| 1. Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris. |
Page:348—351 |
| 2. In vitro culture of umbilical cord blood MNC and CD34+ selected cells. |
Page:343—347 |
| 3. Site-directed mutation of PoIFN-alpha and its expression in Escherichia coli. |
Page:339—342 |
| 4. A novel resolution vector with Bacillus thuringiensis plasmid replicon ori44. |
Page:335—338 |
| 5. Study on construct and expression of synthetic genes encoding spider dragline silk in Escherichia coli. |
Page:331—334 |
| 6. Construction and in vitro activity of specific dual-ribozyme against alpha 1 (I) and (III) procollagen genes. |
Page:327—330 |
| 7. Callus induction and regeneration from mature seeds of indica rice minghui 63 and anti-fungal assay of transgenic rice plants. |
Page:323—326 |
| 8. Study on optimization of expression, purification, properties and biological function of recombinant human sBLyS. |
Page:318—322 |
| 9. Screening for resistance gene candidate from a genomic TAC library of Triticum aestivum-Haynaldia villosa translocation line 6VS/6AL by pooled PCR. |
Page:313—317 |
| 10. Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker. |
Page:308—312 |
| 11. Overexpression and detection of the mutated glucose isomerase GIG138P and GIG138P-G247D in Streptomyces lividans. |
Page:304—307 |
| 12. The expression of Aeromonas hydrophila ompTS gene and the immunogenicity of recombinant OmpTS. |
Page:300—303 |
| 13. Production of SAM by recombinant Pichia pastoris. |
Page:295—299 |
| 14. Refolding and purification of the huGM-CSF(9-127)-IL-6(29-184) fusion protein. |
Page:291—294 |
| 15. A transgenic mouse that targets the expression of Cre recombinase in pancreatic tissue. |
Page:286—290 |
| 16. The development of peptide nucleic acid in gene regulation. |
Page:282—285 |
| 17. Gene and gene engineering of carotenoid biosynthesis. |
Page:276—281 |
| 18. Progresses in mouse chromosome engineering. |
Page:272—275 |
| 19. Development of the genetic transformation system in extremely halophilic archaea. |
Page:267—271 |
| 20. Escherichia coli disulfide-forming related proteins: structures, functions and their application in gene engineering for expressing heterologous proteins in Escherichia coli. |
Page:261—266 |
| 21. The electron microscopic in situ hybridization and its application. |
Page:249—252 |
| 22. Protein microarrays and their medical applications. |
Page:246—248 |
| 23. Lipase-catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester in organic solvent. |
Page:242—245 |
| 24. Study of alginate/chitosan microcapsules for immobilization of Escherichia coli DH5 alpha. |
Page:239—241 |
| 25. Study on the kinetics of immobilized cells of Brevibacterium ammoniagenes MA-2 and Brevibacterium flavum MA-3. |
Page:235—238 |
| 26. Protective effect induced by vaccination with a partial cDNA expression library of Schistosoma japonicum in mice. |
Page:231—234 |
| 27. Study on production of acrylamide by microbial method (II)--enzyme catalytic kinetics and de-active dynamics of nitrile hydratase. |
Page:225—230 |
| 28. Study of immobilization of Aspergillus oryzae mycelium aided by artificial neural network. |
Page:221—224 |
| 29. Construction of recombinant strain expressing enterotoxigenic Escherichia coli K88ac-ST1-LTB fusion protein. |
Page:216—220 |
| 30. Cloning and expression of spinach glycolate oxidase in Escherichia coli. |
Page:212—215 |
| 31. Study on the expression of E2 gene of classical swine fever virus in Pichia pastoris and the immunological activity of its expression product. |
Page:208—211 |
| 32. Induced-effects by additional taxol in suspension cultures of Taxus cuspidata cells. |
Page:204—207 |
| 33. Construction of Saccharopolyspora erythraea M synthesizing a novel ketolide 3-deoxy-3-oxo-erythronolide B. |
Page:198—203 |
| 34. Expression of adenovirus-mediated pGH cDNA with first intron in CHO cells. |
Page:193—197 |
| 35. Expression and characterization of envelope protein 2 gene of hepatitis G virus in Pichia pastoris. |
Page:187—192 |
| 36. Cloning of Xanthomonas campestris pv. campestris pathogenicity-related gene sequences by TAIL-PCR. |
Page:182—186 |
| 37. Cloning, expression and bio-activity assay of chimeric fusion protein sTNFRII-IgG Fc. |
Page:178—181 |
| 38. Expression and purification of recombinant huwentoxin-I in Pichia pastoris. |
Page:172—177 |
