Journal of Public Health and Preventive Medicine 2020;31(3):22-24

doi:10.3969/j.issn.1006-2483.2020.03.006

Study on the effect of chlorine disinfectants on the destruction of New coronavirus nucleic acid

Maoyi CHEN 1 ; Jie HU 1 ; Chunlin MAO 1 ; Tao SHI 1 ; Ting CHEN 1 ; Yujie ZENG 1 ; Bin LI 1 ; Qiong YI 1 ; Hu LI 1 ; Li WANG 1

Affiliations

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Keywords

Chlorine disinfectants; Inactivation effect; New coronavirus; Fluorescent quantitative PCR

Country

China

Language

Chinese

Abstract

Objective To observe the destructive effects of chlorine disinfectants on the nucleic acid of novel coronavirus, and to assess the feasibility of real-time fluorescent quantitative PCR technology for the evaluation of the disinfection effect of novel coronavirus. Methods A suspension quantitative inactivation test was used to observe the inactivation effects of different concentrations of chlorine containing disinfectants and different action times on the novel coronavirus. RT-PCR method was used to detect novel coronavirus nucleic acid for the disinfection effect evaluation. Results The chlorine disinfectants of 1 000 mg/L and 2 000 mg/L could destroy the nucleic acid of ew coronavirusafter application for over 30s. The chlorine disinfectant of 750 mg/L could destroy the nucleic acid of new coronavirusafter application for over 15min. Chlorine disinfectant of 500 mg/L could not completely destroy the nucleic acid of new coronavirusafter 30 min action. Conclusion The disinfection method stipulated in the national prevention and control plan using 1 000mg / L effective chlorine for the epidemic sites of new coronavirus for 30 minutes could completely destroy the nucleic acid of new coronavirus. The Real-time quantitative PCR can be used to evaluate the disinfection effect of new coronavirus.