The aim of this study was to investigate histological composition (ratio of anulus fibrosus, cartilage endplate, and nucleus pulposus) of the herniated lumbar intervertebral disc fragments. The relative percentage of anulus fibrosus, cartilage endplate, and nucleus pulposus was examined in the herniated interverterbral disc fragments. The herniated intervertebral discs took out from the 31 patients were stained with H-E. The stained tissues were photographed, and the histological composition of the herniated intervertebral discs was analyzed after marking 3 components on the photograph with microscopic observation. The composition of the herniated lumbar intervertebral disc is 50.7% of nucleus pulposus, 41.3% of anulus fibrosus, and 8.0% of cartilage endplate. The proportion of the endplate in the herniated intervertebral disc in male was tend to be higher than that in female, and the proportion of the nucleus pulposus was tend to be decreased with aging, however, fail to reach statistical significance. This result may provide fundamental information for accessing clinical symptoms including pain in lumbar vertebral disc herniation.