PURPOSE: The aim of this study was to assess the physical properties of a novel resin-based endodontic sealer, Any-Seal, in comparison with AH Plus Jet. MATERIALS AND METHODS: Flow, radiopacity and compressive strength were examined according to ISO 6876/2001. For flow test, 0.05 mL of sealer was placed between glass plate and 100 g weight were applied. Ten minutes after mixing the sealers, the load was removed and the diameters of the compressed sealer discs were measured. For radiopacity, 10 mm diameter and 1 mm thickness sample were fabricated and took radiograph with an aluminum step-wedge and analyzed using imaging program. For compressive strength test, 4 mm × 6 mm cylindrical specimen was fabricated and tested after 24 hours and 1 week using Universal testing machine. RESULTS: Both tested sealers were consistent with ISO 6876/2001 in the flow and radiopacity test. The flow values of both sealers were not significantly different (P > 0.05). AH Plus Jet had significantly higher radiopacity (P < 0.05). AH Plus Jet showed higher compressive strength at both time intervals (P < 0.05). CONCLUSION: Any-Seal showed low compressive strength until after 1 week, so its physical and biological aspect should be evaluated more before clinical use.