| 1. Production of mammary gland bioreactor by gene targeting of somatic cells. |
Page:767—770 |
| 2. Chemical induction -- new strategy of oocyte enucleation. |
Page:763—766 |
| 3. Expression of recombinant human zona pellucida-3 protein (rhZP3) in Pichia pastoris. |
Page:758—762 |
| 4. Flow model of internal-loop granular sludge bed nitrifying reactor. |
Page:754—757 |
| 5. The construction and expression of superantigen SEA and antimelanoma ScFv fusion gene. |
Page:750—753 |
| 6. CaCO3 stimulates alpha-ketoglutarate accumulation during pyruvate fermentation by Torulopsis glabrata. |
Page:745—749 |
| 7. Purification and fingerprinting development of Salvia miltiorrhiza Bunge by high-speed counter-current chromatography. |
Page:740—744 |
| 8. Effects of dissolved oxygen and pH on Candida utilis batch fermentation of glutathione. |
Page:734—739 |
| 9. Screening and characterization of DNA aptamers with hTNF-alpha binding and neutralizing activity. |
Page:730—733 |
| 10. Purification and characterization of glutamate dehydrogenase. from Corynebacterium glutamicum S9114. |
Page:725—729 |
| 11. The breeding and culture condition optimization of a high-biomass, selenium-enriched yeast strain. |
Page:720—724 |
| 12. Enhancements in ethanol tolerance of a self-flocculating yeast by calcium ion through decrease in plasmalemma permeability. |
Page:715—719 |
| 13. Fermentative production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by recombinant Aeromonas hydrophila 4AK4 (pTG01). |
Page:709—714 |
| 14. Modification of the antiapoptotic ability of H18 hybridoma cells. |
Page:705—708 |
| 15. Study of recombinant stem cell factor. |
Page:698—704 |
| 16. Expression of human apolipoprotein A- I in baculovirus-insect cell system. |
Page:692—697 |
| 17. Influence of the reductase deficient Escherichia coli on the solubility of recombinant proteins produced in it. |
Page:686—691 |
| 18. Selection of a peptide mimic the neutralization epitope of hepatitis E virus with phage peptide display technology. |
Page:680—685 |
| 19. Expression, purification and serological reactivity of a chimeric antigen of GRA6 with P30 from Toxoplasma gondii. |
Page:674—679 |
| 20. Application of Ac/Ds transposon system to genetate marker gene free transgenic plants in rice. |
Page:668—673 |
| 21. Identification and cloning of a novel gene involved in EPS biosynthesis of Xanthomonas campestris pv. campestris. |
Page:661—667 |
| 22. The OPG/RANKL/RANK system and bone resorptive disease. |
Page:655—660 |
| 23. Virulence genes of Agrobacterium tumefaciens and analysis function of its biology. |
Page:651—654 |
| 24. Advances in molecular regulation of artemisinin biosynthesis. |
Page:646—650 |
| 25. Prophylactic and therapeutic vaccines against Alzheimer's disease. |
Page:641—645 |
| 26. Progress in the pathway engineering of ethanol fermentation from xylose utilising recombinant Saccharomyces cerevisiae. |
Page:636—640 |
| 27. Induced activity of nitrate reductase by nitrate and cloning of nitrate reductase gene. |
Page:632—635 |
| 28. Sequence analysis of bacterial transposon in NHX gene of Populus euphratica. |
Page:628—631 |
| 29. Multi-epitope DNA vaccines against avian influenza in chickens. |
Page:623—627 |
| 30. Fermentation behaviors of recombinant Pichia pastoris under inhibited methanol concentration. |
Page:618—622 |
| 31. Two-dimensional gel electrophoresis of matrix metalloproteinases in subretinal fluids. |
Page:613—617 |
| 32. Construction of a SV40 promoter specific artificial transcription factor. |
Page:608—612 |
| 33. Characterization of immobilized aflatoxin-detoxizyme. |
Page:603—607 |
| 34. Expression of Chinese sturgeon cystatin in yeast Pichia pastoris and its proteinase inhibitory activity analysis. |
Page:598—602 |
| 35. On-line monitoring of oxygen uptake rate and its application in hybridoma culture. |
Page:593—597 |
| 36. In vitro suspension and bioreactor culture of hematopoietic cells. |
Page:587—592 |
| 37. Rapid and efficient expression of foreign genes in mammalian cells by baculovirus vectors. |
Page:581—586 |
