Korean Journal of Veterinary Research  2015;55(4):241-246

doi:10.14405/kjvr.2015.55.4.241

Establishment of a live vaccine strain against fowl typhoid and paratyphoid.

Sun Hee CHO 1 ; Young Jin AHN ; Tae Eun KIM ; Sun Joong KIM ; Won HUH ; Young Sik MOON ; Byung Hyung LEE ; Jae Hong KIM ; Hyuk Joon KWON

Affiliations

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Keywords

attenuation; fowl typhoid; live vaccine; paratyphoid; rough strain

Country

Republic of Korea

Language

English

Abstract

To develop a live vaccine strain against fowl typhoid and paratyphoid caused by Salmonella serovar Gallinarum biovar Gallinarum (Salmonella Gallinarum) and Salmonella serovar Enteritidis (Salmonella Enteritidis), respectively, several nalidixic acid resistant mutants were selected from lipopolysaccharide (LPS) rough strains of Salmonella Gallinarum that escaped from fatal infection of a LPS-binding lytic bacteriophage. A non-virulent and immunogenic vaccine strain of Salmonella Gallinarum, SR2-N6, was established through in vivo pathogenicity and protection efficacy tests. SR2-N6 was highly protective against Salmonella Gallinarum and Salmonella Enteritidis and safer than Salmonella Gallinarum vaccine strain SG 9R in the condition of protein-energy malnutrition. Thus, SR2-N6 may be a safe and efficacious vaccine strain to prevent both fowl typhoid and paratyphoid.