Chinese Journal of Food Hygiene 2001;13(3):3-7

doi:10.3969/j.issn.1004-8456.2001.03.001

Tentative study on species-specific oligonucleotide probes for identification of five Bifidobacteria from human being

Yifan ZHANG 1 ; Lu RAN ; Xiuzhu DONG

Affiliations

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Country

China

Language

Chinese

Abstract

For rapidly identifying 5 bifidobacterium species (B.longum,B.breve,B.adolescentis,B.infantis and B.bifidum) often used in functional food,we designed five species-specific oligonucleotide sequences,which were based on the homology analysis of 16s rRNA genes.After being labeled with digoxigenin(DIG) at 3'-end,the sequences were used as probes hybridizing with DNA from these 5 bifidobacteria.These probes showed the desired species specificity,except that the probe for B.infantis had weak cross-reaction with B.breve.The result showed that these 16s rRNA gene probes were specific and effective tools for identifying and detecting Bifidobacteria in food.