Korean Journal of Anatomy  2003;36(4):257-264

Quantitative Analysis of Agmatine by HPLC in Ischemic Brain.

Jong Soon HONG 1 ; Hyug CHUN ; Hyo Seok JEONG ; Jae Hwan KIM ; Won Taek LEE ; Kyung Ah PARK ; Jong Eun LEE

Affiliations

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Keywords

Agmatine; Cerebral ischemia; Reperfusion; Neuroprotective effect; MCAO

Country

Republic of Korea

Language

Korean

Abstract

Agmatine, a widely distributed molecule in mammalian tissues, shows neuroprotective effects in brain ischemia. We describe the neuroprotective effects of agmatine in the mouse MCAO model and the quantitative change of agmatine in ischemic injury. Brain ischemic injured mice were injected with agmatine (100 mg/kg of mouse, IP). Agmatine significantly reduced the infarct area after MCAO. Despite the similar patterns of agmatine change observed in control or agmatine injected animals, the agmatine levels of the penumbra were significantly higher than those of the striatum and the cerebral cortex during the early period (<1 hour after 2 hours of MCA occlusion). This suggests that the early period, during which agmatine levels increase in the brain, is the crucial period in terms of neuroprotective effect during ischemia.