| 39. Construction of the mutants of rice nonspecific lipid transfer protein and expression comparison in two kinds of thioredoxin fusion expression vectors. |
Page:167—171 |
| 40. Tagging and mapping of QTLs controlling lint yield and yield components in upland cotton (Gossypium hirsutum L.) using SSR and RAPD markers. |
Page:162—166 |
| 41. Cloning, sequencing and high expression in Escherichia coli of D-hydantoinase gene from Burkholderia pickettii. |
Page:149—154 |
| 42. Transgenic animals bioreactors. |
Page:144—148 |
| 43. The mechanism and application of posttranscriptional gene silencing. |
Page:140—143 |
| 44. The progress of expressing vector for mammary gland bioreactor. |
Page:136—139 |
| 45. Current state of establishing and maintaining human embryonic stem cell lines and key problems in these studies. |
Page:131—135 |
| 46. Visualization of the interaction between IL-6 and IL-6R by imaging ellipsometry. |
Page:99—101 |
| 47. Studies on lipase-catalyzed synthesis of ethylglucoside lactate in non-aqueous phase. |
Page:94—98 |
| 48. Continuous biosynthesis of epoxypropane in a methanotrophic attached-films reactor. |
Page:89—93 |
| 49. Enzymatic synthesis of monoglycerides in microaqueous media by using lipase from Pseudomonus fluorescens. |
Page:84—88 |
| 50. Cloned goats produced from the somatic cells of an adult transgenic goat. |
Page:79—83 |
| 51. Studies on the optimal expression condition, purification and its characterization of ScFv-2F3. |
Page:74—78 |
| 52. Hairy-root culture of Polygonium multiflorum Thunb. and the production of its active constituents-anthraquinones. |
Page:69—73 |
| 53. Cloning and expression of a thermostable beta-glycosidase gene from Thermus nonproteolyticus HG102. |
Page:63—68 |
| 54. Effect of trypsin on the rat keratinocyte separation and subculture. |
Page:59—62 |
| 55. Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase. |
Page:55—58 |
| 56. Extraction of the salt-tolerant cDNA in mangrove Avicennia marina by mRNA differential display. |
Page:51—54 |
| 57. Avirulence genes of plant pathogens. |
Page:5—9 |
| 58. Nucleotide sequence and protein sequence analysis of GL-7-ACA acylase from Pseudomonas sp. 130. |
Page:45—50 |
| 59. Expression of the human soluble low density lipoprotein receptor in methylotropic yeast. |
Page:40—44 |
| 60. Cloning and expression of extracellular domain of prostate specific membrane antigen in Escherichia coli and preparation of polyclonal antibody. |
Page:35—39 |
| 61. Expression of human interleukin-11 in cell culture and larvae of silkworm. |
Page:30—34 |
| 62. The construction of the hammerhead ribozyme genes targeting against apple scar skid viroid and its activity detection in vitro. |
Page:25—29 |
| 63. Couple production of human calcitonin and rat peptidylglycine alpha-amidation monooxygenase in insect cells. |
Page:20—24 |
| 64. Na+/H+ antiporter and plant salt tolerance. |
Page:16—19 |
| 65. The thinking about modern biological technology. |
Page:121—123 |
| 66. Strategies of functional analysis of new genes. |
Page:117—120 |
| 67. Cloning of 5', 3' flanking sequence of ovine BLG and regulating the expression of GFP in mammary gland cell line. |
Page:112—116 |
| 68. Attachment and growth of cultured fibroblast cells on chitosan/PHEA-blended hydrogels. |
Page:109—111 |
| 69. Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli. |
Page:106—108 |
| 70. Genome analysis of transgenic homozygous line "Minghui 63-Xa21". |
Page:102—105 |
| 71. Bioactive compounds from marine sponges and cell culture of marine sponges. |
Page:10—15 |
| 72. Molecular biological progress of hepatocyte growth factor (HGF). |
Page:1—4 |
| 73. Cloning and expression of 21.7 kD protein gene of Schistosoma japonicum (Chinese strain). |
Page:698—702 |
| 74. Progress in purification of human serum albumin. |
Page:761—766 |
| 75. The study on the stable inheritance and expression of foreign gene in transgenic mice. |
Page:758—760 |
| 76. Effects of SCF, LIF and bFGF on mouse spermatogonial stem cells proliferation in vitro. |
Page:754—757 |
| 77. The construction of cDNA expression library from the tentacles of Sagartia rosea. |
Page:749—753 |
| 78. Structure characteristics of ORF2a gene of potato leafroll virus Chinese isolate. |
Page:744—748 |
| 79. Discussion of the methods for establishing embryonic stem cell lines from 129/ter. C57BL/6J mice with high efficiency. |
