Korean Journal of Anatomy  2002;35(2):173-177

Immunocytochemical Localization of GABA Transporter-3 in the Guinea Pig Retina.

Eun Jin LEE 1 ; Su Ja OH ; Jin Woong CHUNG ; Myung Hoon CHUN

Affiliations

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Keywords

GABA transporter-3; GAD-65; CD15; Amacrine cells; Immunocytochemistry; Retina; Guinea pig

Country

Republic of Korea

Language

English

Abstract

The cellular localization of the GABA transporter-3 (GAT-3) was examined in the guinea pig retina by immunocytochemistry, using antisera against GAT-3. GAT-3 immunoreactivity was localized to cell bodies in the inner nuclear layer, and labeled processes were densely distributed in the inner plexiform layer (IPL) close to the ganglion cell layer. All GAT-3 labeled cells exhibited GAD65 immunoreactivity. In addition, 67% of GAT-3 labeled amacrine cells showed carbohydrate epitope CD15 immunoreactivity. These results indicate that GAT-3 is involved in modulating the rod pathway in the IPL of the guinea pig retina via presumptive A17 amacrine cells.