Objective To analyze the method and effect of ultrasonic diagnosis in percutaneous hepatocholangiostomy, and to explore its clinical application value. Methods Twenty-one cases of patients who used intraoperative ultrasound in percutaneous hepatocholangiostomy from December 2014 to October 2016 were selected as experimental group. Twenty-three cases of patients who didn′t used intraoperative ultrasound in percutaneous hepatocholangiostomy within the same period were selected as control group. At the end, the treatment effect of the two groups were compared. Results In experimental group, the success rate of fistula was 100.0%(21/21), the mean operation time was (95.6 ± 20.7)min, the average bleeding was(7.4 ± 5.9)ml, the rate of stone clearance was 100.0%, and the rate of complication was 0. In control group, fistula success rate was 73.9%(17/23), average operation time was (117.8 ± 32.1) min, average bleeding during operation was (16.1 ± 13.3) ml, stone clearance rate was 78.3%, and complication rate was 8.7%.There were significant differences between two groups (P < 0.05). Conclusions Ultrasonic diagnosis used in percutaneous hepatocholangiostomy can significantly enhance the rate of calculus, and improve the success rate of fistula.This operation is simple and convenient, and clinical effect is better.