Immune Network  2015;15(5):260-267

doi:10.4110/in.2015.15.5.260

Engagement of CD99 Reduces AP-1 Activity by Inducing BATF in the Human Multiple Myeloma Cell Line RPMI8226.

Minchan GIL 1 ; Hyo Kyung PAK ; Seo Jeong PARK ; A Neum LEE ; Young Soo PARK ; Hyangsin LEE ; Hyunji LEE ; Kyung Eun KIM ; Kyung Jin LEE ; Dok Hyun YOON ; Yoo Sam CHUNG ; Chan Sik PARK

Affiliations

+expand

Keywords

CD99; BATF; AP-1; Proliferation; MAP kinase

Country

Republic of Korea

Language

English

Abstract

CD99 signaling is crucial to a diverse range of biological functions including survival and proliferation. CD99 engagement is reported to augment activator protein-1 (AP-1) activity through mitogen-activated protein (MAP) kinase pathways in a T-lymphoblastic lymphoma cell line Jurkat and in breast cancer cell lines. In this study, we report that CD99 differentially regulated AP-1 activity in the human myeloma cell line RPMI8226. CD99 was highly expressed and the CD99 engagement led to activation of the MAP kinases, but suppressed AP-1 activity by inducing the expression of basic leucine zipper transcription factor, ATF-like (BATF), a negative regulator of AP-1 in RPMI8226 cells. By contrast, engagement of CD99 enhanced AP-1 activity and did not change the BATF expression in Jurkat cells. CD99 engagement reduced the proliferation of RPMI8226 cells and expression of cyclin 1 and 3. Overall, these results suggest novel CD99 functions in RPMI8226 cells.