Journal of Forensic Medicine 2017;33(4):339-343

doi:10.3969/j.issn.1004-5619.2017.04.001

Gene Analysis for the Sudden Death of Hypertrophic Cardiomyopathy by Whole Exome Sequencing

chao Chuan XU 1 ; zhi Yun BAI ; shu Xin XU ; li Guo L(U) ; ping Xiao LAI ; Rui CHEN ; guang Han LIN ; jian Wen KUANG

Affiliations

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Keywords

forensic pathology; cardiomyopathy,hypertrophic; death,sudden,cardiac; exome; mutation

Country

China

Language

Chinese

Abstract

Objective To analyze the related pathogenicity gene mutations in a sudden death of hypertrophic cardiomyopathy (HCM) on whole exome level.Methods Whole exome sequencing (WES) was been performed on a sudden death case sample with pathological features of HCM by Illumina(R) Hiseq 2500 platform.Using hgl9 as the reference sequences,the sequencing data were analyzed.Suspicious single nucleotide variants (SNV) were screened,and the conservatism and function were analyzed by the software such as PhyloP,PolyPhen-2,SIFT,etc.Results After screening,a heterozygous mutation C719R was finally identified in the gene MYBPC3 of this case.Conclusion The molecular anatomy on whole exome level by second generation sequencing technology can help to define the molecular mechanism of HCM and provide a new mothed and thought for analysis of death cause.