| 38. Preparation and application of gel chip. |
Page:577—580 |
| 39. Expression and identification of recombinant arresten in Pichia pastoris. |
Page:572—576 |
| 40. Bacillus thuringiensis helper protein P20 affects the formation of Cry1Ab. |
Page:566—571 |
| 41. Cloning of human uracil N-glycosylase and its detection in cancer tissues by quantitative RT-PCR. |
Page:561—565 |
| 42. Expression of recombinant human BMP-6 in Escherichia coli and its purification and bioassay in vitro. |
Page:556—560 |
| 43. Genetically marking of natural biocontrol bacterium Bacillus subtilis strains with green fluorescent protein gene. |
Page:551—555 |
| 44. Construction of a vector conferring herbicide and pest resistance in tobacco plant. |
Page:545—550 |
| 45. Screening of Bacillus thuringiensis strains containing vip3A genes and analysis of gene conservation. |
Page:538—544 |
| 46. Expression of heat-labile enterotoxin and the strategy of purification and storage. |
Page:532—537 |
| 47. Expression of chimeric anti-CD3 IgG antibody in mammalian cells and analysis of its biological activity. |
Page:527—531 |
| 48. Expression plasmid-host strain using chromosome-plasmid balanced lethal system based on the Escherichia coli thyA. |
Page:521—526 |
| 49. The structure and virus-like particle vaccine of the HIV-1 capsid protein. |
Page:516—520 |
| 50. The PKS/NRPS hetero-gene cluster of epothilones. |
Page:511—515 |
| 51. Co-culture of embryos: influencing factors and mechanisms of action. |
Page:502—505 |
| 52. Formation of polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha. |
Page:497—501 |
| 53. Studies on the mechanism of thermostability and thermophilicity change of thermostable alkaline phosphatase and its mutants. |
Page:493—496 |
| 54. Effects of different cooling rates on cryopreservation of hematopoietic stem cells from cord blood. |
Page:489—492 |
| 55. Collagen membrane as scaffold for the three-dimensional cultivation of cardiac cells in vitro. |
Page:484—488 |
| 56. Molecular cloning, expression mutation of myostatin and study on biochemical activity of its C-terminal peptide. |
Page:480—483 |
| 57. CDNA cloning of human leptin and its expression. |
Page:476—479 |
| 58. Application of element and metabolism balancing for the cultivation process with Streptomyces aureofaciens. |
Page:471—475 |
| 59. Feeding of mixed-carbon-resource during the expression phase in cultivation of recombinant Pichia pastoris expressing angiostatin. |
Page:467—470 |
| 60. Establishment and biological properties of hybridoma cell lines secreting anti-IBDV idiotypic antibodies. |
Page:462—466 |
| 61. Recovery of platinum with immobilized Citrobacter freudii XP05 biomass. |
Page:456—461 |
| 62. Expansion of bone marrow LTC-ICs in vitro by mouse fetal liver-derived stromal cell lines. |
Page:450—455 |
| 63. Construction and expression of anti-CD3/ anti-Pgp Diabody. |
Page:444—449 |
| 64. Site-directed mutagensis of the major antigen E2 gene of CSFV, its high level expression in Escherichia coli and the immunonicity of recombinant E2 protein. |
Page:439—443 |
| 65. Construction of the attenuated Salmonella typhimurium strain expressing Helicobacter pylori conservative region of adhesin antigen. |
Page:433—438 |
| 66. The effects of the Smad3-knockout on the hematopoiesis of mouse. |
Page:428—432 |
| 67. Expression of phosphoprotein P2 of Cysticercus cellulosae in Pichia pastoris and its application. |
Page:424—427 |
| 68. Optimization of T-dNA insertional mutagenesis and analysis of mutants of Magnaporthe grisea. |
Page:419—423 |
| 69. Establishment of Spodoptera exigua multicapsid nucleopolyhedrovirus BAC-TO-BAC expression system. |
Page:412—418 |
| 70. Establishment of gene transduction system in geldanamycin producer - Streptomyces hygroscopicus 17997 and its application for gene disruption experiment. |
Page:407—411 |
| 71. Gene cloning, soluble expression and activity analysis of rSEA. |
Page:402—406 |
| 72. Expression of gene aiiA carrying the promoter of gene cry3Aa in Bacillus thuringiensis. |
Page:397—401 |
