Objective To establish a method for the analysis of methcathinone in urine by gas chromatography-mass spectrometry (GC-MS).Methods Proadifen hydrochloride (internal standard) and buffer solution (pH=9) were added into the urine samples,and methcathinone was extracted by ethyl acetate.The extract was volatilized in 50 ℃ nitrogen gas flow and the remnant was dissolved by methanol and analysed by GC-MS.Results The methcathinone in urine showed a good linear relationship in the mass concentration range of 0.02-2.00 μg/mL.The linear equation was y=0.301 9x+0.0189 (r=0.9992),and the detection limit was 0.01 μg/mL.The recoveries of methcathinone in urine was 96.4％-99.2％,with the intraday precision of 5.8％-7.6％ and the inter-day precision of 6.0％-8.1％.Conclusion The method is convenient and sensitive,which can be applied to the forensic identification of methcathinone in urine.