Journal of Shenyang Medical College 2016;18(3):142-145

doi:10.16753/j.cnki.1008-2344.2016.03.005

Detecting the Expression of ST3GalⅤ in Human Hepatic Carcinoma Cell Lines by Sialyltransferase DNA Microarray

Yan ZHANG 1 ; Hao FU ; Yan GANG ; Yujie ZHAO ; Qun HE

Affiliations

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Keywords

DNA microarray; sialyltransferase; ST3GalⅤ; hepatic carcinoma

Country

China

Language

Chinese

Abstract

Objective: To investigate the differential expression of ST3GalⅤ in human hepatic carcinoma cell lines, HepG?2 and SMMC?7721 and normal hepatic cell line, L?02 by sialyltransferase DNA microarray. Methods: Expression of ST3Gal family was measured with DNA microarray. Western blot was used to verify DNA microarrays data. Result:ST3GalⅤ downregulated in HepG?2 and SMMC?7721 cell lines, compared with control cell line. Conclusion: The downregulation of ST3GalⅤ in HepG?2 and SMMC?7721 cell lines may result in the reduction of GD1a and influence the hepatocyte proliferations.