The purpose of this study was to evaluate the effect of different dentin surface treatments on the bond strength of self-adhesive resin cement (G-CEM one). Human permanent molars were sectioned horizontally and standard smear layer was created. Teeth were divided into 4 groups according to the treatment methods: 1) no treatment, 2) 10% polyacrylic acid treatment, 3) exclusive primer (G-CEM one primer) treatment, 4) polyacrylic acid + exclusive primer treatment. After surface treatment, composite resin blocks were cemented with G-CEM one. After storage, specimens were cut into bars to measure the microtensile bond strength. Measured data were statistically analyzed. Confocal laser scanning microscopy was used to observe the bonding interface of cemented surface. Polyacrylic acid surface treatment group showed significantly lower µTBS than other groups (p<0.05). µTBS of exclusive primer treated groups was significantly higher than the control group (p<0.05). As a result, the exclusive primer improved dentin-cement bond strength. Polyacrylic acid treatment alone had negative effect on µTBS of G-CEM one and dentin.