The Korean Journal of Physiology and Pharmacology  2010;14(2):71-75

doi:10.4196/kjpp.2010.14.2.71

Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei.

Xiang Mei YAN 1 ; Chung Hyo KIM ; Chul Kyu LEE ; Jang Sik SHIN ; Il Hwan CHO ; Uy Dong SOHN

Affiliations

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Keywords

Eisenia andrei; Fibrinolytic enzymes; Intestinal proteinase absorption; Gut sac; Recirculation intestinal perfusion

Country

Republic of Korea

Language

English

Abstract

To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI-TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.