Page:740—743 |
| 80. Synthesis of partial glycerol caprates by using lipase in nonaqueous media. |
Page:735—739 |
| 81. Study on fermentation condition of alkaline protease gene engineering strain and the purification and characterization of recombinant enzyme. |
Page:729—734 |
| 82. Utilization of sugar cane bagasse hydrolysates for xylitol production by yeast. |
Page:724—728 |
| 83. High density fed-batch culture of Escherichia coli DH5 alpha/pDH-B2m with DO feed-back control of nutrient feeding. |
Page:718—723 |
| 84. The progressive study on gene therapy for hyperphenylalaninemia rats. |
Page:713—717 |
| 85. Biocompatibility of SA/CS-CaCl2/PMCG Microcapsule in cell culture. |
Page:709—712 |
| 86. Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins. |
Page:703—708 |
| 87. The renaturation and purification of RGD-staphylokinase by gel filtration. |
Page:693—697 |
| 88. Cloning and expression product of vip3A gene from Bacillus thuringiensis and analysis of inseceicidal activity. |
Page:687—692 |
| 89. Expression of porcine interferon-gamma gene in Pichia pastoris and its effect of inhibiting porcine reproductive and respiratory syndrome virus. |
Page:683—686 |
| 90. Minimal functional domain of cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuAc) synthetase from Escherichia coli. |
Page:676—682 |
| 91. Comparative analysis of diuretic activities of human ANP gene injected intramuscularly and intravenously in adriamycin-induced nephrotic rats. |
Page:671—675 |
| 92. Construction of recipient strain of expression-secretion system in filamentous fungi. |
Page:667—670 |
| 93. Cloning of the replication origin (oriC) of Streptoverticillum caespitosus and transformation of Streptomyces lividans ZX7. |
Page:662—666 |
| 94. Mapping of BRCT1 domain of BRCA1 with chromatin unfolding activity. |
Page:656—661 |
| 95. Progress in biotechnological production of pyruvic acid. |
Page:651—655 |
| 96. The PTD domain of Tat protein enhance GFP protein delivering into myeloma cell SP2/0. |
Page:644—647 |
| 97. Amplification, cloning and sequence analysis of spider dragline silk cDNA. |
Page:641—643 |
| 98. Induction of petal-like structures from petals of Crocus sativus L. and the differentiation of style-stigma-like structures in vitro. |
Page:638—640 |
| 99. Influence of pH control on the production of curdlan by Alcaligenes faecalis strain. |
Page:634—637 |
| 100. The effect of antioxidants on the in vitro life-span of keratinocyte. |
Page:630—633 |
| 101. Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes. |
Page:626—629 |
| 102. Analysis on metabolic flux shift during guanosine fermentation. |
Page:622—625 |
| 103. Micropatterning of biotin-avidin layers and cell location. |
Page:619—621 |
| 104. Biosynthesis and accumulation of poly(3-hydroxybutyrate) in Vibrio natriegens. |
Page:614—618 |
| 105. Preparation of bovine lipid-free hemoglobin. |
Page:609—613 |
| 106. Cloning of the major antigen region of E2 gene of hog cholera virus and expression in Escherichia coli. |
Page:605—608 |
| 107. Induction of immune responses in mice by hepatitis B virus large envelope DNA vaccine delivered by attenuated Salmonella typhimurium. |
Page:601—604 |
| 108. Cloning of staphylococcal enterotoxin B gene and its highly expression in Escherichia coli. |
Page:597—600 |
| 109. Cloning and expression of kringle 1-3 gene of human plasminogen and the purification and bioactivity of its expressed product. |
Page:593—596 |
| 110. Design and preparation of the multimeric self-cleavable hammerhead ribozyme targeting apple scar skid viroid and its activity detection in vitro. |
Page:588—592 |
| 111. Characterization of inductive synthesis of levoglucosan kinase by a combined strategy of enzymological and fast atom bombardment mass spectrometric analysis. |
Page:583—587 |
| 112. The analysis of Bacillus thuringiensis vegetative insecticical protein gene cloning and expression. |
Page:578—582 |
| 113. BjCHI1 from Brassica juncea displays both chitinase and agglutination activity. |
Page:572—577 |
| 114. Cloning and sequencing of lactase gene from Aspergillus candidus. |
Page:566—571 |
| 115. Purification and characterization of antifungal proteins in triticale seed. |
Page:561—565 |
| 116. Screening of TNF-alpha antagonist peptides from a random peptide library displayed with Escherichia coli flagellar. |
