Academic Journal of Xi'an Jiaotong University  2010;22(4):260-264

Inhibitory action of docetaxel on the proliferation of HeLa and SiHa cells

Yu-Liang ZOU 1

Affiliations

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Keywords

Cervical cancer; Chemotherapy; Docetaxel; HeLa; Proliferation; SiHa

Country

China

Language

Chinese

Abstract

Objective: To study the inhibitory action of docetaxel (DOC) on the proliferation of HeLa and SiHa cells. Methods: Cell morphological changes were observed with inverted phase contrast microscope. MTT was adopted to test and calculate the cell inhibition ratio. Flow cytometry was used to detect cell cycle. Results: DOC had an obvious concentration-dependent inhibitory effect on the proliferation of both HeLa and SiHa cells. The inhibition ratio of DOC on SiHa was significantly higher than that on HeLa (P<0.05). DOC blocked HeLa at G2/M phase. Under the effect of DOC, the cell cycle of SiHa was not changed much. Conclusion: DOC has an obvious inhibitory action on both HeLa and SiHa cells, which shows a promising prospect of DOC in clinical treatment of cervical cancer.