Chinese Journal of Cancer Biotherapy 2018;25(12):1264-1269

doi:10.3872/j.issn.1007-385x.2018.12.009

Inhibitory effect of recombinant oncolytic adenovirus on luciferase-labeled and non-labeled human lung cancerA549 cells

MA Yizhen 1 2 3 ; FAN Yuanyuan 1 2 3 ; NIE Xi 4 ; SUN Lili 5 ; ZHU Yilong 1 2 3 ; LI Yiquan 3 ; LI Wenjie 3 ; YIN Xunzhe 1 2 3 ; LI Shanzhi 3 ; ZHAO Jin 3 ; LI Xiao 3 ; GUO Yan 6 ; JIN Ningyi 3

Affiliations

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Keywords

oncolytic adenovirus; luciferase; A549-luc cell; inhibitory effect

Country

China

Language

Chinese

Abstract

Objective: To investigate the inhibitory effect of recombinant oncolytic adenovirusAd-Apoptin-hTERTp-E1A(ATV) on luciferase-labeled human lung cancer cells (A549-luc) and human lung cancerA549 cells, and to compare the differences in the inhibitory effect on two cell lines. Methods:ATV was used to infectA549-luc cells andA549 cells respectively. WST-1 and crystal violet staining were used to determine the difference in the inhibitory effect of ATV. Hoechst and Annexin V-FITC/PI staining were used to verify the inhibition mode ofATV. Results: WST-1 and crystal violet staining showed thatATV had significant inhibitory effect on bothA549-luc and A549 cells ( P <0.05). ATV showed significant inhibitory effect on both cells at 24, 48 and 72 h ( P <0.05 or P <0.01), and reached the peak at 72 h; ATV at concentrations of 1, 10 and 100 MOI all showed inhibitory effect on both cells, and reached the peak at 100 MOI. Hoechst staining showed that A549-luc cells and A549 cells infected with ATV showed typical nuclear fragmentation and marginal set. The results of Annexin V-FITC/PI Flow cytometry showed that ATV infection resulted in apoptosis of A549-luc and A549 cells, which was in a time-dependent manner and reached the peak at 72 h( P <0.05 or P <0.01). Conclusion: Insertion of luciferase didn’t significantly change the inhibitory effect and inhibitory mode ofATV onA549-luc cells.ATV exerted its in vitro inhibitory effect onA549-luc and A549 cells by inducing cell apoptosis.