Journal of Forensic Medicine 2017;33(5):514-515,521

doi:10.3969/j.issn.1004-5619.2017.05.014

PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone

cheng Shao DING 1 ; cai Huai ZHANG ; lin Lin GAO

Affiliations

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Keywords

forensic genetics; bone; DNA; PrepFiler Express BTATM; silica-extraction method

Country

China

Language

Chinese

Abstract

Objective To establish a convenient and rapid method for extracting DNA from bone.Methods Fifteen long bone samples were washed and sterilized.The skeletal fragments were obtained by electric drill,and lysed by PrepFiler Express BTATM lysis buffer.DNA was then manually extracted by silicon microbeads for further analysis.Results STR genotyping was successfully obtained in 14 out of the 15 samples,and the detection rate was 93.33％.Conclusion The method for DNA extraction from bone established in present study is convenient,quick,effective,and with a strong applicability,which is worth spreading and applying.