Fudan University Journal of Medical Sciences 2017;44(6):799-805

doi:10.3969/j.issn.1672-8467.2017.06.017

Resolution revolution:advances and prospects of cryo-EM in structural biology

guo Zhen CHEN 1 ; 复旦大学附属上海市第五人民医院 上海 200240 ; hui Yan XU

Affiliations

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Keywords

cryo electron microscopy; structural biology; single particle reconstruction

Country

China

Language

Chinese

Abstract

Cryo electron microscopy (cryo-EM) is one of the most important methods in structural biology.In the past five years,cryo-EM has milestone breakthrough due to revolutionary advances in hardware and methodology.The resolution has been pushed to as high as 1.8 （A),which significantly extended the research scope of this method.Single particle reconstruction method has become one of the most exciting fields of structural biology and related subjects.Here we will briefly introduce the history of cryo-EM,recent revolutionary breakthrough,and the facility at Fudan University.