Journal of Public Health and Preventive Medicine 2021;32(2):7-11

doi:10.3969/j.issn.1006-2483.2021.02.002

Analysis of the relationship between Clostridium genus and neonatal necrotizing enterocolitis

Dongyu ZHENG 1 ; Yijing ZHOU 1 ; Yijing ZHOU 1 ; Lubiao YAN 2 ; Shuping HAN 2 ; Hong CHI 3 ; Xuan WANG 4 ; Shiqi ZHEN 1 ; Zhen TANG 1 ; Yun LIU 3 ; Lei LIU 3 ; Han ZHOU 4 ; Xiang HUO 1

Affiliations

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Keywords

Neonatal necrotizing enterocolitis; 16S rRNA metagenomic sequencing; Intestinal Flora; Clostridium

Country

China

Language

Chinese

Abstract

Objective To analyze the correlation between intestinal flora changes and neonatal necrotizing enterocolitis (NEC)through 16S rRNA metagenomic sequencing and bacterial culture. Methods From September 2018 to March 2019, 10 NEC cases and 6 controls were randomly selected in the neonatal ICU ward of Nanjing maternal and child health care hospital to analyze the 16S rRNA metagenomic diversity of the for intestinal flora. The fecal samples and corresponding environmental samples were corrected from 51 cases of NEC children and their case controls to isolate and culture Clostridium. Results The dispersion of samples within the case group was smaller than that of the control group, and the sample diversity was higher than that of the control group. In the isolation and culture of Clostridium, the overall detection rate of Clostridium in the case group was 43.14% (22/51), and the detection rate of Clostridium butyricum was the highest (19.61%, 10/51). There was a statistical difference between the two groups (χ2=5.85, P=0.015 58). All Clostridium strains did not carry the A, B and E type neurotoxin genes. Conclusion: Increased intestinal flora diversity, intestinal flora abundance and changes in the abundance of Clostridium may be closely related to the intestinal environment of children with NEC; Clostridium, especially Clostridium butyricum, may be related to the occurrence of NEC.