BACKGROUND Cigarette smoking is known to reduce appetite and body weight. Even though number of studies explored different levels of effects of smoking, there are few studies which address short-term effect of smoking on metabolism. METHODS Inbred strain, Balb/cmice (n=20) were used. Mice were divided into two groups control (n=7) and treatment (n=9) group. Smoking treatment conducted in 5 days consequently in treatment group, three times a day with cigarette smoke. During experiment both control and treatment groups were monitored for food intake, water intake, body weight. RESULTS In the end of the experiment blood glucose and anxiety levels were measured. In addition, liver, white adipose tissue and brown adipose issue were sampled comparison. Short-term (5 days) treatment of smoking of treatment group result in significant difference in food and water intake (p<0.05) as well as tendency to lowering blood glucose and reduction of mesenterial, perirenal, epidydimal, white adipose tissues and brown adipose revealed tendency to reduction of mesenterial white adipose with control group. (Mes. White adipose tissue weights 0.44g in control group, 0.23g in treatment group). CONCLUSION There was not significant difference in blood glucose test and anxiety test evaluated by time spent on alleys and transition between alleys in two groups.