Chinese Journal of Natural Medicines (English Ed.) 2019;17(10):778-784

doi:10.1016/S1875-5364(19)30094-9

Steroidal constituents from Helleborus thibetanus and their cytotoxicities.

Yu-Ze LI 1 ; Hua-Wei ZHANG 2 ; Hao FAN 2 ; Xiao-Fei LIANG 2 ; Bei SONG 1 ; Huan CHEN 1 ; Wen-Li HUANG 2 ; Zheng-Gang YUE 2 ; Xiao-Mei SONG 3 4 ; Jian-Li LIU 5

Affiliations

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Keywords

Cytotoxic activity; Helleborus thibetanus; Steroidal constituents; Structure identification

Country

China

Language

English

Abstract

Thibetanosides E-H (1-4), four new steroidal constituents including three rare sulfonates (2-4), were isolated from the roots and rhizomes of Helleborus thibetanus, together with nine known steroidal compounds (5-13). Their structures were elucidated by detailed spectroscopic analysis, including 1D and 2D NMR techniques and chemical evidence. In this study, compounds 2-13 were evaluated for their cytotoxic activities against HCT116, A549 and HepG2 tumor cell lines in vitro. Among them, compound 8 (thibetanoside C) showed cytotoxicities against A549 cells(IC 39.6 ± 1.9 μmol·L) and HepG2 cells(IC 41.5 ± 1.1 μmol·L), respectively. Compound 9 (23S, 24S)-24-[(O-β-D-fucopyranosyl)oxy]-3β, 23-dihydroxy-spirosta-5, 25(27)-diene-1β-ylO-(4-O-acetyl- α-L-rhamnopyranosyl)-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-α-L-arabinopyranoside) showed cytotoxicity against HCT116 cells(IC 33.6 ± 2.1 μmol·L).