Page:556—560 |
| 117. Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator. |
Page:551—555 |
| 118. The expression of humanized Fab fragment of the anti-HBsAg antibody in methylotropic yeast Pichia pastoris. |
Page:546—550 |
| 119. Fusion expression of human thymosin alpha 1 in Escherichia coli. |
Page:541—545 |
| 120. High expression and identification of DNA mismatch repair gene mutS in Escherichia coli. |
Page:536—540 |
| 121. Progress in the study of the structure and function of Cre recombinase. |
Page:531—535 |
| 122. The methods of identifying differences in mRNA expression. |
Page:521—524 |
| 123. Food-grade gene expression systems for lactic acid bacteria. |
Page:516—520 |
| 124. Differences in gene expression between Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase. |
Page:512—515 |
| 125. The functional expression of humanized ScFv-urokinase fusion protein in Escherichia coli. |
Page:509—511 |
| 126. Enhancing hGH expression level in insect cells by shortening the 5'-UTR of hGH cDNA. |
Page:505—508 |
| 127. Preparation and analysis of oligonucleotide microarray for expression detection of mouse cytokine-associated gene. |
Page:501—504 |
| 128. Expression of cre gene in Escherichia coli and bioassay its expression product. |
Page:497—500 |
| 129. Performance of internal-loop air-lift nitrifying bioreactor. |
Page:492—496 |
| 130. Hydrogen photoproduction from acetate by Rhodopseudomonas palustris. |
Page:486—491 |
| 131. Analysis of salt resistance on the poplar transferred with salt tolerance gene. |
Page:481—485 |
| 132. The changes of rare codons increase expression level of SEA. |
Page:477—480 |
| 133. Expression of polyprotein of infectious bursal disease virus in Bombyx mori. |
Page:472—476 |
| 134. Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal. |
Page:468—471 |
| 135. The study of aggregate of the ORF2 peptide of hepatitis E virus expressed in Escherichia coli. |
Page:463—467 |
| 136. Studies on production, purification and partial characteristics of the extracellular laccase from Armilliria mellea. |
Page:457—462 |
| 137. Analysis of the proteomic changes of the serum of the Smad3 targeted deficient mice. |
Page:452—456 |
| 138. Detection of P53 and K-ras gene mutations in lung cancer with oligonucleotide chip. |
Page:447—451 |
| 139. Anther culture generated stem borer-resistance DH lines of Minghui 81(Oryza sativa L. subsp. indica) expressing modified cry1Ac gene. |
Page:442—446 |
| 140. The construction of shuttle vectors of Brevibacillus brevis-Escherichia coli. |
Page:438—441 |
| 141. Establishment of gene replacement/disruption system through homologous recombination in Amycolatopsis mediterranei U32. |
Page:431—437 |
| 142. Expression of TPO mimetic peptide chimeric proteins with human IgG1 Fc fragments and their biological characters. |
Page:424—430 |
| 143. Construction of a temperature inducible shuttle expression vector and its application in Streptomyces. |
Page:420—423 |
| 144. Modification of Chinese hamster ovary cells. |
Page:415—419 |
| 145. Agroinfiltration, a useful technique in plant molecular biology research. |
Page:411—414 |
| 146. Advances in the application research of bacterial ice nucleation active (ina) genes. |
Page:407—410 |
| 147. Current researches on molecular enzyme engineering. |
Page:401—406 |
| 148. Studies on improving crop nutritional quality through gene engineering. |
Page:381—386 |
| 149. Technological advances of serial analysis of gene expression. |
Page:377—380 |
| 150. Optimization of cultivation conditions in se-enriched Spirulina platensis. |
Page:373—376 |
| 151. Effect of microbial elicitors on sclerotia biomass and carotenoid yield in surface cultures of Penicillium sp.PT95. |
Page:369—372 |
| 152. Cloning and expression of canine interferon-gamma gene in mouse SP2/0 cell line. |
Page:365—368 |
| 153. Continuous perfusion culture hybridoma cells for production of monoclonal antibody. |
Page:360—364 |
| 154. Single-chain urokinase-type plasminogen activator (scu-PA) purification by immuno-affinity chromatography. |
Page:356—359 |
| 155. Cloning and expression of the cecropin B-thanatin hybrid antimicrobial peptide in Escherichia coli. |
Page:352—355 |
| 156. Cholesterol Esterase and Cholesterol Oxidase Immobilized onto Arylamine Glass Beads |
Page:155—161 |
