Objective To evaluate the efficacy of bladder perfusion with sodium hyaluronate combined with ultrashort wave irradiation in bladder region in treatment of glandular cystitis.Methods 22 patients diagnosed as typical glandular cystitis were selected from our hospital between March 2013 and March 2013. Those patients were given bladder perfusion with sodium hyaluronate combined with ultrashort wave irradiation in bladder region according to the designed course. The lesion areas were taked photos and bited samples for microscopic observation and HE staining.The evaluation indicators include pelvic pain and urgency/frequency symptom scores,cystitis histologic scores ,infiltrating lymphocyte counts, Brunn nest or cystica counts and residual urine volume were measured in the self-control expertiment before treatment and after. Results Over the course of treatment, above mentioned indicators had significant improvement compared with original data among those patients (P<0.01).Only 1 patient appeared itchy skin two times after bladder perfusion with sodium hyaluronate, others without serious complications. Conclusions The method that bladder perfusion with sodium hyaluronate combined with ultrashort wave irradiation has clear effect without serious complications.This method provides a new train of thought for glandular cystitis and opens up a new space.