| 73. Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus. |
Page:392—396 |
| 74. Advances in apolipoprotein A- I and it's anti-atherosclerosis properties. |
Page:387—391 |
| 75. Using single primer PCR to amplify the orf25 gene fragment related to plant male-sterility in Salicornia europaea. |
Page:120—123 |
| 76. The preparation of fructose-modified chitosan microcarrier and culture of primary rat hepatocyte. |
Page:116—119 |
| 77. Expression of phytase gene in Bombyx mori. |
Page:112—115 |
| 78. Construction of recombinant adenoviral vector overexpressing human HIF-1alpha gene. |
Page:107—111 |
| 79. High expression and characterization of human parathyroid hormone in Escherichia coli. |
Page:102—106 |
| 80. Studies of enzymatic synthesis of biodiesel. |
Page:97—101 |
| 81. 2-dimensional gel electrophoresis on protein patterns from suspension cultures of Taxus cuspidate induced by SA. |
Page:92—96 |
| 82. Fed-batch fermentation of Escherichia coli that express fab fragment of anti-HBsAg. |
Page:87—91 |
| 83. Quantitative studies of the production phase in rHSA fermentation. |
Page:81—86 |
| 84. Biotransformation of benzene to cis-1,2-dihydroxycyclohexa-3,5-diene using recombinant Escherichia coli JM109 (pKST11). |
Page:74—80 |
| 85. Study of the expression membrane protein LASS2. |
Page:69—73 |
| 86. Fusion expression, purification and bioactivity assay of CpTI in Escherichia coli. |
Page:63—68 |
| 87. Orange fluorescent protein--modification of green fluorescent protein GFPxm. |
Page:56—62 |
| 88. Secreted expression of Bacillus pumilus xylanase gene in Pichia pastoris and study on enzymatic properties. |
Page:50—55 |
| 89. Induction and culture of hairy boots in Phytolacca esculenta and its saponin production. |
Page:46—49 |
| 90. Expression of xylanase gene xynA from Streptomyces olivaceoviridis A1 in Escherichia coli and Pichia pastoris. |
Page:41—45 |
| 91. Construction and biological activity study of human osteoprotegerin expressing adenoviral system. |
Page:35—40 |
| 92. Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos. |
Page:30—34 |
| 93. Safety, stability and immunogenicity of an oral DNA vaccine against Newcastle disease. |
Page:24—29 |
| 94. Expression of a human single-chain Fv antibody against HBsAg in Pichia pastoris. |
Page:19—23 |
| 95. Study on relationship between length of homologous sequences and chromosomic recombination rate in Saccharopolyspora erythraea. |
Page:13—18 |
| 96. Epigenetic modifications and its impact on animal cloning. |
Page:9—12 |
| 97. Oxidative refolding of proteins. |
Page:1—8 |
| 98. Expression of recombinant plasmid pcDNA3.1/P12X3C with multi-genes of foot-and-mouth disease virus in BHK-21 cells. |
Page:376—379 |
| 99. Study on the culture of crown gall from Panax quinquefolium and the prouction of its secondary metabolites--Ginsenosides Re and Rg1. |
Page:372—375 |
| 100. Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim. |
Page:368—371 |
| 101. Protoplast isolation and transformation of Exserohilum turcicum. |
Page:364—367 |
| 102. Kinetic models for the effect of temperature on batch glutathione fermentation by Candida utilis. |
Page:358—363 |
| 103. Molecular cloning and sequence analysis of ussurin, a new metalloproteinases/disintegrin from Gloydius ussuriensis. |
Page:353—357 |
| 104. Establishment and application of the model of islet impaired by NO free radical released from streptozotocin. |
Page:349—352 |
| 105. Uptake of nickel from industrial wastewater by genetically engineered Escherichia coli JM109. |
Page:343—348 |
| 106. Soluble expression of peptide containing MUC1/Y-specific epitope in Escherichia coli and preparation of the antibody. |
Page:337—342 |
| 107. The preparation and application of cross-linked urease aggregates. |
Page:332—336 |
| 108. Purification and properties of Bacillus subtilis SA-22 endo-1, 4-beta-D-mannanase. |
Page:327—330 |
| 109. Construction of an anti-apoptosis CHO cell line for biopharmaceutical production. |
Page:322—326 |
| 110. The role of cortical microtubules in moss protonemal cells during dehydration/rehydration cycle. |
Page:317—320 |
