International Journal of Biomedical Engineering 2018;41(6):465-469

doi:10.3760/cma.j.issn.1673-4181.2018.06.001

Effect of Olaparib on proliferation activity and radiosensitization of human non-small cell lung cancer cells

Yangyang KONG 1 ; Chang XU ; Xiaohui SUN ; Yan WANG ; Liqing DU ; Kaihua JI ; Ningning HE ; Yang LIU ; Jinhan WANG ; Qiang LIU

Affiliations

+expand

Keywords

Olaparib; Non-small cell lung cancer cells; Radiosensitization; Proliferation; DNA damage

Country

China

Language

Chinese

Abstract

Objective To investigate the effects of Olaparib on cell proliferation and radiosensitization of human non-small cell lung cancer cells.Methods Non-small cell lung H460 and H1299 cell lines were cultured in vitro and the cells in logarithmic growth phase were selected for experiments.MTT and colony formation assays were used to determine cell proliferation and radiosensitization,respectively.Single cell gel electrophoresis assay (comet assay) was used to detect irradiation-induced DNA damage.Results The results of MTT assay showed that Olaparib inhibited the proliferation of H460 and H1299 cells in a dose-dependent pattern (all P＜0.05).H1299 cell line was more sensitive to Olaparib than H460 cells.The results of colony formation assay showed that Olaparib enhanced the radiosensitizition of H460 and H1299 cells (all P＜0.05).The results of comet assay showed that Olaparib increased γ ray-induced DNA damage.Conclusions Olapani can enhance the radiosensitization of human non-small cell lung cancer cells,and the radiosensitization effect of Olaparib may be associated with the inhibition of cell proliferation and induction of irradiation-induced DNA damage.