Objective To establish HPLC characteristic chromatograms method for quality control of Saponins in Panacis Majoris Rhizoma.Methods The HPLC method was conducted based on a Agilent Poroshell 120 C18 (4.6 mm × 100 mm, 2.7μm) column, with acetonitrile-0.1％ orthophosphoric acid as mobile phase by gradient elution. The flow rate was 1.0 ml/min. The detection wavelength was at 203 nm and the column temperature was 35℃. Results Eight samples from different sources were analyzed by HPLC. Fourteen main marker peaks were selected in the Characteristic spectrum and four peaks of saponins were identified. The similarity of seven samples was over 0.9.Conclusions The precision, repeatability and stability method was standardized, and this method can be used for quality control of Panacis Majoris Rhizoma.