Purpose:: This study aimed to identify the factors affecting coronavirus disease 2019 (COVID-19) infection control practices of nurses in negative pressure isolation rooms.

Methods:: The participants were 150 nurses working in three hospitals with negative pressure isolation rooms. Data were collected using structured questionnaires and analyzed using an independent t-test, one-way ANOVA, Scheffé test, Pearson correlation coefficients, and multiple regression analysis using the SPSS/WIN 26.0 program.

Results:: Factors significantly influencing the subject's COVID-19 infection control practice include Being married (β=.18, p =.016), working in a ward with negative pressure isolation rooms (β=.44, p <.001), working in an ICU with negative pressure isolation rooms (β=.27, p =.010), COVID-19 infection control attitude (β=.28, p =.001), anxiety for COVID-19 (β=.30, p <.001). The explanatory power of these variables for COVID-19 infection control practice was 24.6% (F=8.67, p <.001).

Conclusion:: It is expected that strategies that utilize positive attitudes which believe that COVID-19 disease may be overcome by COVID-19 infection control practice will help improve emerging infectious diseases infection control practice.