Journal of Audiology and Speech Pathology 2017;25(6):630-633

doi:10.3969/j.issn.1006-7299.2017.06.019

The Expression of Cav1.3 Calcium Channels in Adult Rat Cochlea

Jin CHEN 1 ; Liangqiang ZHOU ; Hanqi CHU ; Qingguo CHEN ; Zhihui DU ; Fan QI ; Yun LIU ; Yanbo SUN ; Pengjun LI ; Dan BING

Affiliations

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Keywords

Cochlea; Cav 1.3 calcium channel; Rat

Country

China

Language

Chinese

Abstract

Objective To investigate the expression of Cav 1.3 calcium channel in adult rat cochlea and study its role in auditory physiology and pathology.Methods The sprague-dawley rats were used as experimental subjects.The distribution of Cav1.3 calcium channel in the cochlea was detected by immunofluorescence technique.The expression of Cav1.3 was measured with Western blot (WB) and RT-PCR.Results Immunofluorescence photographs revealed that Cav 1.3 calcium channel localized in the lateral wall membrane,hair cells,stria vascularis,spiral ganglion cell,spiral ligment,spiral prominence,and limbus laminae spiralis.The results of WB and RT-PCR inform Cav1.3 calcium channel gene (CACNA1D) were measured in the cochlea and kidney.The expression of Cav1.3was mainly in the basilar membrane.Moderate expression was observed in the spiral ganglion and stria vascularis.Conclusion The preliminary study revealed the distribution of Cav 1.3 calcium channel gene(CACNA1D)in adult rat cochle possesses tissue specificity,providing a theoretical basis for further research in auditory physiology and pathology.