Mycobiology  2009;37(2):94-102

Culture Conditions for the Mycelial Growth of Ganoderma applanatum.

Woo Sik JO 1 ; Yun Ju CHO ; Doo Hyun CHO ; So Deuk PARK ; Young Bok YOO ; Soon Ja SEOK

Affiliations

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Keywords

Culture condition; Ganoderma applanatum; Medicinal mushroom

Country

Republic of Korea

Language

English

Abstract

Ganoderma applanatum is one of the most popular medicinal mushrooms due to the various biologically active components it produces. This study was conducted to obtain basic information regarding the mycelial culture conditions of Ganoderma applanatum. Based on the colony diameter and mycelial density, PDA, YMA and MCM media were suitable for the mycelial growth of the mushroom. The optimum temperature for mycelial growth was found to be 25~30degrees C. The optimum carbon and nitrogen sources were mannose and dextrin, respectively, and the optimum C/N ratio was 2 to 10 when 2% glucose was used. Other minor components required for the optimal growth included thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and MgSO4.7H2O, KH2PO4 and NaCl as mineral salts.