichia coli. |
Page:354—359 |
| 173. Recent advances on the structure and catalytic mechanism of hydrogenase. |
Page:348—353 |
| 174. Artificial transcription factors as tools for gene expression manipulation. |
Page:341—347 |
| 175. Establishment of immunomagnetic capture-fluorescent PCR detection method for Campylobacter jejuni. |
Page:336—340 |
| 176. Utilizing tabacco genomic DNA to construct nearly random peptide libraries. |
Page:332—335 |
| 177. Influence of nitrogen source NH4 Cl concentration on curdlan production in Alcaligenes faecalis. |
Page:328—331 |
| 178. Characteristics of a new fibrinolytic enzyme produced from Rhizopus chinensis 12#. |
Page:323—327 |
| 179. Construction and primary analysis of subtractive library induced by soybean mosaic virus (SMV). |
Page:320—322 |
| 180. Expression of hemagglutinin of avian influenza virus (AIV) and its application in diagnosis of AIV H9 subtype. |
Page:315—319 |
| 181. Construction, expression and purification of repeat fragment polymer of oInhibin alpha-subunit N terminal 1-33 fragment. |
Page:311—314 |
| 182. Normal mice derived from oocytes following intracytoplasmic sperm injection (ICSI). |
Page:305—310 |
| 183. Cloning, expression, purification of protein kinase Cdelta and its preliminary application in drug lead compounds screening. |
Page:300—304 |
| 184. Cloning and expression of the Apx IVA gene of Actionbacillus pleuroneumoniae and development of an indirect ApxIVA-ELISA. |
Page:294—299 |
| 185. Expression, identification and bioactivity characterization of an anti-CD3/anti-CD20 bispecific single-chain antibody. |
Page:289—293 |
| 186. Separation and purification of PEGylated rhG-CSF by two-step ion-exchange chromatography. |
Page:284—288 |
| 187. Operation performance of membrane nitrification bioreactor for ammonia-containing wastewater. |
Page:279—283 |
| 188. LC-ESI/MS identification and reduction of impurities in spiramycin fermentation. |
Page:275—278 |
