Chinese Journal of Experimental Traditional Medical Formulae 2019;25(1):16-21

doi:10.13422/j.cnki.syfjx.20190161

Establishment of DNA Fingerprints and Genetic Similarities Analysis of Dendrobium officinale

Wen-xia YU 1 ; Zhou-xi LEI 2 ; Yuan YUAN 3 ; Yu-lin WU 1 ; Qing-sheng SHUN 4 ; Gang WEI 1 ; Liu-ping HU 1 ; Ju-ting HU 1 ; Lu-qi HUANG 3

Affiliations

+expand

Keywords

Dendrobium officinale; DNA molecular identities; simple sequence repeats (SSR); genetic similarities

Country

China

Language

Chinese

Abstract

This study adopted the 4 pairs of simple sequence repeats(SSR) primers selected by National Resource Center for Chinese Meteria Medica (CACMS) to detect 29 populations of Dendrobium officinale samples from 9 main places of production; 5,7,4 and 3 polymorphism bands were amplified from these 4 pairs of SSR primers. The DNA identities of different populations were constructed by SSR. The 29 D. officinale populations could be divided into 4 classes. The clustering result was related to the places of production. Samples from Yunnan,Guizhou,Sichuan provinces were classified into one category,while samples from Anhui and Guangxi provinces were classified into another category. Samples from Guangdong Danxia,Zhejiang Yongkang,Zhejiang Leqing and Taining belonged to a category. PopGene (version 1.32) software was applied to calculate the genetic similarity of the 29 D. officinale populations. The similarities was between 0.403 4 and 1.0.Based on the genetic similarity,the genetic consistency included three classes,A,B and C. Samples with a similar geographical location and landform environment have higher genetic similarities,which indicate the same genetic background. This paper provides reference information to study the identification, selection and breeding of good varieties.