Objective To investigate the effect of a stabilization device for maintaining the balance of a cardiopulmonary resuscitation (CPR) performer during ambulance transportation on quality of CPR in out-of-hospital cardiac arrest (OHCA).Methods A prospective randomized controlled trial was performed. 167 OHCA patients with cardiac arrest (CA) time < 10 minutes admitted to Cangzhou Central Hospital from October 2014 to January 2017 were enrolled, and divided into armed stabilization device group (n = 86) and unarmed stabilization device group (n = 81) by random number table. Restoration of spontaneous circulation (ROSC) rate, 24-hour survival rate and survival rate of discharge were evaluated.Results Compared with unarmed stabilization device group, ROSC rate (29.1％ vs. 9.9％,χ2 = 9.691,P = 0.002), 24-hour survival rate (20.9％ vs. 6.2％,χ2 = 7.649,P = 0.006) and survival rate of discharge (12.8％ vs. 3.7％,χ2 = 4.485,P = 0.035) were significant increased in armed stabilization device group. Conclusion CPR with stabilization device during ambulance transport could effectively ensure quality of CPR and improve prognosis in OHCA.Clinical Trial Registration Chinese Clinical Trial Registry, ChiCTR-IPR-14005337.