Modern Hospital 2017;17(11):1665-1669

doi:10.3969/j.issn.1671-332X.2017.11.030

Structures and Antigenic Epitopes of Dust Mite Allergens: Der p 2 and Der f 2: a Comparative Study

Min LIANG 1 ; Xiao FENG ; La HU ; Jitao CHEN ; Ming LI ; Yuxiong LAI ; Zhaoyu LIU

Affiliations

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Keywords

Allergen Protein; Der p 2; Der f 2; B Cell Epitope; HLA High Affinity Site

Country

China

Language

Chinese

Abstract

Objective To compare and analyze the primary and secondary structures and antigenic epitopes of the two allergens: Der p 2 and Der f 2. Methods The protein sequences of Der p 2 and Der f 2 were downloaded online. The primary and secondary structures of the dust mite allergens were compared and analyzed bioinformatically to determine the potential epitope and signal peptide sites. Results Both Der p 2 and Der f 2 contained 146 amino acids and 9 potential protein binding sites with a secondary structure that mainly contains [3 - sheets, and there might be signal peptides site at the 1st 17th segment of the N - terminus. B cell epitopes analysis revealed that both Der p 2 and Der f 2 have 9 potential linear B epitopes and 2 conformational B epitopes. NetMHCⅡserver prediction showed Der p 2 contains 6 high affinity sites, whereas Der f 2 0nly contains 5. Conclusion This study may lay the foundation for further research of the biochemical function of the 2 allergens and contribute to vaccine development for allergen - specific immunotherapy.