| 111. The investigation of hematopoietic capacity of HPP-CFC derived from murine embryonic stem cells in vitro and in vivo. |
Page:312—316 |
| 112. Induction of hairy roots of Pueraria phaseoloides and its culture in liquid and solid medium. |
Page:307—311 |
| 113. Co-expressions of phosphoenolpyruvate synthetase A (ppsA) and transketolase A (tktA) genes of Escherichia coli. |
Page:301—306 |
| 114. HRCA and application in detection of genetically modified plant. |
Page:294—300 |
| 115. Preparation of antibodies against a novel leucine-zipper protein, Ecp. |
Page:291—293 |
| 116. Secretion expression of Mt. heat shock protein 70 in Pichia pastoris and identification of the protein. |
Page:286—290 |
| 117. Construction of gene library of Arthrobacter BT801 and isolation & expression of hydantoinase gene. |
Page:281—285 |
| 118. Construction of MAGE-3 prokaryotic expression plasmid and its expression in Escherichia coli. |
Page:277—280 |
| 119. One amino acid mutation in an anti-CD20 antibody fragment that affects the yield bacterial secretion and the affinity. |
Page:272—276 |
| 120. Preliminary study of ALK3 downstream genes related to ventricular septum defect. |
Page:267—271 |
| 121. Advances in the research of human defensins. |
Page:261—266 |
| 122. Protein splicing and its application. |
Page:249—254 |
| 123. Advances in analysis techniques of phosphoproteome. |
Page:244—248 |
| 124. The action of S1 nuclease and a cloning strategy for microcircular DNAs. |
Page:240—243 |
| 125. Studies on immobilized cellobiase. |
Page:236—239 |
| 126. Overexpression of Penicillium expansum lipase gene in Pichia pastoris. |
Page:231—235 |
| 127. Primary targeting of functional regions involved in transcriptional regulation on watermelon fruit-specific promoter WSP. |
Page:227—230 |
| 128. Fermentation and purification of recombinant alpha-galactosidase from Pichia pastoris. |
Page:223—226 |
| 129. Preparation and functional study of an adenovirus vector expressing human plasminogen kringle 5 gene. |
Page:217—222 |
| 130. Effects of nutrient conditions and fed-batch culture on CoQ10 production by Rhizobium radiobacter WSH2601. |
Page:212—216 |
| 131. Saccharomyces cerevisiae B5 efficiently and stereoselectively reduces 2'-chloroacetophenone to R-2'-chloro-1-phenylethanol in the presence of 5% ethanol. |
Page:206—211 |
| 132. Quantitation & optimization of guanosine fermentation process: prevention of NH4+ accumulation increases guanosine production by 70%. |
Page:200—205 |
| 133. Biosynthesis of poly (3-mercaptopropionate) and poly (3-mercaptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli. |
Page:195—199 |
| 134. Screening of new binding partners of CIKS with yeast two-hybrid system. |
Page:190—194 |
| 135. Site-directed mutagenesis of NisinZ and properties of NisinZ mutants. |
Page:185—189 |
| 136. Expression of Mortierella isabellina delta6-fatty acid desaturase gene in gamma-linolenic acid production in transgenic tobacco. |
Page:178—184 |
| 137. Cloning and expression of L-N-carbamoylase gene from Arthrobacter BT801 in Escherichia coli. |
Page:174—177 |
| 138. Establishment of stably expressed human RANTES gene in prunella vulgaris cell clone. |
Page:168—173 |
| 139. Expression of a DNA fragment encoding the active domain of human TNF related apoptosis inducing ligand in pichia pastoris. |
Page:163—167 |
| 140. Enhancement of cellular immune response to DNA vaccine encoding hepatitis C virus core and envelope 2 fusion antigen by murine Fms-like tyrosine kinase 3 ligand. |
Page:158—162 |
| 141. Accelerated improvement of bacterial blight resistance of 'Shuhui527' using molecular marker-assisted selection. |
Page:153—157 |
| 142. Identification and expression analysis of a full-length cDNA encoding a Kandelia candel tonoplast intrinsic protein. |
Page:147—152 |
| 143. The potential of avian cytokines as immunotherapeutics and vaccine adjuvants. |
Page:141—146 |
| 144. Advances of studies on mesenchymal stem cells. |
Page:136—140 |
| 145. Vascular-specific promoters and cis-regulatory elements. |
Page:131—135 |
